Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India

Abstract Whitefly, Bemisia tabaci is a globally recognized invasive cryptic pest species complex and a primary vector for 90% of begomoviruses. Understanding the species composition and diversity within the B. tabaci cryptic species complex is essential for developing effective pest management strat...

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Main Authors: Kurubarahalli Bhoothanna Ramesh, Chowdanayak Mahendra, M. N. Rudra Gouda, Rajna Salim, Sabtharshi Subramanian
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-96483-9
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author Kurubarahalli Bhoothanna Ramesh
Chowdanayak Mahendra
M. N. Rudra Gouda
Rajna Salim
Sabtharshi Subramanian
author_facet Kurubarahalli Bhoothanna Ramesh
Chowdanayak Mahendra
M. N. Rudra Gouda
Rajna Salim
Sabtharshi Subramanian
author_sort Kurubarahalli Bhoothanna Ramesh
collection DOAJ
description Abstract Whitefly, Bemisia tabaci is a globally recognized invasive cryptic pest species complex and a primary vector for 90% of begomoviruses. Understanding the species composition and diversity within the B. tabaci cryptic species complex is essential for developing effective pest management strategies. The Asia II 1 genetic group of B. tabaci is notably widespread in India and across Asia, demonstrating significant genetic diversity. Our study investigates the haplotype diversity of Asia II 1 using the mtCOI barcoding gene, analyzing 676 sequences from various Asian countries and 190 sequences from India. We identified 241 distinct haplotypes in Asia II 1 across Asia, with the highest haplotype diversity in China (Hd: 1.000) and the lowest in Vietnam (Hd: 0.667). Nucleotide diversity peaked in Pakistan (pi: 0.0145) and was lowest in Vietnam (pi: 0.0010). In India, we identified 77 haplotypes with a diversity of 0.926 and nucleotide diversity of 0.0076. When grouped by hostplant families, 79 haplotypes were recorded, with the highest diversity in Cucurbitaceae and the lowest in Solanaceae. Our findings suggest that hostplants and geographical location significantly influence genetic group development, offering novel insights into Asia II 1’s genetic structure and evolution. This marks the first comprehensive study of Asia II 1 genetic diversity in Asia and India.
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spelling doaj-art-b2a9246750694ce89cb5f6fffc8fe06d2025-08-20T01:52:55ZengNature PortfolioScientific Reports2045-23222025-04-0115111310.1038/s41598-025-96483-9Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and IndiaKurubarahalli Bhoothanna Ramesh0Chowdanayak Mahendra1M. N. Rudra Gouda2Rajna Salim3Sabtharshi Subramanian4Division of Entomology, ICAR-Indian Agricultural Research InstituteDivision of Molecular Biology and Biotechnology, ICAR- Indian Agricultural Research InstituteDivision of Entomology, ICAR-Indian Agricultural Research InstituteDivision of Entomology, ICAR-Indian Agricultural Research InstituteDivision of Entomology, ICAR-Indian Agricultural Research InstituteAbstract Whitefly, Bemisia tabaci is a globally recognized invasive cryptic pest species complex and a primary vector for 90% of begomoviruses. Understanding the species composition and diversity within the B. tabaci cryptic species complex is essential for developing effective pest management strategies. The Asia II 1 genetic group of B. tabaci is notably widespread in India and across Asia, demonstrating significant genetic diversity. Our study investigates the haplotype diversity of Asia II 1 using the mtCOI barcoding gene, analyzing 676 sequences from various Asian countries and 190 sequences from India. We identified 241 distinct haplotypes in Asia II 1 across Asia, with the highest haplotype diversity in China (Hd: 1.000) and the lowest in Vietnam (Hd: 0.667). Nucleotide diversity peaked in Pakistan (pi: 0.0145) and was lowest in Vietnam (pi: 0.0010). In India, we identified 77 haplotypes with a diversity of 0.926 and nucleotide diversity of 0.0076. When grouped by hostplant families, 79 haplotypes were recorded, with the highest diversity in Cucurbitaceae and the lowest in Solanaceae. Our findings suggest that hostplants and geographical location significantly influence genetic group development, offering novel insights into Asia II 1’s genetic structure and evolution. This marks the first comprehensive study of Asia II 1 genetic diversity in Asia and India.https://doi.org/10.1038/s41598-025-96483-9Bemisia tabaciAsia II 1Haplotype diversityAsia
spellingShingle Kurubarahalli Bhoothanna Ramesh
Chowdanayak Mahendra
M. N. Rudra Gouda
Rajna Salim
Sabtharshi Subramanian
Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India
Scientific Reports
Bemisia tabaci
Asia II 1
Haplotype diversity
Asia
title Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India
title_full Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India
title_fullStr Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India
title_full_unstemmed Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India
title_short Genetic structure and haplotype analysis of predominant genetic group of Bemisia tabaci Asia II 1 from Asia and India
title_sort genetic structure and haplotype analysis of predominant genetic group of bemisia tabaci asia ii 1 from asia and india
topic Bemisia tabaci
Asia II 1
Haplotype diversity
Asia
url https://doi.org/10.1038/s41598-025-96483-9
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