Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations
Three simple, sensitive, accurate, and rapid visible spectrophotometric methods (A, B, and C) have been developed for the estimation of nitrazepam in both pure and in pharmaceutical preparations. They are based on the diazotization of reduced nitrazepam with nitrous acid followed by coupling with ac...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/671689 |
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| author | Hemavathi Nagaraju Deepakumari Hosakere Doddarevanna Revanasiddappa |
| author_facet | Hemavathi Nagaraju Deepakumari Hosakere Doddarevanna Revanasiddappa |
| author_sort | Hemavathi Nagaraju Deepakumari |
| collection | DOAJ |
| description | Three simple, sensitive, accurate, and rapid visible spectrophotometric methods (A, B, and C) have been developed for the estimation of nitrazepam in both pure and in pharmaceutical preparations. They are based on the diazotization of reduced nitrazepam with nitrous acid followed by coupling with acetyl acetone (method A), diphenylamine (method B), and citrazinic acid (method C) to form colored azo-dyes, exhibiting absorption maxima () at 400, 550, and 460 nm, for methods A, B, and C, respectively. The produced colored azo-dyes are stable for more than 2 h. Beer's law was obeyed in the concentration range of 0.5–20, 0.3–14 and 0.5–12 μg/mL for methods A, B, and C, respectively and the corresponding molar absorptivity values are , , and L mol−1cm−1. All variables have been optimized and the results were statistically compared with those of a literature method by employing the Student's t-test and F-test. No interference was observed from common adjuvants normally added to the tablets. The results obtained in the proposed methods are in good agreement with labeled amounts, when marketed pharmaceutical preparations are analyzed, which could be applied in the routine quality control analysis laboratory. |
| format | Article |
| id | doaj-art-b2a12f649e5f4815acfd982acaf737c1 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-b2a12f649e5f4815acfd982acaf737c12025-02-03T01:31:00ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/671689671689Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical PreparationsHemavathi Nagaraju Deepakumari0Hosakere Doddarevanna Revanasiddappa1Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, IndiaDepartment of Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, IndiaThree simple, sensitive, accurate, and rapid visible spectrophotometric methods (A, B, and C) have been developed for the estimation of nitrazepam in both pure and in pharmaceutical preparations. They are based on the diazotization of reduced nitrazepam with nitrous acid followed by coupling with acetyl acetone (method A), diphenylamine (method B), and citrazinic acid (method C) to form colored azo-dyes, exhibiting absorption maxima () at 400, 550, and 460 nm, for methods A, B, and C, respectively. The produced colored azo-dyes are stable for more than 2 h. Beer's law was obeyed in the concentration range of 0.5–20, 0.3–14 and 0.5–12 μg/mL for methods A, B, and C, respectively and the corresponding molar absorptivity values are , , and L mol−1cm−1. All variables have been optimized and the results were statistically compared with those of a literature method by employing the Student's t-test and F-test. No interference was observed from common adjuvants normally added to the tablets. The results obtained in the proposed methods are in good agreement with labeled amounts, when marketed pharmaceutical preparations are analyzed, which could be applied in the routine quality control analysis laboratory.http://dx.doi.org/10.1155/2013/671689 |
| spellingShingle | Hemavathi Nagaraju Deepakumari Hosakere Doddarevanna Revanasiddappa Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations Journal of Spectroscopy |
| title | Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations |
| title_full | Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations |
| title_fullStr | Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations |
| title_full_unstemmed | Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations |
| title_short | Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations |
| title_sort | spectrophotometric estimation of nitrazepam in pure and in pharmaceutical preparations |
| url | http://dx.doi.org/10.1155/2013/671689 |
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