Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields

Different fertilization measures affect the soil’s physical and chemical properties and bacterial community structure, which in turn affects the growth environment and yield of maize seed production. Therefore, rational fertilization measures are important in maintaining and improving soil fertility...

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Main Authors: Yirong Yang, Zhenhua Zhao, Bo Dong, Rui Zhang, Jing Jiang, Fengjie Ma, Yingying Zhang, Jianhua Zhao, Dandan Du, Jindong Qiu, Chong Li
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/11/2459
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author Yirong Yang
Zhenhua Zhao
Bo Dong
Rui Zhang
Jing Jiang
Fengjie Ma
Yingying Zhang
Jianhua Zhao
Dandan Du
Jindong Qiu
Chong Li
author_facet Yirong Yang
Zhenhua Zhao
Bo Dong
Rui Zhang
Jing Jiang
Fengjie Ma
Yingying Zhang
Jianhua Zhao
Dandan Du
Jindong Qiu
Chong Li
author_sort Yirong Yang
collection DOAJ
description Different fertilization measures affect the soil’s physical and chemical properties and bacterial community structure, which in turn affects the growth environment and yield of maize seed production. Therefore, rational fertilization measures are important in maintaining and improving soil fertility and promoting maize crop growth. Research on fertilization practices in maize fields for seed production can help to increase agricultural productivity while protecting and enhancing soil health and achieving sustainable agricultural development. To clarify the effects of different fertilization measures on soil bacterial communities in seed corn fields, 16S rRNA high-throughput sequencing technology and PICRUSt method were used to explore the soil under different fertilization measures (CK as control, effects of single application of liquid organic fertilizer (M), single application of bacterial agents (BF), and combined application of liquid organic fertilizer and bacterial agents (M + BF)) on soil bacterial community structure characteristics and ecological functions. <i>Proteobacteria</i> (20.14–25.30%), <i>Actinobacteriota</i> (18.21–20.47%), <i>Actinobacteriota</i> (13.55–22.00%), and <i>Chloroflexi</i> (14.24–17.59%) were the dominant phyla. <i>Bacillus</i>, <i>RB41</i>, <i>Arthrobacter</i>, and <i>Sphingomonas</i> were the dominant genera. M + BF treatment significantly increased the relative abundance of <i>Planctomycetota</i>. The relative abundance of <i>Bacillus</i> and <i>PaeniBacillus</i> in M treatment decreased considerably, while the relative abundance of <i>Turicibacter</i> increased significantly. The relative abundance of <i>Sphingomonas</i> was reduced considerably in M and M + BF treatments. The relative abundance of <i>Subgroup 10</i> decreased significantly after BF and M + BF treatments. BF treatment significantly increased the relative abundance of <i>Skermanella</i>. Redundancy analysis showed that alkali-hydrolyzed nitrogen (<i>p</i> = 0.044) was the main environmental factor affecting soil bacterial communities under different fertilizer treatments. PICRUSt function prediction results showed that metabolism was the main functional component of bacteria, accounting for 78.45–78.94%. The abundance of functional genes for terpenoid and polyketone metabolism, the endocrine system, the excretory system, and the immune system of the soil bacterial community was significantly increased under M treatment, while the abundance of functional genes for the digestive system was decreased considerably. Different fertilizer cultivation measures changed soil bacterial community composition and ecological function in maize fields. These results can provide a theoretical reference for the study of bacterial community succession characteristics in maize fields and the determination of appropriate fertilizer cultivation measures.
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spelling doaj-art-b31a7de137e44a458a7c7f9a6083fa952025-08-20T02:26:51ZengMDPI AGAgronomy2073-43952024-10-011411245910.3390/agronomy14112459Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn FieldsYirong Yang0Zhenhua Zhao1Bo Dong2Rui Zhang3Jing Jiang4Fengjie Ma5Yingying Zhang6Jianhua Zhao7Dandan Du8Jindong Qiu9Chong Li10Gansu Academy of Agri-Engineering Technology, Lanzhou 730030, ChinaCollege of Resources and Environment, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environment, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Resources and Hydropower, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environment, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Academy of Agri-Engineering Technology, Lanzhou 730030, ChinaGansu Academy of Agri-Engineering Technology, Lanzhou 730030, ChinaDryland Farming Institute, Gansu Academy of Agricultural Sciences, Key Laboratory of Efficient Utilization of Water in Dryland Farming of Gansu Province, Lanzhou 730070, ChinaDryland Farming Institute, Gansu Academy of Agricultural Sciences, Key Laboratory of Efficient Utilization of Water in Dryland Farming of Gansu Province, Lanzhou 730070, ChinaGansu Academy of Agri-Engineering Technology, Lanzhou 730030, ChinaGansu Academy of Agri-Engineering Technology, Lanzhou 730030, ChinaDifferent fertilization measures affect the soil’s physical and chemical properties and bacterial community structure, which in turn affects the growth environment and yield of maize seed production. Therefore, rational fertilization measures are important in maintaining and improving soil fertility and promoting maize crop growth. Research on fertilization practices in maize fields for seed production can help to increase agricultural productivity while protecting and enhancing soil health and achieving sustainable agricultural development. To clarify the effects of different fertilization measures on soil bacterial communities in seed corn fields, 16S rRNA high-throughput sequencing technology and PICRUSt method were used to explore the soil under different fertilization measures (CK as control, effects of single application of liquid organic fertilizer (M), single application of bacterial agents (BF), and combined application of liquid organic fertilizer and bacterial agents (M + BF)) on soil bacterial community structure characteristics and ecological functions. <i>Proteobacteria</i> (20.14–25.30%), <i>Actinobacteriota</i> (18.21–20.47%), <i>Actinobacteriota</i> (13.55–22.00%), and <i>Chloroflexi</i> (14.24–17.59%) were the dominant phyla. <i>Bacillus</i>, <i>RB41</i>, <i>Arthrobacter</i>, and <i>Sphingomonas</i> were the dominant genera. M + BF treatment significantly increased the relative abundance of <i>Planctomycetota</i>. The relative abundance of <i>Bacillus</i> and <i>PaeniBacillus</i> in M treatment decreased considerably, while the relative abundance of <i>Turicibacter</i> increased significantly. The relative abundance of <i>Sphingomonas</i> was reduced considerably in M and M + BF treatments. The relative abundance of <i>Subgroup 10</i> decreased significantly after BF and M + BF treatments. BF treatment significantly increased the relative abundance of <i>Skermanella</i>. Redundancy analysis showed that alkali-hydrolyzed nitrogen (<i>p</i> = 0.044) was the main environmental factor affecting soil bacterial communities under different fertilizer treatments. PICRUSt function prediction results showed that metabolism was the main functional component of bacteria, accounting for 78.45–78.94%. The abundance of functional genes for terpenoid and polyketone metabolism, the endocrine system, the excretory system, and the immune system of the soil bacterial community was significantly increased under M treatment, while the abundance of functional genes for the digestive system was decreased considerably. Different fertilizer cultivation measures changed soil bacterial community composition and ecological function in maize fields. These results can provide a theoretical reference for the study of bacterial community succession characteristics in maize fields and the determination of appropriate fertilizer cultivation measures.https://www.mdpi.com/2073-4395/14/11/2459high-throughput sequencingbacterial community structurePICRUSt functional prediction
spellingShingle Yirong Yang
Zhenhua Zhao
Bo Dong
Rui Zhang
Jing Jiang
Fengjie Ma
Yingying Zhang
Jianhua Zhao
Dandan Du
Jindong Qiu
Chong Li
Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
Agronomy
high-throughput sequencing
bacterial community structure
PICRUSt functional prediction
title Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
title_full Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
title_fullStr Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
title_full_unstemmed Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
title_short Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
title_sort effects of different fertilization measures on bacterial community structure in seed production corn fields
topic high-throughput sequencing
bacterial community structure
PICRUSt functional prediction
url https://www.mdpi.com/2073-4395/14/11/2459
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