Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia
Summary: Here, we present a protocol that models peripheral artery disease using the surgical induction of hindlimb ischemia (HLI) in mice. We list the steps to surgically excise the femoral artery and assess blood flow recovery through laser Doppler imaging. We then detail procedures for visualizin...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001893 |
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| author | Sheila Sharma Julia Pierce Celia A. Butler Olivya Caballero Chris Mantsounga Alan R. Morrison |
| author_facet | Sheila Sharma Julia Pierce Celia A. Butler Olivya Caballero Chris Mantsounga Alan R. Morrison |
| author_sort | Sheila Sharma |
| collection | DOAJ |
| description | Summary: Here, we present a protocol that models peripheral artery disease using the surgical induction of hindlimb ischemia (HLI) in mice. We list the steps to surgically excise the femoral artery and assess blood flow recovery through laser Doppler imaging. We then detail procedures for visualizing immune cell recruitment and blood vessel growth to explore the role of inflammatory macrophages in angio/arteriogenesis.For complete details on the use and execution of this protocol, please refer to Mantsounga et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-b30b322074f04468a145ae2f7db6dab7 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-b30b322074f04468a145ae2f7db6dab72025-08-20T02:14:43ZengElsevierSTAR Protocols2666-16672025-06-016210378310.1016/j.xpro.2025.103783Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemiaSheila Sharma0Julia Pierce1Celia A. Butler2Olivya Caballero3Chris Mantsounga4Alan R. Morrison5Vascular Research Laboratory, Providence VA Medical Center, Research (151), 830 Chalkstone Avenue, Providence, RI 02908, USA; Ocean State Research Institute, Inc., 830 Chalkstone Avenue, Providence, RI 02908, USA; Department of Medicine, Alpert Medical School of Brown University, Providence, RI 02903, USAVascular Research Laboratory, Providence VA Medical Center, Research (151), 830 Chalkstone Avenue, Providence, RI 02908, USA; Ocean State Research Institute, Inc., 830 Chalkstone Avenue, Providence, RI 02908, USA; Department of Medicine, Alpert Medical School of Brown University, Providence, RI 02903, USAVascular Research Laboratory, Providence VA Medical Center, Research (151), 830 Chalkstone Avenue, Providence, RI 02908, USA; Ocean State Research Institute, Inc., 830 Chalkstone Avenue, Providence, RI 02908, USA; Department of Medicine, Alpert Medical School of Brown University, Providence, RI 02903, USAVascular Research Laboratory, Providence VA Medical Center, Research (151), 830 Chalkstone Avenue, Providence, RI 02908, USA; Ocean State Research Institute, Inc., 830 Chalkstone Avenue, Providence, RI 02908, USA; Department of Medicine, Alpert Medical School of Brown University, Providence, RI 02903, USAVascular Research Laboratory, Providence VA Medical Center, Research (151), 830 Chalkstone Avenue, Providence, RI 02908, USA; Ocean State Research Institute, Inc., 830 Chalkstone Avenue, Providence, RI 02908, USA; Department of Medicine, Alpert Medical School of Brown University, Providence, RI 02903, USA; Corresponding authorVascular Research Laboratory, Providence VA Medical Center, Research (151), 830 Chalkstone Avenue, Providence, RI 02908, USA; Ocean State Research Institute, Inc., 830 Chalkstone Avenue, Providence, RI 02908, USA; Department of Medicine, Alpert Medical School of Brown University, Providence, RI 02903, USA; Corresponding authorSummary: Here, we present a protocol that models peripheral artery disease using the surgical induction of hindlimb ischemia (HLI) in mice. We list the steps to surgically excise the femoral artery and assess blood flow recovery through laser Doppler imaging. We then detail procedures for visualizing immune cell recruitment and blood vessel growth to explore the role of inflammatory macrophages in angio/arteriogenesis.For complete details on the use and execution of this protocol, please refer to Mantsounga et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001893Health SciencesImmunologyMicroscopyModel OrganismsMolecular Biology |
| spellingShingle | Sheila Sharma Julia Pierce Celia A. Butler Olivya Caballero Chris Mantsounga Alan R. Morrison Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia STAR Protocols Health Sciences Immunology Microscopy Model Organisms Molecular Biology |
| title | Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia |
| title_full | Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia |
| title_fullStr | Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia |
| title_full_unstemmed | Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia |
| title_short | Protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia |
| title_sort | protocol for modeling blood flow recovery and angiogenesis in response to hindlimb ischemia |
| topic | Health Sciences Immunology Microscopy Model Organisms Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001893 |
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