Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture
ABSTRACT Objective This study aimed to evaluate the effects of different concentrations of vegetable peptones (pea, wheat, and soy) as substitutes for fetal bovine serum in stem cell cultures derived from human exfoliated deciduous teeth. Methods Stem cell cultures derived from human exfoliated...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Israelita de Ensino e Pesquisa Albert Einstein
2025-08-01
|
| Series: | Einstein (São Paulo) |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082025000100239&lng=en&tlng=en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849771456400982016 |
|---|---|
| author | Marizia Trevizani Laís Lopardi Leal Silvioney Augusto da Silva Claudio Gallupo Diniz Fabiano Freire Costa Jair Adriano Kopke de Aguiar Carlos Magno da Costa Maranduba |
| author_facet | Marizia Trevizani Laís Lopardi Leal Silvioney Augusto da Silva Claudio Gallupo Diniz Fabiano Freire Costa Jair Adriano Kopke de Aguiar Carlos Magno da Costa Maranduba |
| author_sort | Marizia Trevizani |
| collection | DOAJ |
| description | ABSTRACT Objective This study aimed to evaluate the effects of different concentrations of vegetable peptones (pea, wheat, and soy) as substitutes for fetal bovine serum in stem cell cultures derived from human exfoliated deciduous teeth. Methods Stem cell cultures derived from human exfoliated deciduous teeth were cultured with peptones in different concentrations [0.5%, 1%, and 5% (w/v)] and 10% fetal bovine serum (v/v) as control, and their proliferation was evaluated through the 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide assay. Osteogenic differentiation was assessed using Alizarin Red to quantify calcium deposition. Results Wheat, soy, and pea concentrations greater than 1% were cytotoxic to stem cell cultures derived from human exfoliated deciduous teeth. In addition, a long-term study showed that pea peptones were cytotoxic. Studies with soy and wheat peptones were continued at concentrations of 0.5% (w/v), and proliferation on day 3 was greater than 50% compared with the control. Wheat peptone presented more mineralized areas than fetal bovine serum. The aminograms of the three peptones showed that the greater efficiency of wheat peptone may be related to its higher proline and glutamic acid proportions. Conclusion We suggest that vegetable peptones at concentrations ≤1%, particularly 1% wheat, can be used as fetal bovine serum substitutes for stem cell cultures derived from human exfoliated deciduous teeth cultivation. |
| format | Article |
| id | doaj-art-b34837bef35c45f49a6aad1dfd17c1f0 |
| institution | DOAJ |
| issn | 2317-6385 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj-art-b34837bef35c45f49a6aad1dfd17c1f02025-08-20T03:02:37ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852025-08-012310.31744/einstein_journal/2025ao1364Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell cultureMarizia Trevizanihttps://orcid.org/0000-0003-1227-9507Laís Lopardi Lealhttps://orcid.org/0000-0001-8144-302XSilvioney Augusto da Silvahttps://orcid.org/0009-0003-3511-2045Claudio Gallupo Dinizhttps://orcid.org/0000-0001-6882-8312Fabiano Freire Costahttps://orcid.org/0000-0002-7206-6792Jair Adriano Kopke de Aguiarhttps://orcid.org/0000-0003-2088-8871Carlos Magno da Costa Marandubahttps://orcid.org/0000-0001-7327-1934ABSTRACT Objective This study aimed to evaluate the effects of different concentrations of vegetable peptones (pea, wheat, and soy) as substitutes for fetal bovine serum in stem cell cultures derived from human exfoliated deciduous teeth. Methods Stem cell cultures derived from human exfoliated deciduous teeth were cultured with peptones in different concentrations [0.5%, 1%, and 5% (w/v)] and 10% fetal bovine serum (v/v) as control, and their proliferation was evaluated through the 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide assay. Osteogenic differentiation was assessed using Alizarin Red to quantify calcium deposition. Results Wheat, soy, and pea concentrations greater than 1% were cytotoxic to stem cell cultures derived from human exfoliated deciduous teeth. In addition, a long-term study showed that pea peptones were cytotoxic. Studies with soy and wheat peptones were continued at concentrations of 0.5% (w/v), and proliferation on day 3 was greater than 50% compared with the control. Wheat peptone presented more mineralized areas than fetal bovine serum. The aminograms of the three peptones showed that the greater efficiency of wheat peptone may be related to its higher proline and glutamic acid proportions. Conclusion We suggest that vegetable peptones at concentrations ≤1%, particularly 1% wheat, can be used as fetal bovine serum substitutes for stem cell cultures derived from human exfoliated deciduous teeth cultivation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082025000100239&lng=en&tlng=enPeptonesVegetablesToothdeciduousDental pulp diseasesSerum fetal , bovineCell- and tissue-based therapySteam cellsCulture |
| spellingShingle | Marizia Trevizani Laís Lopardi Leal Silvioney Augusto da Silva Claudio Gallupo Diniz Fabiano Freire Costa Jair Adriano Kopke de Aguiar Carlos Magno da Costa Maranduba Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture Einstein (São Paulo) Peptones Vegetables Tooth deciduous Dental pulp diseases Serum fetal , bovine Cell- and tissue-based therapy Steam cells Culture |
| title | Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture |
| title_full | Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture |
| title_fullStr | Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture |
| title_full_unstemmed | Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture |
| title_short | Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture |
| title_sort | vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture |
| topic | Peptones Vegetables Tooth deciduous Dental pulp diseases Serum fetal , bovine Cell- and tissue-based therapy Steam cells Culture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082025000100239&lng=en&tlng=en |
| work_keys_str_mv | AT mariziatrevizani vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture AT laislopardileal vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture AT silvioneyaugustodasilva vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture AT claudiogallupodiniz vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture AT fabianofreirecosta vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture AT jairadrianokopkedeaguiar vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture AT carlosmagnodacostamaranduba vegetablepeptonesasafetalbovineserumsubstituteinhumandeciduoustoothpulpstemcellculture |