High salicylic acid concentration alters the electron flow associated with photosystem II in barley
In this study, the effects of exogenously applied salicylic acid (0.5 and 5 mM SA) on the rates of photosystem II (PSII) activity was analysed in 4-week-old barley (Hordeum vulgare‘Bahman’ ) seedlings using chlorophyll (Chl) a fluorescence transient (OJIP) measurements. No evident changes in Chl and...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2017-09-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/12731 |
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| author | Ghader Habibi Atousa Vaziri |
| author_facet | Ghader Habibi Atousa Vaziri |
| author_sort | Ghader Habibi |
| collection | DOAJ |
| description | In this study, the effects of exogenously applied salicylic acid (0.5 and 5 mM SA) on the rates of photosystem II (PSII) activity was analysed in 4-week-old barley (Hordeum vulgare‘Bahman’ ) seedlings using chlorophyll (Chl) a fluorescence transient (OJIP) measurements. No evident changes in Chl and carotenoid contents as well as chlorophyll fluorescence transient curves were observed in either of the studied concentrations after 24 h of SA application. After 5 d, low SA concentration (0.5 mM) increased PSII activity, Chl b and carotenoid contents in barley seedlings. In contrary, 5 days after 5 mM SA treatment, the maximal quantum efficiency of PSII (Fv/Fm) and the Performance Index (PIABS), as an indicator of PSII structure and functioning, were significantly decreased. This lower Fv/Fm and PIABS coupled with lower levels of Chl b and carotenoids, and lower values of photosynthetic electron transport chain components including the electron transport flux (φEo) and the inferred oxygen evolving complex activity (Fv/Fo). By monitoring the chlorophyll a fluorescence rise kinetics, from the initial “O” level to the “P” (the peak) level, a dramatic increase in “OJ” phase was detected, which coincides with an increased photo-reduction of QA as a result of blockage of electron flow. This study provided the evidence that the high concentration of SA induced damage to different sites of the PSII. |
| format | Article |
| id | doaj-art-b2a5cd1dda2045f9994c309d200fbecd |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-b2a5cd1dda2045f9994c309d200fbecd2025-08-20T03:02:26ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412017-09-01109239340210.14720/aas.2017.109.2.2219123High salicylic acid concentration alters the electron flow associated with photosystem II in barleyGhader Habibi0Atousa Vaziri1Department of Biology, Payame Noor University (PNU), IRANDepartment of Biology, Payame Noor University (PNU), IRANIn this study, the effects of exogenously applied salicylic acid (0.5 and 5 mM SA) on the rates of photosystem II (PSII) activity was analysed in 4-week-old barley (Hordeum vulgare‘Bahman’ ) seedlings using chlorophyll (Chl) a fluorescence transient (OJIP) measurements. No evident changes in Chl and carotenoid contents as well as chlorophyll fluorescence transient curves were observed in either of the studied concentrations after 24 h of SA application. After 5 d, low SA concentration (0.5 mM) increased PSII activity, Chl b and carotenoid contents in barley seedlings. In contrary, 5 days after 5 mM SA treatment, the maximal quantum efficiency of PSII (Fv/Fm) and the Performance Index (PIABS), as an indicator of PSII structure and functioning, were significantly decreased. This lower Fv/Fm and PIABS coupled with lower levels of Chl b and carotenoids, and lower values of photosynthetic electron transport chain components including the electron transport flux (φEo) and the inferred oxygen evolving complex activity (Fv/Fo). By monitoring the chlorophyll a fluorescence rise kinetics, from the initial “O” level to the “P” (the peak) level, a dramatic increase in “OJ” phase was detected, which coincides with an increased photo-reduction of QA as a result of blockage of electron flow. This study provided the evidence that the high concentration of SA induced damage to different sites of the PSII.https://journals.uni-lj.si/aas/article/view/12731photosynthetic pigmentsphotosynthetic electron flowhordeum vulgare ‘bahman’ojip transient flourescencesalicylic acid |
| spellingShingle | Ghader Habibi Atousa Vaziri High salicylic acid concentration alters the electron flow associated with photosystem II in barley Acta Agriculturae Slovenica photosynthetic pigments photosynthetic electron flow hordeum vulgare ‘bahman’ ojip transient flourescence salicylic acid |
| title | High salicylic acid concentration alters the electron flow associated with photosystem II in barley |
| title_full | High salicylic acid concentration alters the electron flow associated with photosystem II in barley |
| title_fullStr | High salicylic acid concentration alters the electron flow associated with photosystem II in barley |
| title_full_unstemmed | High salicylic acid concentration alters the electron flow associated with photosystem II in barley |
| title_short | High salicylic acid concentration alters the electron flow associated with photosystem II in barley |
| title_sort | high salicylic acid concentration alters the electron flow associated with photosystem ii in barley |
| topic | photosynthetic pigments photosynthetic electron flow hordeum vulgare ‘bahman’ ojip transient flourescence salicylic acid |
| url | https://journals.uni-lj.si/aas/article/view/12731 |
| work_keys_str_mv | AT ghaderhabibi highsalicylicacidconcentrationalterstheelectronflowassociatedwithphotosystemiiinbarley AT atousavaziri highsalicylicacidconcentrationalterstheelectronflowassociatedwithphotosystemiiinbarley |