Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018

The recent emergence of plasmid-mediated tigecycline resistance genes, tet(X3) and tet(X4), in animals and humans in China would pose a foreseeable threat to public health. To illustrate this paradigm shift in tigecycline resistance, here, covering the period 2008-2018, we retrospectively analysed a...

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Main Authors: Chengtao Sun, Mingquan Cui, Shan Zhang, Hejia Wang, Li Song, Chunping Zhang, Qi Zhao, Dejun Liu, Yang Wang, Jianzhong Shen, Shixin Xu, Congming Wu
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Emerging Microbes and Infections
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Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2019.1678367
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author Chengtao Sun
Mingquan Cui
Shan Zhang
Hejia Wang
Li Song
Chunping Zhang
Qi Zhao
Dejun Liu
Yang Wang
Jianzhong Shen
Shixin Xu
Congming Wu
author_facet Chengtao Sun
Mingquan Cui
Shan Zhang
Hejia Wang
Li Song
Chunping Zhang
Qi Zhao
Dejun Liu
Yang Wang
Jianzhong Shen
Shixin Xu
Congming Wu
author_sort Chengtao Sun
collection DOAJ
description The recent emergence of plasmid-mediated tigecycline resistance genes, tet(X3) and tet(X4), in animals and humans in China would pose a foreseeable threat to public health. To illustrate this paradigm shift in tigecycline resistance, here, covering the period 2008-2018, we retrospectively analysed a national strain collection of Escherichia coli (n = 2254), obtained from chickens and pigs, in six representative provinces of China. The gene tet(X4) was identified in five pig isolates collected in 2016 and 2018 from the provinces of Sichuan (3/15, 2018), Henan (1/25, 2018) and Guangdong (1/28, 2016), but not in the isolates prior to 2016. None of the isolates was detected harbouring tet(X3). All tet(X4)-positive E. coli exhibited high levels of tigecycline resistance (MICs, 16-64 mg/L), and two were confirmed as colistin resistant, harbouring chromosome-borne mcr-1 gene. The gene tet(X4) was detected on a plasmid in all five isolates, whereas a co-location of tet(X4) on the chromosome of one isolate was observed. Diverse host strains and novel plasmids related to the tet(X4) gene were observed. Our timely findings of the recent emergence of tet(X4) gene in food animal support the rapid surveillance and eradication of this gene before it is established.
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spelling doaj-art-b32ea24344384573b06695c120163e132025-08-20T03:17:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811524152710.1080/22221751.2019.1678367Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018Chengtao Sun0Mingquan Cui1Shan Zhang2Hejia Wang3Li Song4Chunping Zhang5Qi Zhao6Dejun Liu7Yang Wang8Jianzhong Shen9Shixin Xu10Congming Wu11Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaChina Institute of Veterinary Drug Control, Beijing, People’s Republic of ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaChina Institute of Veterinary Drug Control, Beijing, People’s Republic of ChinaChina Institute of Veterinary Drug Control, Beijing, People’s Republic of ChinaChina Institute of Veterinary Drug Control, Beijing, People’s Republic of ChinaChina Institute of Veterinary Drug Control, Beijing, People’s Republic of ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaChina Institute of Veterinary Drug Control, Beijing, People’s Republic of ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaThe recent emergence of plasmid-mediated tigecycline resistance genes, tet(X3) and tet(X4), in animals and humans in China would pose a foreseeable threat to public health. To illustrate this paradigm shift in tigecycline resistance, here, covering the period 2008-2018, we retrospectively analysed a national strain collection of Escherichia coli (n = 2254), obtained from chickens and pigs, in six representative provinces of China. The gene tet(X4) was identified in five pig isolates collected in 2016 and 2018 from the provinces of Sichuan (3/15, 2018), Henan (1/25, 2018) and Guangdong (1/28, 2016), but not in the isolates prior to 2016. None of the isolates was detected harbouring tet(X3). All tet(X4)-positive E. coli exhibited high levels of tigecycline resistance (MICs, 16-64 mg/L), and two were confirmed as colistin resistant, harbouring chromosome-borne mcr-1 gene. The gene tet(X4) was detected on a plasmid in all five isolates, whereas a co-location of tet(X4) on the chromosome of one isolate was observed. Diverse host strains and novel plasmids related to the tet(X4) gene were observed. Our timely findings of the recent emergence of tet(X4) gene in food animal support the rapid surveillance and eradication of this gene before it is established.https://www.tandfonline.com/doi/10.1080/22221751.2019.1678367Mobile tigecycline resistanceEscherichia colifood-producing animalsretrospective analysisChina
spellingShingle Chengtao Sun
Mingquan Cui
Shan Zhang
Hejia Wang
Li Song
Chunping Zhang
Qi Zhao
Dejun Liu
Yang Wang
Jianzhong Shen
Shixin Xu
Congming Wu
Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018
Emerging Microbes and Infections
Mobile tigecycline resistance
Escherichia coli
food-producing animals
retrospective analysis
China
title Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018
title_full Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018
title_fullStr Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018
title_full_unstemmed Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018
title_short Plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from food-producing animals, China, 2008–2018
title_sort plasmid mediated tigecycline resistant gene tet x4 in escherichia coli from food producing animals china 2008 2018
topic Mobile tigecycline resistance
Escherichia coli
food-producing animals
retrospective analysis
China
url https://www.tandfonline.com/doi/10.1080/22221751.2019.1678367
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