Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate
In this study, oligosaccharide from Caulerpa racemosa var. peltate (CRVO-G) was prepared by sequential enzymatic hydrolysis with lactase, membrane filtration, dialysis, and freeze-drying, and the relative molecular mass and monosaccharide composition of CRVO-G were measured. Meanwhile, the immunoreg...
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China Food Publishing Company
2024-09-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-17-006.pdf |
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| author | ZENG Jinzi, LIN Qianmin, XU Jingyi, XU Baojun, Io Nam Wong, HUANG Riming |
| author_facet | ZENG Jinzi, LIN Qianmin, XU Jingyi, XU Baojun, Io Nam Wong, HUANG Riming |
| author_sort | ZENG Jinzi, LIN Qianmin, XU Jingyi, XU Baojun, Io Nam Wong, HUANG Riming |
| collection | DOAJ |
| description | In this study, oligosaccharide from Caulerpa racemosa var. peltate (CRVO-G) was prepared by sequential enzymatic hydrolysis with lactase, membrane filtration, dialysis, and freeze-drying, and the relative molecular mass and monosaccharide composition of CRVO-G were measured. Meanwhile, the immunoregulatory activity of CRVO-G was evaluated and its mechanism of action was studied. The results showed that CRVO-G was a mixture of two tetrasaccharides and one hexasaccharide with relative molecular masses of 617.115, 621.081, and 995.368, and was mainly composed of galactose (51.0%), mannose (21.3%), glucose (11.5%), xylose (6.5%), rhamnose (2.3%), glucuronic acid (2.0%), glucosamine hydrochloride (1.8%), fucose (0.8%), glucosamine hydrochloride (0.7%), galacturonic acid (0.7%), mannuronic acid (0.7%), and arabinose (0.6%). In addition, CRVO-G had good immunomodulatory activity, as manifested by increased release of nitric oxide and cytokines (interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α). The results of metabolomics analysis and Western blot showed that CRVO-G could promote the synthesis of thromboxane A2 (TXA2) and 15-keto prostaglandin F2α (15-keto-PGF2α) in the arachidonic acid pathway of macrophages, and increase the expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and the phosphorylation of nuclear factor κB (NF-κB). In summary, CRVO-G exerts immunomodulatory effects by the NF-κB/iNOS/COX-2 pathway in RAW264.7 macrophages. |
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| id | doaj-art-b29d030398ee43d7973957dd0bb55eb3 |
| institution | OA Journals |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-b29d030398ee43d7973957dd0bb55eb32025-08-20T02:17:52ZengChina Food Publishing CompanyShipin Kexue1002-66302024-09-014517425110.7506/spkx1002-6630-20231027-238Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltateZENG Jinzi, LIN Qianmin, XU Jingyi, XU Baojun, Io Nam Wong, HUANG Riming0(1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Department of Life Science, BNU-HKBU United International College, Zhuhai 519087, China; 3. Faculty of Medicine, Macau University of Science and Technology, Macau 999078, China)In this study, oligosaccharide from Caulerpa racemosa var. peltate (CRVO-G) was prepared by sequential enzymatic hydrolysis with lactase, membrane filtration, dialysis, and freeze-drying, and the relative molecular mass and monosaccharide composition of CRVO-G were measured. Meanwhile, the immunoregulatory activity of CRVO-G was evaluated and its mechanism of action was studied. The results showed that CRVO-G was a mixture of two tetrasaccharides and one hexasaccharide with relative molecular masses of 617.115, 621.081, and 995.368, and was mainly composed of galactose (51.0%), mannose (21.3%), glucose (11.5%), xylose (6.5%), rhamnose (2.3%), glucuronic acid (2.0%), glucosamine hydrochloride (1.8%), fucose (0.8%), glucosamine hydrochloride (0.7%), galacturonic acid (0.7%), mannuronic acid (0.7%), and arabinose (0.6%). In addition, CRVO-G had good immunomodulatory activity, as manifested by increased release of nitric oxide and cytokines (interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α). The results of metabolomics analysis and Western blot showed that CRVO-G could promote the synthesis of thromboxane A2 (TXA2) and 15-keto prostaglandin F2α (15-keto-PGF2α) in the arachidonic acid pathway of macrophages, and increase the expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and the phosphorylation of nuclear factor κB (NF-κB). In summary, CRVO-G exerts immunomodulatory effects by the NF-κB/iNOS/COX-2 pathway in RAW264.7 macrophages.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-17-006.pdfcaulerpa racemosa var. peltate; oligosaccharide; enzymatic hydrolysis; immunomodulatory; molecular mechanism |
| spellingShingle | ZENG Jinzi, LIN Qianmin, XU Jingyi, XU Baojun, Io Nam Wong, HUANG Riming Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate Shipin Kexue caulerpa racemosa var. peltate; oligosaccharide; enzymatic hydrolysis; immunomodulatory; molecular mechanism |
| title | Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate |
| title_full | Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate |
| title_fullStr | Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate |
| title_full_unstemmed | Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate |
| title_short | Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var. peltate |
| title_sort | enzymatic preparation and immunomodulatory mechanism of oligosaccharide from caulerpa racemosa var peltate |
| topic | caulerpa racemosa var. peltate; oligosaccharide; enzymatic hydrolysis; immunomodulatory; molecular mechanism |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-17-006.pdf |
| work_keys_str_mv | AT zengjinzilinqianminxujingyixubaojunionamwonghuangriming enzymaticpreparationandimmunomodulatorymechanismofoligosaccharidefromcaulerparacemosavarpeltate |