Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece
A backyard cat with symptoms of otitis was transferred to a veterinary clinic in Central Greece. A sample was obtained and <i>P. aeruginosa</i> was isolated. The strain exhibited an extensively drug-resistant (XDR) profile, as it was non-susceptible to all tested agents except colistin....
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MDPI AG
2025-06-01
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| Online Access: | https://www.mdpi.com/2306-7381/12/6/576 |
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| author | Marios Lysitsas Eleftherios Triantafillou Irene Chatzipanagiotidou Anastasios Triantafillou Georgia Agorou Maria Eleni Filippitzi Antonis Giakountis George Valiakos |
| author_facet | Marios Lysitsas Eleftherios Triantafillou Irene Chatzipanagiotidou Anastasios Triantafillou Georgia Agorou Maria Eleni Filippitzi Antonis Giakountis George Valiakos |
| author_sort | Marios Lysitsas |
| collection | DOAJ |
| description | A backyard cat with symptoms of otitis was transferred to a veterinary clinic in Central Greece. A sample was obtained and <i>P. aeruginosa</i> was isolated. The strain exhibited an extensively drug-resistant (XDR) profile, as it was non-susceptible to all tested agents except colistin. DNA extraction and whole-genome sequencing (WGS) were performed using a robotic extractor and Ion Torrent technology, respectively. The genome was assembled and screened for resistance and virulence determinants. The isolate belonged to the high-risk clone ST308 with a total of 67 antibiotic resistance genes (ARGs) and 221 virulence factor-related genes being identified. No plasmids were detected. The metallo-beta-lactamase (MBL) <i>bla</i><sub>NDM-1</sub> gene and 46 efflux pumps were included in the strain’s resistome. Both ARGs conferring tolerance to disinfecting agents and biofilm-related genes were identified, associated with the ability of this clone to adapt and persist in healthcare facilities. This case highlights the risk of relevant bacterial clones spreading in the community and even being transmitted to companion animals, causing challenging opportunistic infections to susceptible individuals, while others may become carriers, further spreading the clones to their owners, other animals and the environment. |
| format | Article |
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| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj-art-b30c200ab2734db7af1023e968379bdf2025-08-20T02:21:52ZengMDPI AGVeterinary Sciences2306-73812025-06-0112657610.3390/vetsci12060576Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in GreeceMarios Lysitsas0Eleftherios Triantafillou1Irene Chatzipanagiotidou2Anastasios Triantafillou3Georgia Agorou4Maria Eleni Filippitzi5Antonis Giakountis6George Valiakos7Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, GreeceVet Analyseis, Private Diagnostic Laboratory, 41335 Larissa, GreeceFaculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, GreeceFaculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, GreeceCeMIA SA, 41334 Larissa, GreeceFaculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, GreeceFaculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, GreeceA backyard cat with symptoms of otitis was transferred to a veterinary clinic in Central Greece. A sample was obtained and <i>P. aeruginosa</i> was isolated. The strain exhibited an extensively drug-resistant (XDR) profile, as it was non-susceptible to all tested agents except colistin. DNA extraction and whole-genome sequencing (WGS) were performed using a robotic extractor and Ion Torrent technology, respectively. The genome was assembled and screened for resistance and virulence determinants. The isolate belonged to the high-risk clone ST308 with a total of 67 antibiotic resistance genes (ARGs) and 221 virulence factor-related genes being identified. No plasmids were detected. The metallo-beta-lactamase (MBL) <i>bla</i><sub>NDM-1</sub> gene and 46 efflux pumps were included in the strain’s resistome. Both ARGs conferring tolerance to disinfecting agents and biofilm-related genes were identified, associated with the ability of this clone to adapt and persist in healthcare facilities. This case highlights the risk of relevant bacterial clones spreading in the community and even being transmitted to companion animals, causing challenging opportunistic infections to susceptible individuals, while others may become carriers, further spreading the clones to their owners, other animals and the environment.https://www.mdpi.com/2306-7381/12/6/576<i>bla</i><sub>NDM-1</sub>carbapenemasecat<i>Pseudomonas aeruginosa</i>ST308virulence factors |
| spellingShingle | Marios Lysitsas Eleftherios Triantafillou Irene Chatzipanagiotidou Anastasios Triantafillou Georgia Agorou Maria Eleni Filippitzi Antonis Giakountis George Valiakos Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece Veterinary Sciences <i>bla</i><sub>NDM-1</sub> carbapenemase cat <i>Pseudomonas aeruginosa</i> ST308 virulence factors |
| title | Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece |
| title_full | Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece |
| title_fullStr | Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece |
| title_full_unstemmed | Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece |
| title_short | Companion Animals as Reservoirs of Multidrug Resistance—A Rare Case of an XDR, NDM-1-Producing <i>Pseudomonas aeruginosa</i> Strain of Feline Origin in Greece |
| title_sort | companion animals as reservoirs of multidrug resistance a rare case of an xdr ndm 1 producing i pseudomonas aeruginosa i strain of feline origin in greece |
| topic | <i>bla</i><sub>NDM-1</sub> carbapenemase cat <i>Pseudomonas aeruginosa</i> ST308 virulence factors |
| url | https://www.mdpi.com/2306-7381/12/6/576 |
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