Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs
The melanoma-associated antigen gene (MAGE) is a key target in cancer immunotherapy. Given the potential of MAGE-B genes in veterinary immunotherapy for canine mammary tumors (CMTs), this study investigated the mRNA expression of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i...
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MDPI AG
2025-03-01
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| author | Wanwisa Srisawat Pongpisid Koonyosying Anucha Muenthaisong Kanokwan Sangkakam Thanya Varinrak Nattawooti Sthitmatee |
| author_facet | Wanwisa Srisawat Pongpisid Koonyosying Anucha Muenthaisong Kanokwan Sangkakam Thanya Varinrak Nattawooti Sthitmatee |
| author_sort | Wanwisa Srisawat |
| collection | DOAJ |
| description | The melanoma-associated antigen gene (MAGE) is a key target in cancer immunotherapy. Given the potential of MAGE-B genes in veterinary immunotherapy for canine mammary tumors (CMTs), this study investigated the mRNA expression of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> in CMT tissues and cells from dogs. Quantitative real-time PCR was used to analyze 28 CMT tissue samples, including 4 benign and 24 malignant tumors (13 simple carcinomas, 6 complex carcinomas, 3 carcinosarcomas, and 2 fibrosarcomas). Benign mixed tumor and complex carcinoma-type CMT cells were cultured and treated with a DNA methylase inhibitor (5-aza-2′-deoxycytidine; 5-aza-CdR) and a histone deacetylase inhibitor (Trichostatin A; TSA) under the following four conditions: (1) 5-aza-CdR for 72 h; (2) TSA for 24 h; (3) 5-aza-CdR for 48 h followed by TSA for 24 h; and (4) control. <i>MAGE-B1</i> and <i>-B4</i> showed the highest expression in the CMT samples (100% and 89.29%, respectively), followed by <i>MAGE-B10</i> (82.14%). Carcinosarcomas and simple anaplastic carcinomas had significantly higher MAGE-B expression levels than simple tubulopapillary carcinomas (<i>p</i> < 0.05). 5-aza-CdR treatment increased MAGE-B expression, whereas TSA had a mild effect. Further research involving larger cohorts is needed to confirm these findings. |
| format | Article |
| id | doaj-art-b2e0a14bc6cd40799766426017fb9ae9 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-b2e0a14bc6cd40799766426017fb9ae92025-08-20T03:08:43ZengMDPI AGAnimals2076-26152025-03-0115791010.3390/ani15070910Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in DogsWanwisa Srisawat0Pongpisid Koonyosying1Anucha Muenthaisong2Kanokwan Sangkakam3Thanya Varinrak4Nattawooti Sthitmatee5Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandThe melanoma-associated antigen gene (MAGE) is a key target in cancer immunotherapy. Given the potential of MAGE-B genes in veterinary immunotherapy for canine mammary tumors (CMTs), this study investigated the mRNA expression of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> in CMT tissues and cells from dogs. Quantitative real-time PCR was used to analyze 28 CMT tissue samples, including 4 benign and 24 malignant tumors (13 simple carcinomas, 6 complex carcinomas, 3 carcinosarcomas, and 2 fibrosarcomas). Benign mixed tumor and complex carcinoma-type CMT cells were cultured and treated with a DNA methylase inhibitor (5-aza-2′-deoxycytidine; 5-aza-CdR) and a histone deacetylase inhibitor (Trichostatin A; TSA) under the following four conditions: (1) 5-aza-CdR for 72 h; (2) TSA for 24 h; (3) 5-aza-CdR for 48 h followed by TSA for 24 h; and (4) control. <i>MAGE-B1</i> and <i>-B4</i> showed the highest expression in the CMT samples (100% and 89.29%, respectively), followed by <i>MAGE-B10</i> (82.14%). Carcinosarcomas and simple anaplastic carcinomas had significantly higher MAGE-B expression levels than simple tubulopapillary carcinomas (<i>p</i> < 0.05). 5-aza-CdR treatment increased MAGE-B expression, whereas TSA had a mild effect. Further research involving larger cohorts is needed to confirm these findings.https://www.mdpi.com/2076-2615/15/7/910canine mammary tumorsmelanoma-associated antigen-BmRNA expressionquantitative real-time PCR |
| spellingShingle | Wanwisa Srisawat Pongpisid Koonyosying Anucha Muenthaisong Kanokwan Sangkakam Thanya Varinrak Nattawooti Sthitmatee Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs Animals canine mammary tumors melanoma-associated antigen-B mRNA expression quantitative real-time PCR |
| title | Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs |
| title_full | Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs |
| title_fullStr | Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs |
| title_full_unstemmed | Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs |
| title_short | Preliminary Exploration of <i>MAGE-B1</i>, <i>-B4</i>, <i>-B5</i>, and <i>-B10</i> mRNA Expression in Canine Mammary Tumors in Dogs |
| title_sort | preliminary exploration of i mage b1 i i b4 i i b5 i and i b10 i mrna expression in canine mammary tumors in dogs |
| topic | canine mammary tumors melanoma-associated antigen-B mRNA expression quantitative real-time PCR |
| url | https://www.mdpi.com/2076-2615/15/7/910 |
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