Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology
Kai-Xin Zhang,1 Ji-Wei Zhang,2 Yan-Hong Jiang,1 Yi-Ran Wang,1 Zhen-Ling Liu,1 Peng-Li Ding,1 Xiang-Ying Wang,1 Wen-Qiang Cui,3 Xiang-Qing Xu,3,* Ya-Han Wang3,* 1First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic o...
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Dove Medical Press
2025-02-01
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| Series: | Drug Design, Development and Therapy |
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| author | Zhang KX Zhang JW Jiang YH Wang YR Liu ZL Ding PL Wang XY Cui WQ Xu XQ Wang YH |
| author_facet | Zhang KX Zhang JW Jiang YH Wang YR Liu ZL Ding PL Wang XY Cui WQ Xu XQ Wang YH |
| author_sort | Zhang KX |
| collection | DOAJ |
| description | Kai-Xin Zhang,1 Ji-Wei Zhang,2 Yan-Hong Jiang,1 Yi-Ran Wang,1 Zhen-Ling Liu,1 Peng-Li Ding,1 Xiang-Ying Wang,1 Wen-Qiang Cui,3 Xiang-Qing Xu,3,* Ya-Han Wang3,* 1First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China; 2College of Acupuncture and Massage, Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China; 3Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiang-Qing Xu, Ya-Han Wang; Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China, Email happyxiangqing@163.com; sdwangyahan@sina.comBackground: Danggui-Shaoyao-San (DSS) is a traditional Chinese medicine prescription with a history of nearly 2000 years, originally widely used for gynecological diseases, and in recent years research has found that DSS also has a good therapeutic effect on Alzheimer’s disease (AD).Purpose: The objective is to investigate the metabolic components of the DSS in the blood and the potential mechanisms for AD.Materials and Methods: Liquid chromatography‒mass spectrometry (LC-MS) combined with gas chromatography‒mass spectrometry (GC-MS) based non-targeted metabolomics were used to conduct in-depth research. Serum Pharmacology was used to analyze potential mechanisms of DSS for AD. C57BL/6J mice and Hippocampal neuronal cell line (HT-22) were used to prepare the AD model. Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. Flow cytometry and Cell Counting Kit-8 (CCK-8) reveal the effect of DSS serum on HT-22 proliferation and apoptosis.Results: A total of 57 metabolic components were screened in DSS serum. Serum Pharmacology revealed that the calcium signaling pathway and cAMP/PKA/CREB pathway may be a potential mechanism through which DSS treated AD. DSS can reduce aberrant phosphorylation of Tau and modulates cAMP/PKA/CREB pathway to improve cognition and apoptosis in AD mice. DSS serum can increase the cell viability of HT-22 and reduce apoptosis mainly by alleviating mitochondrial calcium overloading.Conclusion: DSS can modulate the calcium signaling pathway and enhance the cAMP/PKA/CREB signaling pathway to ameliorate Tau aberrant phosphorylation, cognitive deficits and neuronal apoptosis after AD. Keywords: Alzheimer’s disease, calcium signaling pathway, cAMP/PKA/CREB signaling pathway, Danggui-Shaoyao-San, apoptosis, serum pharmacology |
| format | Article |
| id | doaj-art-b308b5d662e84f2c827e938e338ac746 |
| institution | Kabale University |
| issn | 1177-8881 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
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| series | Drug Design, Development and Therapy |
| spelling | doaj-art-b308b5d662e84f2c827e938e338ac7462025-02-11T17:30:55ZengDove Medical PressDrug Design, Development and Therapy1177-88812025-02-01Volume 19911929100002Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum PharmacologyZhang KXZhang JWJiang YHWang YRLiu ZLDing PLWang XYCui WQXu XQWang YHKai-Xin Zhang,1 Ji-Wei Zhang,2 Yan-Hong Jiang,1 Yi-Ran Wang,1 Zhen-Ling Liu,1 Peng-Li Ding,1 Xiang-Ying Wang,1 Wen-Qiang Cui,3 Xiang-Qing Xu,3,* Ya-Han Wang3,* 1First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China; 2College of Acupuncture and Massage, Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China; 3Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiang-Qing Xu, Ya-Han Wang; Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250000, People’s Republic of China, Email happyxiangqing@163.com; sdwangyahan@sina.comBackground: Danggui-Shaoyao-San (DSS) is a traditional Chinese medicine prescription with a history of nearly 2000 years, originally widely used for gynecological diseases, and in recent years research has found that DSS also has a good therapeutic effect on Alzheimer’s disease (AD).Purpose: The objective is to investigate the metabolic components of the DSS in the blood and the potential mechanisms for AD.Materials and Methods: Liquid chromatography‒mass spectrometry (LC-MS) combined with gas chromatography‒mass spectrometry (GC-MS) based non-targeted metabolomics were used to conduct in-depth research. Serum Pharmacology was used to analyze potential mechanisms of DSS for AD. C57BL/6J mice and Hippocampal neuronal cell line (HT-22) were used to prepare the AD model. Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. Flow cytometry and Cell Counting Kit-8 (CCK-8) reveal the effect of DSS serum on HT-22 proliferation and apoptosis.Results: A total of 57 metabolic components were screened in DSS serum. Serum Pharmacology revealed that the calcium signaling pathway and cAMP/PKA/CREB pathway may be a potential mechanism through which DSS treated AD. DSS can reduce aberrant phosphorylation of Tau and modulates cAMP/PKA/CREB pathway to improve cognition and apoptosis in AD mice. DSS serum can increase the cell viability of HT-22 and reduce apoptosis mainly by alleviating mitochondrial calcium overloading.Conclusion: DSS can modulate the calcium signaling pathway and enhance the cAMP/PKA/CREB signaling pathway to ameliorate Tau aberrant phosphorylation, cognitive deficits and neuronal apoptosis after AD. Keywords: Alzheimer’s disease, calcium signaling pathway, cAMP/PKA/CREB signaling pathway, Danggui-Shaoyao-San, apoptosis, serum pharmacologyhttps://www.dovepress.com/danggui-shaoyao-san-can-ameliorate-alzheimers-disease-by-inhibiting-hi-peer-reviewed-fulltext-article-DDDTalzheimer's diseasecalcium signaling pathwaycamp/pka/creb signaling pathwaydanggui-shaoyao-sanapoptosisserum pharmacology |
| spellingShingle | Zhang KX Zhang JW Jiang YH Wang YR Liu ZL Ding PL Wang XY Cui WQ Xu XQ Wang YH Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology Drug Design, Development and Therapy alzheimer's disease calcium signaling pathway camp/pka/creb signaling pathway danggui-shaoyao-san apoptosis serum pharmacology |
| title | Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology |
| title_full | Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology |
| title_fullStr | Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology |
| title_full_unstemmed | Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology |
| title_short | Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology |
| title_sort | danggui shaoyao san can ameliorate alzheimer rsquo s disease by inhibiting hippocampal neuron apoptosis findings from serum pharmacology |
| topic | alzheimer's disease calcium signaling pathway camp/pka/creb signaling pathway danggui-shaoyao-san apoptosis serum pharmacology |
| url | https://www.dovepress.com/danggui-shaoyao-san-can-ameliorate-alzheimers-disease-by-inhibiting-hi-peer-reviewed-fulltext-article-DDDT |
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