Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance
Abstract Background Esophageal cancer (EC) is the sixth most common cause of cancer-related deaths globally. Genetic and environmental factors could be affected in EC's onset and development. The potential involvement of lncRNA-HOTAIR and lncRNA-MALAT1 in EC has garnered significant attention i...
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| Format: | Article |
| Language: | English |
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Springer
2025-04-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-02465-8 |
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| author | Vahid Ghorbani Saeid Ghorbian |
| author_facet | Vahid Ghorbani Saeid Ghorbian |
| author_sort | Vahid Ghorbani |
| collection | DOAJ |
| description | Abstract Background Esophageal cancer (EC) is the sixth most common cause of cancer-related deaths globally. Genetic and environmental factors could be affected in EC's onset and development. The potential involvement of lncRNA-HOTAIR and lncRNA-MALAT1 in EC has garnered significant attention in recent studies. Our investigation aimed to examine lncRNA-HOTAIR and lncRNA-MALAT1 gene expression changes in EC patients. Materials and methods Our experimental study focused on 140 patients with malignant EC, comprising 70 paraffin-embedded tumor tissues (FFPE) blocks and 70 FFPE blocks with marginal tissue samples. The relative gene expression levels of lncRNA-HOTAIR and lncRNA-MALAT1 were measured using Real-Time PCR. The data were analyzed using ANOVA and 2−△△CT tests. Results Our analysis revealed a significant increase in tumor expression compared to marginal tissues (P < 0.05). Besides, our research revealed a significant correlation between lncRNA-HOTAIR expression and hot drinks (P = 0.019), metastasis (P = 0.001), and the 5-year survival rate (P = 0.001). We found a significant correlation between lncRNA-MALAT1 expression and alcohol abuse (P = 0.039), hot drinks (P = 0.001), and metastasis (P = 0.039). Conclusion The findings indicate a potential carcinogenic effect of lncRNA-HOTAIR and lncRNA-MALAT1 gene expression alterations in EC patients. Also, studying the lncRNA genes can help us identify biomarkers, emphasizing the significance of early diagnosis and treatment. |
| format | Article |
| id | doaj-art-b2b8270842a24c5e89eb3b9c5dec0aaf |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-b2b8270842a24c5e89eb3b9c5dec0aaf2025-08-20T03:52:19ZengSpringerDiscover Oncology2730-60112025-04-011611910.1007/s12672-025-02465-8Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significanceVahid Ghorbani0Saeid Ghorbian1Department of Biology, Ah.C., Islamic Azad UniversityDepartment of Biology, Ta.C., Islamic Azad UniversityAbstract Background Esophageal cancer (EC) is the sixth most common cause of cancer-related deaths globally. Genetic and environmental factors could be affected in EC's onset and development. The potential involvement of lncRNA-HOTAIR and lncRNA-MALAT1 in EC has garnered significant attention in recent studies. Our investigation aimed to examine lncRNA-HOTAIR and lncRNA-MALAT1 gene expression changes in EC patients. Materials and methods Our experimental study focused on 140 patients with malignant EC, comprising 70 paraffin-embedded tumor tissues (FFPE) blocks and 70 FFPE blocks with marginal tissue samples. The relative gene expression levels of lncRNA-HOTAIR and lncRNA-MALAT1 were measured using Real-Time PCR. The data were analyzed using ANOVA and 2−△△CT tests. Results Our analysis revealed a significant increase in tumor expression compared to marginal tissues (P < 0.05). Besides, our research revealed a significant correlation between lncRNA-HOTAIR expression and hot drinks (P = 0.019), metastasis (P = 0.001), and the 5-year survival rate (P = 0.001). We found a significant correlation between lncRNA-MALAT1 expression and alcohol abuse (P = 0.039), hot drinks (P = 0.001), and metastasis (P = 0.039). Conclusion The findings indicate a potential carcinogenic effect of lncRNA-HOTAIR and lncRNA-MALAT1 gene expression alterations in EC patients. Also, studying the lncRNA genes can help us identify biomarkers, emphasizing the significance of early diagnosis and treatment.https://doi.org/10.1007/s12672-025-02465-8LncRNAHOTAIRMALAT1Esophageal cancer |
| spellingShingle | Vahid Ghorbani Saeid Ghorbian Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance Discover Oncology LncRNA HOTAIR MALAT1 Esophageal cancer |
| title | Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance |
| title_full | Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance |
| title_fullStr | Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance |
| title_full_unstemmed | Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance |
| title_short | Gene expression profiling of lncRNA-HOTAIR and lncRNA-MALAT1 in esophageal cancer: uncovering links to lifestyle factors and diagnostic significance |
| title_sort | gene expression profiling of lncrna hotair and lncrna malat1 in esophageal cancer uncovering links to lifestyle factors and diagnostic significance |
| topic | LncRNA HOTAIR MALAT1 Esophageal cancer |
| url | https://doi.org/10.1007/s12672-025-02465-8 |
| work_keys_str_mv | AT vahidghorbani geneexpressionprofilingoflncrnahotairandlncrnamalat1inesophagealcanceruncoveringlinkstolifestylefactorsanddiagnosticsignificance AT saeidghorbian geneexpressionprofilingoflncrnahotairandlncrnamalat1inesophagealcanceruncoveringlinkstolifestylefactorsanddiagnosticsignificance |