CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation
Introduction: Chlamydia trachomatis is the leading cause of sexually transmitted bacterial disease, which may cause significant threats, such as pelvic inflammatory disease and tubal factor infertility, to women if untreated. The pathological mechanisms of chlamydia-induced disease remain largely u...
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The Journal of Infection in Developing Countries
2017-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9356 |
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| author | Yuyang Zhang Guangming Zhong Huihua Cai Siping Chen Donghua Sun Dongmei Zhang Yuanli He |
| author_facet | Yuyang Zhang Guangming Zhong Huihua Cai Siping Chen Donghua Sun Dongmei Zhang Yuanli He |
| author_sort | Yuyang Zhang |
| collection | DOAJ |
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Introduction: Chlamydia trachomatis is the leading cause of sexually transmitted bacterial disease, which may cause significant threats, such as pelvic inflammatory disease and tubal factor infertility, to women if untreated. The pathological mechanisms of chlamydia-induced disease remain largely unknown, but it has been proposed that CPAF, a chlamydia-secreted serine protease, may play major roles in aiding chlamydial infection and contribute to chlamydia pathogenesis during in vivo infection. According to previous results, CPAF targets host immunity by degrading antimicrobial peptides and neutralizing complement activity; however, whether CPAF is involved in chlamydial antigen presentation has never been reported.
Methodology: Antigen presentation assay was used to monitor the effects of CPAF on OT1-, OT2-, and chlamydia T cell antigen-mediated antigen presentation. In vitro cell-free degradation assay was used to detect CPAF processing of chlamydia T cell antigens.
Results: We found that CPAF preferably inhibits OT2- but not OT1-mediated antigen presentation. CPAF inhibits OT2 antigen presentation by direct proteolytic cleavage in the wild type CPAF, but not enzymatic mutants. Importantly, several previously identified chlamydial T cell antigens were selectively degraded by CPAF when co-incubated in vitro. In addition, specific inhibition T cell antigen presentation by CPAF was correlated with T cell antigen cleavage by CPAF in vitro assay.
Conclusions: Our experiments demonstrated that CPAF selectively and specifically degrades chlamydial T cell antigens, which chlamydia may utilize as a novel mechanism for evading host immune responses to promote chlamydia survival.
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| format | Article |
| id | doaj-art-b347258392fa449885cdeb76d8daf336 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b347258392fa449885cdeb76d8daf3362025-08-20T02:16:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-12-01111110.3855/jidc.9356CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentationYuyang Zhang0Guangming Zhong1Huihua Cai2Siping Chen3Donghua Sun4Dongmei Zhang5Yuanli He6Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaUniversity of Texas Health Science Center at San Antonio, San Antonio, United StatesZhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaZhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaZhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaZhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaZhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China Introduction: Chlamydia trachomatis is the leading cause of sexually transmitted bacterial disease, which may cause significant threats, such as pelvic inflammatory disease and tubal factor infertility, to women if untreated. The pathological mechanisms of chlamydia-induced disease remain largely unknown, but it has been proposed that CPAF, a chlamydia-secreted serine protease, may play major roles in aiding chlamydial infection and contribute to chlamydia pathogenesis during in vivo infection. According to previous results, CPAF targets host immunity by degrading antimicrobial peptides and neutralizing complement activity; however, whether CPAF is involved in chlamydial antigen presentation has never been reported. Methodology: Antigen presentation assay was used to monitor the effects of CPAF on OT1-, OT2-, and chlamydia T cell antigen-mediated antigen presentation. In vitro cell-free degradation assay was used to detect CPAF processing of chlamydia T cell antigens. Results: We found that CPAF preferably inhibits OT2- but not OT1-mediated antigen presentation. CPAF inhibits OT2 antigen presentation by direct proteolytic cleavage in the wild type CPAF, but not enzymatic mutants. Importantly, several previously identified chlamydial T cell antigens were selectively degraded by CPAF when co-incubated in vitro. In addition, specific inhibition T cell antigen presentation by CPAF was correlated with T cell antigen cleavage by CPAF in vitro assay. Conclusions: Our experiments demonstrated that CPAF selectively and specifically degrades chlamydial T cell antigens, which chlamydia may utilize as a novel mechanism for evading host immune responses to promote chlamydia survival. https://jidc.org/index.php/journal/article/view/9356ChlamydiaCPAFantigen presentationhost immunity |
| spellingShingle | Yuyang Zhang Guangming Zhong Huihua Cai Siping Chen Donghua Sun Dongmei Zhang Yuanli He CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation Journal of Infection in Developing Countries Chlamydia CPAF antigen presentation host immunity |
| title | CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation |
| title_full | CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation |
| title_fullStr | CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation |
| title_full_unstemmed | CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation |
| title_short | CPAF selectively degrades chlamydial T cell antigens for inhibiting antigen presentation |
| title_sort | cpaf selectively degrades chlamydial t cell antigens for inhibiting antigen presentation |
| topic | Chlamydia CPAF antigen presentation host immunity |
| url | https://jidc.org/index.php/journal/article/view/9356 |
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