Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation.
<h4>Background</h4>Innovative treatment strategies are required for stomach ulcers because of their multifactorial nature. Nanotechnology has emerged as a promising and transformative platform for the formulation and targeted delivery of therapeutic agents.<h4>Methods and findings&...
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Public Library of Science (PLoS)
2025-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0327368 |
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| author | Tamer I M Ragab Abdulsalam M Kassem Heba M I Abdallah Sally A El Awdan Naglaa M Ammar Abd El-Nasser G El Gendy Basma Emad Aboulhoda Sherif M Afifi Tuba Esatbeyoglu Enayat A Omara Abdelsamed I Elshamy |
| author_facet | Tamer I M Ragab Abdulsalam M Kassem Heba M I Abdallah Sally A El Awdan Naglaa M Ammar Abd El-Nasser G El Gendy Basma Emad Aboulhoda Sherif M Afifi Tuba Esatbeyoglu Enayat A Omara Abdelsamed I Elshamy |
| author_sort | Tamer I M Ragab |
| collection | DOAJ |
| description | <h4>Background</h4>Innovative treatment strategies are required for stomach ulcers because of their multifactorial nature. Nanotechnology has emerged as a promising and transformative platform for the formulation and targeted delivery of therapeutic agents.<h4>Methods and findings</h4>The gastroprotective potential of both in its free form and encapsulated in calcium alginate beads was evaluated against ethanol-induced gastric ulceration in rats. Phytol-loaded nanoemulsions were incorporated into alginate beads to achieve controlled release. Alginate beads showed a pH-dependent release pattern. The release behavior showed a higher release rate at pH 6.8 than at pH 1.2. Phytol release kinetics followed the Korsmeyer-Peppas model, indicating a release mechanism governed by diffusion and polymer relaxation. Rats were pretreated with Phytol and/or nano-Phytol at 10 or 20 mg/kg doses administered one hour before ethanol exposure. Gastric ulcer was induced by administration of EtOH (1 mL/kg, p.o.) 0.5 h after NG-nitro-L-Arginine Methyl Ester (L-NAME) or Aminoguanidine (AMG) injection. Phytol treatment led to a reduction in ulcer index and severity and improved stomach gross morphology. Also, interleukin-6 (IL-6) gastric contents were reduced, whereas transforming growth factor β (TGF-β1) was elevated, and histopathological features were ameliorated. Western blot analysis revealed that nano-Phytol exerted greater inhibitory effects on caspase-3 and Nuclear Factor kappa B (NF-κB) than unformulated Phytol. Interestingly, Phytol's pharmacological effects on ulcers were enhanced by its nanoformulation in a dose-dependent way without exhibiting any toxicity symptoms. Confocal laser scanning microscopy (CLSM) confirmed significantly improved tissue penetration of nano-Phytol within the stomach layers compared to the Phytol. The Phytol or nano-Phytol gastroprotective effects were modified via the co-administration of L-NAME and AMG.<h4>Conclusions</h4>The nano-Phytol formulation significantly enhanced the gastroprotective effect of Phytol against ethanol-induced gastric ulcers, primarily through modulation of the nitric oxide (NO) synthase pathway, suppression of inflammation, and upregulation of the growth factor TGF-β1. |
| format | Article |
| id | doaj-art-b2c50bb184bd4b8ca822a311310cf1bd |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-b2c50bb184bd4b8ca822a311310cf1bd2025-08-20T03:13:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032736810.1371/journal.pone.0327368Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation.Tamer I M RagabAbdulsalam M KassemHeba M I AbdallahSally A El AwdanNaglaa M AmmarAbd El-Nasser G El GendyBasma Emad AboulhodaSherif M AfifiTuba EsatbeyogluEnayat A OmaraAbdelsamed I Elshamy<h4>Background</h4>Innovative treatment strategies are required for stomach ulcers because of their multifactorial nature. Nanotechnology has emerged as a promising and transformative platform for the formulation and targeted delivery of therapeutic agents.<h4>Methods and findings</h4>The gastroprotective potential of both in its free form and encapsulated in calcium alginate beads was evaluated against ethanol-induced gastric ulceration in rats. Phytol-loaded nanoemulsions were incorporated into alginate beads to achieve controlled release. Alginate beads showed a pH-dependent release pattern. The release behavior showed a higher release rate at pH 6.8 than at pH 1.2. Phytol release kinetics followed the Korsmeyer-Peppas model, indicating a release mechanism governed by diffusion and polymer relaxation. Rats were pretreated with Phytol and/or nano-Phytol at 10 or 20 mg/kg doses administered one hour before ethanol exposure. Gastric ulcer was induced by administration of EtOH (1 mL/kg, p.o.) 0.5 h after NG-nitro-L-Arginine Methyl Ester (L-NAME) or Aminoguanidine (AMG) injection. Phytol treatment led to a reduction in ulcer index and severity and improved stomach gross morphology. Also, interleukin-6 (IL-6) gastric contents were reduced, whereas transforming growth factor β (TGF-β1) was elevated, and histopathological features were ameliorated. Western blot analysis revealed that nano-Phytol exerted greater inhibitory effects on caspase-3 and Nuclear Factor kappa B (NF-κB) than unformulated Phytol. Interestingly, Phytol's pharmacological effects on ulcers were enhanced by its nanoformulation in a dose-dependent way without exhibiting any toxicity symptoms. Confocal laser scanning microscopy (CLSM) confirmed significantly improved tissue penetration of nano-Phytol within the stomach layers compared to the Phytol. The Phytol or nano-Phytol gastroprotective effects were modified via the co-administration of L-NAME and AMG.<h4>Conclusions</h4>The nano-Phytol formulation significantly enhanced the gastroprotective effect of Phytol against ethanol-induced gastric ulcers, primarily through modulation of the nitric oxide (NO) synthase pathway, suppression of inflammation, and upregulation of the growth factor TGF-β1.https://doi.org/10.1371/journal.pone.0327368 |
| spellingShingle | Tamer I M Ragab Abdulsalam M Kassem Heba M I Abdallah Sally A El Awdan Naglaa M Ammar Abd El-Nasser G El Gendy Basma Emad Aboulhoda Sherif M Afifi Tuba Esatbeyoglu Enayat A Omara Abdelsamed I Elshamy Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation. PLoS ONE |
| title | Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation. |
| title_full | Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation. |
| title_fullStr | Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation. |
| title_full_unstemmed | Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation. |
| title_short | Phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol-induced gastric ulcer via nitric oxide synthase and NF-κB/IL-6/TGF-β modulation. |
| title_sort | phytol nanoemulsions encapsulated alginate hydrogel beads for the protection and management of alcohol induced gastric ulcer via nitric oxide synthase and nf κb il 6 tgf β modulation |
| url | https://doi.org/10.1371/journal.pone.0327368 |
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