CAR-T cell therapy for cancer: current challenges and future directions

Abstract Chimeric antigen receptor T (CAR-T) cell therapies have transformed the treatment of relapsed/refractory (R/R) B-cell malignancies and multiple myeloma by redirecting activated T cells to CD19- or BCMA-expressing tumor cells. However, this approach has yet to be approved for acute myeloid l...

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Main Authors: Inés Zugasti, Lady Espinosa-Aroca, Klaudyna Fidyt, Vladimir Mulens-Arias, Marina Diaz-Beya, Manel Juan, Álvaro Urbano-Ispizua, Jordi Esteve, Talia Velasco-Hernandez, Pablo Menéndez
Format: Article
Language:English
Published: Nature Publishing Group 2025-07-01
Series:Signal Transduction and Targeted Therapy
Online Access:https://doi.org/10.1038/s41392-025-02269-w
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author Inés Zugasti
Lady Espinosa-Aroca
Klaudyna Fidyt
Vladimir Mulens-Arias
Marina Diaz-Beya
Manel Juan
Álvaro Urbano-Ispizua
Jordi Esteve
Talia Velasco-Hernandez
Pablo Menéndez
author_facet Inés Zugasti
Lady Espinosa-Aroca
Klaudyna Fidyt
Vladimir Mulens-Arias
Marina Diaz-Beya
Manel Juan
Álvaro Urbano-Ispizua
Jordi Esteve
Talia Velasco-Hernandez
Pablo Menéndez
author_sort Inés Zugasti
collection DOAJ
description Abstract Chimeric antigen receptor T (CAR-T) cell therapies have transformed the treatment of relapsed/refractory (R/R) B-cell malignancies and multiple myeloma by redirecting activated T cells to CD19- or BCMA-expressing tumor cells. However, this approach has yet to be approved for acute myeloid leukemia (AML), the most common acute leukemia in adults and the elderly. Simultaneously, CAR-T cell therapies continue to face significant challenges in the treatment of solid tumors. The primary challenge in developing CAR-T cell therapies for AML is the absence of an ideal target antigen that is both effective and safe, as AML cells share most surface antigens with healthy hematopoietic stem and progenitor cells (HSPCs). Simultaneously targeting antigen expression on both AML cells and HSPCs may result in life-threatening on-target/off-tumor toxicities such as prolonged myeloablation. In addition, the immunosuppressive nature of the AML tumor microenvironment has a detrimental effect on the immune response. This review begins with a comprehensive overview of CAR-T cell therapy for cancer, covering the structure of CAR-T cells and the history of their clinical application. It then explores the current landscape of CAR-T cell therapy in both hematologic malignancies and solid tumors. Finally, the review delves into the specific challenges of applying CAR-T cell therapy to AML, highlights ongoing global clinical trials, and outlines potential future directions for developing effective CAR-T cell-based treatments for relapsed/refractory AML.
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spelling doaj-art-b2dc49cba1534357a54bb2f44bcde9322025-08-20T03:45:41ZengNature Publishing GroupSignal Transduction and Targeted Therapy2059-36352025-07-0110115110.1038/s41392-025-02269-wCAR-T cell therapy for cancer: current challenges and future directionsInés Zugasti0Lady Espinosa-Aroca1Klaudyna Fidyt2Vladimir Mulens-Arias3Marina Diaz-Beya4Manel Juan5Álvaro Urbano-Ispizua6Jordi Esteve7Talia Velasco-Hernandez8Pablo Menéndez9Hematology Department, Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)Josep Carreras Leukemia Research InstituteJosep Carreras Leukemia Research InstituteJosep Carreras Leukemia Research InstituteHematology Department, Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)Red Española de Terapias Avanzadas (TERAV), Instituto de Salud Carlos IIIHematology Department, Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)Hematology Department, Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)Josep Carreras Leukemia Research InstituteJosep Carreras Leukemia Research InstituteAbstract Chimeric antigen receptor T (CAR-T) cell therapies have transformed the treatment of relapsed/refractory (R/R) B-cell malignancies and multiple myeloma by redirecting activated T cells to CD19- or BCMA-expressing tumor cells. However, this approach has yet to be approved for acute myeloid leukemia (AML), the most common acute leukemia in adults and the elderly. Simultaneously, CAR-T cell therapies continue to face significant challenges in the treatment of solid tumors. The primary challenge in developing CAR-T cell therapies for AML is the absence of an ideal target antigen that is both effective and safe, as AML cells share most surface antigens with healthy hematopoietic stem and progenitor cells (HSPCs). Simultaneously targeting antigen expression on both AML cells and HSPCs may result in life-threatening on-target/off-tumor toxicities such as prolonged myeloablation. In addition, the immunosuppressive nature of the AML tumor microenvironment has a detrimental effect on the immune response. This review begins with a comprehensive overview of CAR-T cell therapy for cancer, covering the structure of CAR-T cells and the history of their clinical application. It then explores the current landscape of CAR-T cell therapy in both hematologic malignancies and solid tumors. Finally, the review delves into the specific challenges of applying CAR-T cell therapy to AML, highlights ongoing global clinical trials, and outlines potential future directions for developing effective CAR-T cell-based treatments for relapsed/refractory AML.https://doi.org/10.1038/s41392-025-02269-w
spellingShingle Inés Zugasti
Lady Espinosa-Aroca
Klaudyna Fidyt
Vladimir Mulens-Arias
Marina Diaz-Beya
Manel Juan
Álvaro Urbano-Ispizua
Jordi Esteve
Talia Velasco-Hernandez
Pablo Menéndez
CAR-T cell therapy for cancer: current challenges and future directions
Signal Transduction and Targeted Therapy
title CAR-T cell therapy for cancer: current challenges and future directions
title_full CAR-T cell therapy for cancer: current challenges and future directions
title_fullStr CAR-T cell therapy for cancer: current challenges and future directions
title_full_unstemmed CAR-T cell therapy for cancer: current challenges and future directions
title_short CAR-T cell therapy for cancer: current challenges and future directions
title_sort car t cell therapy for cancer current challenges and future directions
url https://doi.org/10.1038/s41392-025-02269-w
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