Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength
Introduction: Stem cell transplantation is widely employed to treat various diseases, and adipose-derived mesenchymal stem cells (ASCs) are used for allogeneic xenotransplantation. However, muscle function post stem cell transplantation remains largely understudied. Therefore, we aimed to investigat...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320425001312 |
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| author | Tomohiro Abe Emiko Tanaka Isomura Toshie Kuwahara Ryo Mitsui Makoto Matsukawa Kiyoko Nakagawa Susumu Tanaka Yasuhiko Tabata |
| author_facet | Tomohiro Abe Emiko Tanaka Isomura Toshie Kuwahara Ryo Mitsui Makoto Matsukawa Kiyoko Nakagawa Susumu Tanaka Yasuhiko Tabata |
| author_sort | Tomohiro Abe |
| collection | DOAJ |
| description | Introduction: Stem cell transplantation is widely employed to treat various diseases, and adipose-derived mesenchymal stem cells (ASCs) are used for allogeneic xenotransplantation. However, muscle function post stem cell transplantation remains largely understudied. Therefore, we aimed to investigate the optimal conditions for the transplantation of ASC aggregates of gelatin hydrogel microparticles incorporating growth factors. We further aimed to establish a method for improving muscle function via ASC implantation combined with muscle loading through treadmill running. Methods: Mouse ASCs suspended in various solutions were transplanted into the soleus muscles. The strength of each mouse was measured using a digital force gauge after a muscle load was applied using a treadmill. Results: Platelet-rich growth factor-BB (PDGF-BB) effectively facilitated the expression of MYO-D in the ASCs. Moreover, the injection of cell aggregates rather than suspensions enhanced cell retention. Transplantation of ASC-aggregate gelatin hydrogel microparticles incorporating PDGF-BB in combination with muscle load using a treadmill enhanced mouse muscle function. Conclusions: ASC aggregates with growth factor transplantation likely enhance cell retention. Moreover, they likely improve muscle function and load. Thus, our findings provide new avenues for cell regeneration therapy in muscle rehabilitation. |
| format | Article |
| id | doaj-art-b31327d5d25a4a5fa3671bfc9c448c6b |
| institution | Kabale University |
| issn | 2352-3204 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-b31327d5d25a4a5fa3671bfc9c448c6b2025-08-20T03:29:31ZengElsevierRegenerative Therapy2352-32042025-12-013029930810.1016/j.reth.2025.05.016Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strengthTomohiro Abe0Emiko Tanaka Isomura1Toshie Kuwahara2Ryo Mitsui3Makoto Matsukawa4Kiyoko Nakagawa5Susumu Tanaka6Yasuhiko Tabata7Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, The University of Osaka, Osaka, Japan; Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Life and Medical Sciences, Kyoto University, Kyoto, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Dentistry, The University of Osaka, Osaka, JapanLaboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Life and Medical Sciences, Kyoto University, Kyoto, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Dentistry, The University of Osaka, Osaka, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Dentistry, The University of Osaka, Osaka, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Dentistry, The University of Osaka, Osaka, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Dentistry, The University of Osaka, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan; Corresponding author. 53 Kawara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.Introduction: Stem cell transplantation is widely employed to treat various diseases, and adipose-derived mesenchymal stem cells (ASCs) are used for allogeneic xenotransplantation. However, muscle function post stem cell transplantation remains largely understudied. Therefore, we aimed to investigate the optimal conditions for the transplantation of ASC aggregates of gelatin hydrogel microparticles incorporating growth factors. We further aimed to establish a method for improving muscle function via ASC implantation combined with muscle loading through treadmill running. Methods: Mouse ASCs suspended in various solutions were transplanted into the soleus muscles. The strength of each mouse was measured using a digital force gauge after a muscle load was applied using a treadmill. Results: Platelet-rich growth factor-BB (PDGF-BB) effectively facilitated the expression of MYO-D in the ASCs. Moreover, the injection of cell aggregates rather than suspensions enhanced cell retention. Transplantation of ASC-aggregate gelatin hydrogel microparticles incorporating PDGF-BB in combination with muscle load using a treadmill enhanced mouse muscle function. Conclusions: ASC aggregates with growth factor transplantation likely enhance cell retention. Moreover, they likely improve muscle function and load. Thus, our findings provide new avenues for cell regeneration therapy in muscle rehabilitation.http://www.sciencedirect.com/science/article/pii/S2352320425001312Stem cell transplantationAdipose-derived stem cellsCell aggregatesMuscle strength |
| spellingShingle | Tomohiro Abe Emiko Tanaka Isomura Toshie Kuwahara Ryo Mitsui Makoto Matsukawa Kiyoko Nakagawa Susumu Tanaka Yasuhiko Tabata Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength Regenerative Therapy Stem cell transplantation Adipose-derived stem cells Cell aggregates Muscle strength |
| title | Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength |
| title_full | Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength |
| title_fullStr | Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength |
| title_full_unstemmed | Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength |
| title_short | Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength |
| title_sort | transplantation of adipose derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength |
| topic | Stem cell transplantation Adipose-derived stem cells Cell aggregates Muscle strength |
| url | http://www.sciencedirect.com/science/article/pii/S2352320425001312 |
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