Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season
Background: Numerous plant and animal species have β-defensins, antimicrobial peptides involved in immunity and reproduction. Beta-defensin 126 (DEFB126) belongs to the β-defensin family and is a vital component of sperm function. It regulates the capacitation and sperm-egg interaction. Aim: The...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2025-03-01
|
| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=229123 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849729480899166208 |
|---|---|
| author | Khalid M. Al Khodair Abdulkarem Al-Shabebi Thnaian A. Al-Thnaian Osamah I. M. Alturki Marwa-Babiker A.M. Saeed Y Al-Ramadan Abdelhay M. Ali Abdelrahman M.A. Elseory |
| author_facet | Khalid M. Al Khodair Abdulkarem Al-Shabebi Thnaian A. Al-Thnaian Osamah I. M. Alturki Marwa-Babiker A.M. Saeed Y Al-Ramadan Abdelhay M. Ali Abdelrahman M.A. Elseory |
| author_sort | Khalid M. Al Khodair |
| collection | DOAJ |
| description | Background:
Numerous plant and animal species have β-defensins, antimicrobial peptides involved in immunity and reproduction. Beta-defensin 126 (DEFB126) belongs to the β-defensin family and is a vital component of sperm function. It regulates the capacitation and sperm-egg interaction.
Aim:
There is no evidence of DEFB126 expression in the dromedary camel's sperm or reproductive tract.
Methods:
The current work used immunohistochemical (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR) methods to clarify the distribution and expression of DEFB126 in the sperm and male reproductive tract (MRT) of dromedary camels in the time of rutting. Samples of fresh and epididymal sperm, testicular, epididymis, ductus deferens, and male accessory gland tissues were obtained from twenty male camels.
Results:
The results of IHC showed that the fresh and epididymal sperms had a positive immunoreaction to DEFB126 antibodies. Also, all parts of the testicles, epididymis, vas deferens, prostate, and bulbourethral glands were positively stained to various degrees with a strong immunoreaction in the epididymal's tail. qRT-PCR results showed that expression levels of DEFB126 mRNA varied in the fresh and epididymal sperms and throughout all parts of the MRT; the tail of the epididymis had the most significant expression levels (P [Open Vet J 2025; 15(3.000): 1206-1216] |
| format | Article |
| id | doaj-art-b3364f9a9bf848368b89bb26a7d47c32 |
| institution | DOAJ |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-b3364f9a9bf848368b89bb26a7d47c322025-08-20T03:09:12ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502025-03-011531206121610.5455/OVJ.2025.v15.i3.12229123Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting seasonKhalid M. Al Khodair0Abdulkarem Al-Shabebi1Thnaian A. Al-Thnaian2Osamah I. M. Alturki3Marwa-Babiker A.M.4Saeed Y Al-Ramadan5Abdelhay M. Ali6Abdelrahman M.A. Elseory71- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 2- Department of Animal Resources, Ministry of Municipality, Doha, 2713. Qatar 3- Veterinary Department, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar 2153, Yemen. 1- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 1- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 1- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 2- Department of Anatomy, College of Veterinary Medicine, University of Bahri, P.O. Box 012, Khartoum North 12217, Sudan. 1- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 1- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 1- Department of Anatomy, College of Veterinary Medicine, king Faisal University, Al-Ahsa, Saudi Arabia 2- Department of Anatomy, Faculty of Veterinary Medicine, University of Khartoum. PO Box: 32, Postal code: 13314 Shambat, Khartoum North, Sudan.Background: Numerous plant and animal species have β-defensins, antimicrobial peptides involved in immunity and reproduction. Beta-defensin 126 (DEFB126) belongs to the β-defensin family and is a vital component of sperm function. It regulates the capacitation and sperm-egg interaction. Aim: There is no evidence of DEFB126 expression in the dromedary camel's sperm or reproductive tract. Methods: The current work used immunohistochemical (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR) methods to clarify the distribution and expression of DEFB126 in the sperm and male reproductive tract (MRT) of dromedary camels in the time of rutting. Samples of fresh and epididymal sperm, testicular, epididymis, ductus deferens, and male accessory gland tissues were obtained from twenty male camels. Results: The results of IHC showed that the fresh and epididymal sperms had a positive immunoreaction to DEFB126 antibodies. Also, all parts of the testicles, epididymis, vas deferens, prostate, and bulbourethral glands were positively stained to various degrees with a strong immunoreaction in the epididymal's tail. qRT-PCR results showed that expression levels of DEFB126 mRNA varied in the fresh and epididymal sperms and throughout all parts of the MRT; the tail of the epididymis had the most significant expression levels (P [Open Vet J 2025; 15(3.000): 1206-1216]http://www.ejmanager.com/fulltextpdf.php?mno=229123cameldefb126immunohistochemistrymale reproductive tractqrt-pcrsperm |
| spellingShingle | Khalid M. Al Khodair Abdulkarem Al-Shabebi Thnaian A. Al-Thnaian Osamah I. M. Alturki Marwa-Babiker A.M. Saeed Y Al-Ramadan Abdelhay M. Ali Abdelrahman M.A. Elseory Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season Open Veterinary Journal camel defb126 immunohistochemistry male reproductive tract qrt-pcr sperm |
| title | Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season |
| title_full | Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season |
| title_fullStr | Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season |
| title_full_unstemmed | Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season |
| title_short | Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Camelus dromedarius) during the rutting season |
| title_sort | beta defensin 126 defb126 localization and expression in the sperms and male reproductive tract of the dromedary camel camelus dromedarius during the rutting season |
| topic | camel defb126 immunohistochemistry male reproductive tract qrt-pcr sperm |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=229123 |
| work_keys_str_mv | AT khalidmalkhodair betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT abdulkaremalshabebi betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT thnaianaalthnaian betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT osamahimalturki betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT marwababikeram betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT saeedyalramadan betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT abdelhaymali betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason AT abdelrahmanmaelseory betadefensin126defb126localizationandexpressioninthespermsandmalereproductivetractofthedromedarycamelcamelusdromedariusduringtheruttingseason |