Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells.
UVB radiation causes about 90% of non-melanoma skin cancers by damaging DNA either directly or indirectly by increasing levels of reactive oxygen species (ROS). Skin, chronically exposed to both endogenous and environmental pro-oxidant agents, contains a well-organised system of chemical and enzymat...
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| Format: | Article |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0080728 |
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| author | Nicoletta Vitale Annamaria Kisslinger Simona Paladino Claudio Procaccini Giuseppe Matarese Giovanna Maria Pierantoni Francesco Paolo Mancini Donatella Tramontano |
| author_facet | Nicoletta Vitale Annamaria Kisslinger Simona Paladino Claudio Procaccini Giuseppe Matarese Giovanna Maria Pierantoni Francesco Paolo Mancini Donatella Tramontano |
| author_sort | Nicoletta Vitale |
| collection | DOAJ |
| description | UVB radiation causes about 90% of non-melanoma skin cancers by damaging DNA either directly or indirectly by increasing levels of reactive oxygen species (ROS). Skin, chronically exposed to both endogenous and environmental pro-oxidant agents, contains a well-organised system of chemical and enzymatic antioxidants. However, increased or prolonged free radical action can overwhelm ROS defence mechanisms, contributing to the development of cutaneous diseases. Thus, new strategies for skin protection comprise the use of food antioxidants to counteract oxidative stress. Resveratrol, a phytoalexin from grape, has gained a great interest for its ability to influence several biological mechanisms like redox balance, cell proliferation, signal transduction pathways, immune and inflammatory response. Therefore, the potential of resveratrol to modify skin cell response to UVB exposure could turn out to be a useful option to protect skin from sunlight-induced degenerative diseases. To investigate into this matter, HaCaT cells, a largely used model for human skin keratinocytes, were treated with 25 or 100 µM resveratrol for 2 and 24 hours prior to UVB irradiation (10 to 100 mJ/cm(2)). Cell viability and molecular markers of proliferation, oxidative stress, apoptosis, and autophagy were analyzed. In HaCaT cells resveratrol pretreatment: reduces UVB-induced ROS formation, enhances the detrimental effect of UVB on HaCaT cell vitality, increases UVB-induced caspase 8, PARP cleavage, and induces autophagy. These findings suggest that resveratrol could exert photochemopreventive effects by enhancing UVB-induced apoptosis and by inducing autophagy, thus reducing the odds that damaged cells could escape programmed cell death and initiate malignant transformation. |
| format | Article |
| id | doaj-art-b30bce4e93264644a389cf53cd7ad336 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b30bce4e93264644a389cf53cd7ad3362025-08-20T02:22:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e8072810.1371/journal.pone.0080728Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells.Nicoletta VitaleAnnamaria KisslingerSimona PaladinoClaudio ProcacciniGiuseppe MatareseGiovanna Maria PierantoniFrancesco Paolo ManciniDonatella TramontanoUVB radiation causes about 90% of non-melanoma skin cancers by damaging DNA either directly or indirectly by increasing levels of reactive oxygen species (ROS). Skin, chronically exposed to both endogenous and environmental pro-oxidant agents, contains a well-organised system of chemical and enzymatic antioxidants. However, increased or prolonged free radical action can overwhelm ROS defence mechanisms, contributing to the development of cutaneous diseases. Thus, new strategies for skin protection comprise the use of food antioxidants to counteract oxidative stress. Resveratrol, a phytoalexin from grape, has gained a great interest for its ability to influence several biological mechanisms like redox balance, cell proliferation, signal transduction pathways, immune and inflammatory response. Therefore, the potential of resveratrol to modify skin cell response to UVB exposure could turn out to be a useful option to protect skin from sunlight-induced degenerative diseases. To investigate into this matter, HaCaT cells, a largely used model for human skin keratinocytes, were treated with 25 or 100 µM resveratrol for 2 and 24 hours prior to UVB irradiation (10 to 100 mJ/cm(2)). Cell viability and molecular markers of proliferation, oxidative stress, apoptosis, and autophagy were analyzed. In HaCaT cells resveratrol pretreatment: reduces UVB-induced ROS formation, enhances the detrimental effect of UVB on HaCaT cell vitality, increases UVB-induced caspase 8, PARP cleavage, and induces autophagy. These findings suggest that resveratrol could exert photochemopreventive effects by enhancing UVB-induced apoptosis and by inducing autophagy, thus reducing the odds that damaged cells could escape programmed cell death and initiate malignant transformation.https://doi.org/10.1371/journal.pone.0080728 |
| spellingShingle | Nicoletta Vitale Annamaria Kisslinger Simona Paladino Claudio Procaccini Giuseppe Matarese Giovanna Maria Pierantoni Francesco Paolo Mancini Donatella Tramontano Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. PLoS ONE |
| title | Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. |
| title_full | Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. |
| title_fullStr | Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. |
| title_full_unstemmed | Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. |
| title_short | Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. |
| title_sort | resveratrol couples apoptosis with autophagy in uvb irradiated hacat cells |
| url | https://doi.org/10.1371/journal.pone.0080728 |
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