High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis
Hyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes. The various functions of hyaluronan depend on its molecular size. Up to now, it has been difficult to study the role of...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2015/938013 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567012376707072 |
|---|---|
| author | Lan Do Christen P. Dahl Susanne Kerje Peter Hansell Stellan Mörner Ulla Lindqvist Anna Engström-Laurent Göran Larsson Urban Hellman |
| author_facet | Lan Do Christen P. Dahl Susanne Kerje Peter Hansell Stellan Mörner Ulla Lindqvist Anna Engström-Laurent Göran Larsson Urban Hellman |
| author_sort | Lan Do |
| collection | DOAJ |
| description | Hyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes. The various functions of hyaluronan depend on its molecular size. Up to now, it has been difficult to study the role of hyaluronan in diseases with pathological changes in the extracellular matrix where availability is low or tissue samples are small. Difficulty to obtain large enough biopsies from human diseased tissue or tissue from animal models has also restricted the study of hyaluronan. In this paper, we demonstrate that gas-phase electrophoretic molecular mobility analyzer (GEMMA) can be used to estimate the distribution of hyaluronan molecular sizes in biological samples with a limited amount of hyaluronan. The low detection level of the GEMMA method allows for estimation of hyaluronan molecular sizes from different parts of small organs. Hence, the GEMMA method opens opportunity to attain a profile over the distribution of hyaluronan molecular sizes and estimate changes caused by disease or experimental conditions that has not been possible to obtain before. |
| format | Article |
| id | doaj-art-b2be466875f7439798dbcad59c4c1076 |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-b2be466875f7439798dbcad59c4c10762025-02-03T01:02:33ZengWileyInternational Journal of Cell Biology1687-88761687-88842015-01-01201510.1155/2015/938013938013High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular AnalysisLan Do0Christen P. Dahl1Susanne Kerje2Peter Hansell3Stellan Mörner4Ulla Lindqvist5Anna Engström-Laurent6Göran Larsson7Urban Hellman8Cardiology, Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umeå University, 901 85 Umeå, SwedenDepartment of Cardiology, Oslo University Hospital, Faculty of Medicine and K.G. Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, 0327 Oslo, NorwayDepartment of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, 751 23 Uppsala, SwedenDivision of Integrative Physiology, Department of Medical Cell Biology, Uppsala University, 751 23 Uppsala, SwedenCardiology, Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umeå University, 901 85 Umeå, SwedenDepartment of Medical Sciences, Uppsala University, 751 85 Uppsala, SwedenDivision of Medicine, Department of Public Health and Clinical Medicine, Umeå University, 901 85 Umeå, SwedenDepartment of Medical Biochemistry and Biophysics-Unit of Research Education and Development Östersund, Umeå University, 831 31 Umeå, SwedenCardiology, Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umeå University, 901 85 Umeå, SwedenHyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes. The various functions of hyaluronan depend on its molecular size. Up to now, it has been difficult to study the role of hyaluronan in diseases with pathological changes in the extracellular matrix where availability is low or tissue samples are small. Difficulty to obtain large enough biopsies from human diseased tissue or tissue from animal models has also restricted the study of hyaluronan. In this paper, we demonstrate that gas-phase electrophoretic molecular mobility analyzer (GEMMA) can be used to estimate the distribution of hyaluronan molecular sizes in biological samples with a limited amount of hyaluronan. The low detection level of the GEMMA method allows for estimation of hyaluronan molecular sizes from different parts of small organs. Hence, the GEMMA method opens opportunity to attain a profile over the distribution of hyaluronan molecular sizes and estimate changes caused by disease or experimental conditions that has not been possible to obtain before.http://dx.doi.org/10.1155/2015/938013 |
| spellingShingle | Lan Do Christen P. Dahl Susanne Kerje Peter Hansell Stellan Mörner Ulla Lindqvist Anna Engström-Laurent Göran Larsson Urban Hellman High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis International Journal of Cell Biology |
| title | High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis |
| title_full | High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis |
| title_fullStr | High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis |
| title_full_unstemmed | High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis |
| title_short | High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis |
| title_sort | high sensitivity method to estimate distribution of hyaluronan molecular sizes in small biological samples using gas phase electrophoretic mobility molecular analysis |
| url | http://dx.doi.org/10.1155/2015/938013 |
| work_keys_str_mv | AT lando highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT christenpdahl highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT susannekerje highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT peterhansell highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT stellanmorner highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT ullalindqvist highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT annaengstromlaurent highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT goranlarsson highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis AT urbanhellman highsensitivitymethodtoestimatedistributionofhyaluronanmolecularsizesinsmallbiologicalsamplesusinggasphaseelectrophoreticmobilitymolecularanalysis |