Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease
Chronic kidney disease (CKD) as one of the most common complications of type 2 diabetes mellitus (T2DM) significantly increases the risk of cardiovascular disease and mortality. Mitochondrial dysfunction, in particular a reduction in mitochondrial DNA copy number (mtDNA-CN), plays an important role...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2025-04-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2025/05/Saienko_97_2.pdf |
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| author | Y. A. Saienko D. S. Krasnenkov K. K. Midlovets V. V. Korcheva Y. E. Rebrova D. D. Yepishyna B. M. Mankovsky |
| author_facet | Y. A. Saienko D. S. Krasnenkov K. K. Midlovets V. V. Korcheva Y. E. Rebrova D. D. Yepishyna B. M. Mankovsky |
| author_sort | Y. A. Saienko |
| collection | DOAJ |
| description | Chronic kidney disease (CKD) as one of the most common complications of type 2 diabetes mellitus (T2DM) significantly increases the risk of cardiovascular disease and mortality. Mitochondrial dysfunction, in particular a reduction in mitochondrial DNA copy number (mtDNA-CN), plays an important role in the development of diabetic complications, including nephropathy. The aim of this study was to determine the mtDNA-CN in peripheral blood leukocytes of patients with T2DM depending on the presence of CKD. A total of 109 individuals were examined, including 20 healthy controls and 89 patients with T2DM divided into groups based on the presence or absence of CKD. The mtDNA-CN in leukocytes was determined using quantitative real-time PCR. Biochemical markers of T2DM and CKD were evaluated, non-parametric tests and correlation analysis were performed. No statistically significant differences in mtDNA-CN level were observed between patients with T2DM and CKD, patients with T2DM without CKD, and the control group (P > 0.05). No associations between mtDNA-CN and kidney function parameters were identified. The absence of mtDNA-CN alterations is assumed to contribute to the relatively satisfactory glycemic control in diabetic groups. |
| format | Article |
| id | doaj-art-b2af18cb4e9344719a8c4f3adaf63274 |
| institution | DOAJ |
| issn | 2409-4943 2413-5003 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj-art-b2af18cb4e9344719a8c4f3adaf632742025-08-20T02:41:36ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032025-04-01972414710.15407/ubj97.02.041Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney diseaseY. A. Saienko 0D. S. Krasnenkov 1K. K. Midlovets 2V. V. Korcheva3https://orcid.org/0009-0008-0894-7836Y. E. Rebrova 4D. D. Yepishyna 5B. M. Mankovsky6https://orcid.org/0000-0001-8289-3604SI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine; SI “Center for Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine”, Kyiv, Ukraine SI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, UkraineSI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, UkraineSI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, UkraineSI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine; P.L. Shupyk National University of Health Care of Ukraine, Kyiv, UkraineSI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, UkraineSI “D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine”, Kyiv, UkraineChronic kidney disease (CKD) as one of the most common complications of type 2 diabetes mellitus (T2DM) significantly increases the risk of cardiovascular disease and mortality. Mitochondrial dysfunction, in particular a reduction in mitochondrial DNA copy number (mtDNA-CN), plays an important role in the development of diabetic complications, including nephropathy. The aim of this study was to determine the mtDNA-CN in peripheral blood leukocytes of patients with T2DM depending on the presence of CKD. A total of 109 individuals were examined, including 20 healthy controls and 89 patients with T2DM divided into groups based on the presence or absence of CKD. The mtDNA-CN in leukocytes was determined using quantitative real-time PCR. Biochemical markers of T2DM and CKD were evaluated, non-parametric tests and correlation analysis were performed. No statistically significant differences in mtDNA-CN level were observed between patients with T2DM and CKD, patients with T2DM without CKD, and the control group (P > 0.05). No associations between mtDNA-CN and kidney function parameters were identified. The absence of mtDNA-CN alterations is assumed to contribute to the relatively satisfactory glycemic control in diabetic groups.http://ukrbiochemjournal.org/wp-content/uploads/2025/05/Saienko_97_2.pdfchronic kidney diseaseleukocytesmitochondrial dnamtdna copy numbertype 2 diabetes mellitus |
| spellingShingle | Y. A. Saienko D. S. Krasnenkov K. K. Midlovets V. V. Korcheva Y. E. Rebrova D. D. Yepishyna B. M. Mankovsky Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease The Ukrainian Biochemical Journal chronic kidney disease leukocytes mitochondrial dna mtdna copy number type 2 diabetes mellitus |
| title | Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease |
| title_full | Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease |
| title_fullStr | Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease |
| title_full_unstemmed | Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease |
| title_short | Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease |
| title_sort | mitochondrial dna copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease |
| topic | chronic kidney disease leukocytes mitochondrial dna mtdna copy number type 2 diabetes mellitus |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2025/05/Saienko_97_2.pdf |
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