Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer
ABSTRACT Background Existing studies have shown that transferrin receptor (TfR) is highly expressed on the surface of lung cancer cells, and nanoparticles (NPs) have been widely used as delivery vehicles. The aim of this study was to investigate the effect of the targeted delivery of Fe3O4 NPs modif...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Thoracic Cancer |
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| Online Access: | https://doi.org/10.1111/1759-7714.15497 |
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| author | Xiaoxu Lan Xiao Wang Liying Shao Jiayue An Simin Rong Xiancong Yang Hongfang Sun Yan Liang Ranran Wang Shuyang Xie Youjie Li |
| author_facet | Xiaoxu Lan Xiao Wang Liying Shao Jiayue An Simin Rong Xiancong Yang Hongfang Sun Yan Liang Ranran Wang Shuyang Xie Youjie Li |
| author_sort | Xiaoxu Lan |
| collection | DOAJ |
| description | ABSTRACT Background Existing studies have shown that transferrin receptor (TfR) is highly expressed on the surface of lung cancer cells, and nanoparticles (NPs) have been widely used as delivery vehicles. The aim of this study was to investigate the effect of the targeted delivery of Fe3O4 NPs modified with transferrin (Tf) compared with photothermal treatment for lung cancer. Methods The morphology and properties of Fe3O4 NPs modified with Tf were tested by internal morphological characterization experiments including transmission electron microscopy, particle size meter infrared spectrometer and other experiments. The delivery of materials was investigated by cell proliferation and apoptosis experiments, and western blot experiment was used to detect yes‐associated protein 1(YAP1) protein expression changes after delivering miR‐15a‐5p. In addition, animal models were constructed to further explore the targeting properties of the material. Results The results demonstrated that the nanomaterial has good stability and targeting properties. Meanwhile, we also discovered that the miR‐15a‐5p carried by NPs can inhibit cell growth after its entry to the lung cancer cells. The effect became more evident when the nanomaterials were assisted with laser therapy, as verified by in vivo and in vitro experiments. In terms of the related mechanism, miR‐15a‐5p inhibited YAP1 expression, which affected cell proliferation and apoptosis. Conclusion In this study, Fe3O4 NPs modified with Tf delivered miR‐15a‐5p in combination with photothermal therapy for lung cancer. In future research, the targeted delivery of Tf and the photothermal synergy of nanomaterials will provide a theoretical basis for cancer treatment. |
| format | Article |
| id | doaj-art-b2ee1011f78a4774bf4b82fbe6e590ab |
| institution | Kabale University |
| issn | 1759-7706 1759-7714 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj-art-b2ee1011f78a4774bf4b82fbe6e590ab2025-01-15T16:00:32ZengWileyThoracic Cancer1759-77061759-77142025-01-01161n/an/a10.1111/1759-7714.15497Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung CancerXiaoxu Lan0Xiao Wang1Liying Shao2Jiayue An3Simin Rong4Xiancong Yang5Hongfang Sun6Yan Liang7Ranran Wang8Shuyang Xie9Youjie Li10Department of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaCollege of Biological and Chemical Engineering, Qilu Institute of Technology Jinan Shandong People's Republic of ChinaCentral Laboratory Weihai Municipal Hospital Weihai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaInstitute of Rehabilitation Medicine, School of Rehabilitation Medicine, Binzhou Medical University Yantai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai Shandong People's Republic of ChinaABSTRACT Background Existing studies have shown that transferrin receptor (TfR) is highly expressed on the surface of lung cancer cells, and nanoparticles (NPs) have been widely used as delivery vehicles. The aim of this study was to investigate the effect of the targeted delivery of Fe3O4 NPs modified with transferrin (Tf) compared with photothermal treatment for lung cancer. Methods The morphology and properties of Fe3O4 NPs modified with Tf were tested by internal morphological characterization experiments including transmission electron microscopy, particle size meter infrared spectrometer and other experiments. The delivery of materials was investigated by cell proliferation and apoptosis experiments, and western blot experiment was used to detect yes‐associated protein 1(YAP1) protein expression changes after delivering miR‐15a‐5p. In addition, animal models were constructed to further explore the targeting properties of the material. Results The results demonstrated that the nanomaterial has good stability and targeting properties. Meanwhile, we also discovered that the miR‐15a‐5p carried by NPs can inhibit cell growth after its entry to the lung cancer cells. The effect became more evident when the nanomaterials were assisted with laser therapy, as verified by in vivo and in vitro experiments. In terms of the related mechanism, miR‐15a‐5p inhibited YAP1 expression, which affected cell proliferation and apoptosis. Conclusion In this study, Fe3O4 NPs modified with Tf delivered miR‐15a‐5p in combination with photothermal therapy for lung cancer. In future research, the targeted delivery of Tf and the photothermal synergy of nanomaterials will provide a theoretical basis for cancer treatment.https://doi.org/10.1111/1759-7714.15497Fe3O4 nanoparticleslung cancerphotothermal therapytargeted deliverytransferrin |
| spellingShingle | Xiaoxu Lan Xiao Wang Liying Shao Jiayue An Simin Rong Xiancong Yang Hongfang Sun Yan Liang Ranran Wang Shuyang Xie Youjie Li Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer Thoracic Cancer Fe3O4 nanoparticles lung cancer photothermal therapy targeted delivery transferrin |
| title | Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer |
| title_full | Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer |
| title_fullStr | Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer |
| title_full_unstemmed | Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer |
| title_short | Effect of Transferrin‐Modified Fe3O4 Nanoparticle Targeted Delivery miR‐15a‐5p Combined With Photothermal Therapy on Lung Cancer |
| title_sort | effect of transferrin modified fe3o4 nanoparticle targeted delivery mir 15a 5p combined with photothermal therapy on lung cancer |
| topic | Fe3O4 nanoparticles lung cancer photothermal therapy targeted delivery transferrin |
| url | https://doi.org/10.1111/1759-7714.15497 |
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