Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia
The immune microenvironment plays an important role in leukemia treatment. However, a specific single-cell profiling of the immune alteration in bone marrow of chronic myeloid leukemia (CML) patients is still lacking. We performed multi-level single-cell sequencing to systematically decipher the bon...
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KeAi Communications Co., Ltd.
2025-09-01
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| Series: | Genes and Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352304225001151 |
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| author | Chenjian Zhuo Xin Dong Xueya Zhao Weiru Wu Hao Zhou Jing Feng Lingbo Liu Mingqian Feng Chunjiang He Yu Hou |
| author_facet | Chenjian Zhuo Xin Dong Xueya Zhao Weiru Wu Hao Zhou Jing Feng Lingbo Liu Mingqian Feng Chunjiang He Yu Hou |
| author_sort | Chenjian Zhuo |
| collection | DOAJ |
| description | The immune microenvironment plays an important role in leukemia treatment. However, a specific single-cell profiling of the immune alteration in bone marrow of chronic myeloid leukemia (CML) patients is still lacking. We performed multi-level single-cell sequencing to systematically decipher the bone marrow T cell atlas of CML patients. The results exhibited extensive changes of T cells, including the decreased CD4 T cells and increased CD8 T cells in the CML bone marrow. Subpopulation analysis revealed a significant increase of CD8 terminal effector (TE) cells and a significant decrease of CD4 naïve T cells. T cell receptor sequencing showed that the overall diversity of the T cell receptor repertoire was reduced in CML, with the exception of the CD8 TE cell. In addition, CD8 TE cells were the main source of gene expression differences in CD8 T cells. Intercellular communication analysis revealed the altered interaction between CD8 TE and other non-T cells in CML, including neutrophil subtype, indicating the potential regulation of bone marrow microenvironment cells on CD8 TE dynamics. Collectively, our work characterises the alteration of T cell subsets in CML patients at multiple single-cell levels, providing a valuable resource for understanding the immune microenvironment and developing new immune strategies for CML therapy. |
| format | Article |
| id | doaj-art-b2e780d25faf4e849cc3eef828a6e47c |
| institution | Kabale University |
| issn | 2352-3042 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Genes and Diseases |
| spelling | doaj-art-b2e780d25faf4e849cc3eef828a6e47c2025-08-20T03:29:15ZengKeAi Communications Co., Ltd.Genes and Diseases2352-30422025-09-0112510162610.1016/j.gendis.2025.101626Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemiaChenjian Zhuo0Xin Dong1Xueya Zhao2Weiru Wu3Hao Zhou4Jing Feng5Lingbo Liu6Mingqian Feng7Chunjiang He8Yu Hou9College of Biomedicine and Health, Hubei Hongshan Laboratory, College of Informatics, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaSchool of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, ChinaSchool of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, ChinaDepartment of Clinical Hematology, Third Military Medical University (Army Medical University), Chongqing 400016, ChinaDepartment of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430070, ChinaSchool of Computer Science, Wuhan University, Wuhan, Hubei 430072, ChinaDepartment of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430070, ChinaCollege of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaCollege of Biomedicine and Health, Hubei Hongshan Laboratory, College of Informatics, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Corresponding author.School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China; Corresponding author.The immune microenvironment plays an important role in leukemia treatment. However, a specific single-cell profiling of the immune alteration in bone marrow of chronic myeloid leukemia (CML) patients is still lacking. We performed multi-level single-cell sequencing to systematically decipher the bone marrow T cell atlas of CML patients. The results exhibited extensive changes of T cells, including the decreased CD4 T cells and increased CD8 T cells in the CML bone marrow. Subpopulation analysis revealed a significant increase of CD8 terminal effector (TE) cells and a significant decrease of CD4 naïve T cells. T cell receptor sequencing showed that the overall diversity of the T cell receptor repertoire was reduced in CML, with the exception of the CD8 TE cell. In addition, CD8 TE cells were the main source of gene expression differences in CD8 T cells. Intercellular communication analysis revealed the altered interaction between CD8 TE and other non-T cells in CML, including neutrophil subtype, indicating the potential regulation of bone marrow microenvironment cells on CD8 TE dynamics. Collectively, our work characterises the alteration of T cell subsets in CML patients at multiple single-cell levels, providing a valuable resource for understanding the immune microenvironment and developing new immune strategies for CML therapy.http://www.sciencedirect.com/science/article/pii/S2352304225001151Chronic myeloid leukemiaT cell receptorSingle-cell sequencingT cellCell-cell communication |
| spellingShingle | Chenjian Zhuo Xin Dong Xueya Zhao Weiru Wu Hao Zhou Jing Feng Lingbo Liu Mingqian Feng Chunjiang He Yu Hou Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia Genes and Diseases Chronic myeloid leukemia T cell receptor Single-cell sequencing T cell Cell-cell communication |
| title | Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia |
| title_full | Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia |
| title_fullStr | Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia |
| title_full_unstemmed | Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia |
| title_short | Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia |
| title_sort | single cell sequencing reveals the expansion and diversity of t cell subsets in the bone marrow microenvironment of chronic myeloid leukemia |
| topic | Chronic myeloid leukemia T cell receptor Single-cell sequencing T cell Cell-cell communication |
| url | http://www.sciencedirect.com/science/article/pii/S2352304225001151 |
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