Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening
The purpose of the current study was to determine the effect of leaf shading, fruit shading, and a combination of both, on the accumulation of ascorbic acid (AsA) and the expression levels of AsA biosynthetic genes at the immature green, mature green, breaker, and red ripe stages of Ailsa craig toma...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2022-01-01
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| Series: | Vegetable Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/VR-2022-0001 |
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| author | Safir Ahmad Tamim Fangman Li Ying Wang Lele Shang Xingyu Zhang Jinbao Tao Yaru Wang Wenxian Gai Haiqiang Dong John Kojo Ahiakpa Muhammad Ali Mumtaz Yuyang Zhang |
| author_facet | Safir Ahmad Tamim Fangman Li Ying Wang Lele Shang Xingyu Zhang Jinbao Tao Yaru Wang Wenxian Gai Haiqiang Dong John Kojo Ahiakpa Muhammad Ali Mumtaz Yuyang Zhang |
| author_sort | Safir Ahmad Tamim |
| collection | DOAJ |
| description | The purpose of the current study was to determine the effect of leaf shading, fruit shading, and a combination of both, on the accumulation of ascorbic acid (AsA) and the expression levels of AsA biosynthetic genes at the immature green, mature green, breaker, and red ripe stages of Ailsa craig tomato during fruit development. Shading (72% reducing of light intensity) imposed on the leaves significantly reduced AsA content and AsA biosynthetic gene expression in the fruits. Leaf shading, fruit shading, and a combination of both significantly decreased the amount of total AsA and reduced AsA to a range of 18.5%−31.5% at mature green, breaker, and red ripe stages of tomato fruits, with no significant change at the immature green stage of fruits. Moreover, reducing the light intensity in tomato leaves, fruits or both resulted in reduced expression of most AsA biosynthetic genes in the fruits, except for PMM, cAPX, tAPX, and APX7 genes under leaf shading, GPI, PMI, PMM, GP1, GP2, cAPX, and tAPX genes under fruit shading, and PMM, cAPX, APX1, and APX7 genes under both shading. The expression level of GMP, GP1, and GalDH showed an up-regulation at the red ripe stage in fruits with leaf shading, and also an up-regulation at the immature green and red ripe stages with both shading. Furthermore, positive correlations between expression of AsA biosynthetic genes and AsA accumulation were recorded under leaf shading, fruit shading, and both types of shading, while a negative correlation was recorded under normal conditions without shading. |
| format | Article |
| id | doaj-art-b29c16afd93a4b819935118d439a22aa |
| institution | DOAJ |
| issn | 2769-0520 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Vegetable Research |
| spelling | doaj-art-b29c16afd93a4b819935118d439a22aa2025-08-20T03:18:33ZengMaximum Academic PressVegetable Research2769-05202022-01-01211810.48130/VR-2022-0001VR-2022-0001Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripeningSafir Ahmad Tamim0Fangman Li1Ying Wang2Lele Shang3Xingyu Zhang4Jinbao Tao5Yaru Wang6Wenxian Gai7Haiqiang Dong8John Kojo Ahiakpa9Muhammad Ali Mumtaz10Yuyang Zhang11Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaThe purpose of the current study was to determine the effect of leaf shading, fruit shading, and a combination of both, on the accumulation of ascorbic acid (AsA) and the expression levels of AsA biosynthetic genes at the immature green, mature green, breaker, and red ripe stages of Ailsa craig tomato during fruit development. Shading (72% reducing of light intensity) imposed on the leaves significantly reduced AsA content and AsA biosynthetic gene expression in the fruits. Leaf shading, fruit shading, and a combination of both significantly decreased the amount of total AsA and reduced AsA to a range of 18.5%−31.5% at mature green, breaker, and red ripe stages of tomato fruits, with no significant change at the immature green stage of fruits. Moreover, reducing the light intensity in tomato leaves, fruits or both resulted in reduced expression of most AsA biosynthetic genes in the fruits, except for PMM, cAPX, tAPX, and APX7 genes under leaf shading, GPI, PMI, PMM, GP1, GP2, cAPX, and tAPX genes under fruit shading, and PMM, cAPX, APX1, and APX7 genes under both shading. The expression level of GMP, GP1, and GalDH showed an up-regulation at the red ripe stage in fruits with leaf shading, and also an up-regulation at the immature green and red ripe stages with both shading. Furthermore, positive correlations between expression of AsA biosynthetic genes and AsA accumulation were recorded under leaf shading, fruit shading, and both types of shading, while a negative correlation was recorded under normal conditions without shading.https://www.maxapress.com/article/doi/10.48130/VR-2022-0001solanum lycopersicumlight intensityascorbic acidbiosynthesisgene expression |
| spellingShingle | Safir Ahmad Tamim Fangman Li Ying Wang Lele Shang Xingyu Zhang Jinbao Tao Yaru Wang Wenxian Gai Haiqiang Dong John Kojo Ahiakpa Muhammad Ali Mumtaz Yuyang Zhang Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening Vegetable Research solanum lycopersicum light intensity ascorbic acid biosynthesis gene expression |
| title | Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening |
| title_full | Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening |
| title_fullStr | Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening |
| title_full_unstemmed | Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening |
| title_short | Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening |
| title_sort | effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening |
| topic | solanum lycopersicum light intensity ascorbic acid biosynthesis gene expression |
| url | https://www.maxapress.com/article/doi/10.48130/VR-2022-0001 |
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