In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet.
Non-thermal atmospheric-pressure plasma (NTAPP) has been widely studied for clinical applications, e.g., disinfection, wound healing, cancer therapy, hemostasis, and bone regeneration. It is being revealed that the physical and chemical actions of plasma have enabled these clinical applications. Bas...
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0301216&type=printable |
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| author | Katusmasa Nakazawa Hiromitsu Toyoda Tomoya Manaka Kumi Orita Yoshihiro Hirakawa Kosuke Saito Ryosuke Iio Akiyoshi Shimatani Yoshitaka Ban Hana Yao Ryosuke Otsuki Yamato Torii Jun-Seok Oh Tatsuru Shirafuji Hiroaki Nakamura |
| author_facet | Katusmasa Nakazawa Hiromitsu Toyoda Tomoya Manaka Kumi Orita Yoshihiro Hirakawa Kosuke Saito Ryosuke Iio Akiyoshi Shimatani Yoshitaka Ban Hana Yao Ryosuke Otsuki Yamato Torii Jun-Seok Oh Tatsuru Shirafuji Hiroaki Nakamura |
| author_sort | Katusmasa Nakazawa |
| collection | DOAJ |
| description | Non-thermal atmospheric-pressure plasma (NTAPP) has been widely studied for clinical applications, e.g., disinfection, wound healing, cancer therapy, hemostasis, and bone regeneration. It is being revealed that the physical and chemical actions of plasma have enabled these clinical applications. Based on our previous report regarding plasma-stimulated bone regeneration, this study focused on Achilles tendon repair by NTAPP. This is the first study to reveal that exposure to NTAPP can accelerate Achilles tendon repair using a well-established Achilles tendon injury rat model. Histological evaluation using the Stoll's and histological scores showed a significant improvement at 2 and 4 weeks, with type I collagen content being substantial at the early time point of 2 weeks post-surgery. Notably, the replacement of type III collagen with type I collagen occurred more frequently in the plasma-treated groups at the early stage of repair. Tensile strength test results showed that the maximum breaking strength in the plasma-treated group at two weeks was significantly higher than that in the untreated group. Overall, our results indicate that a single event of NTAPP treatment during the surgery can contribute to an early recovery of an injured tendon. |
| format | Article |
| id | doaj-art-b292e591ded941a3874d2a6739b96d52 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b292e591ded941a3874d2a6739b96d522025-08-20T01:56:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01195e030121610.1371/journal.pone.0301216In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet.Katusmasa NakazawaHiromitsu ToyodaTomoya ManakaKumi OritaYoshihiro HirakawaKosuke SaitoRyosuke IioAkiyoshi ShimataniYoshitaka BanHana YaoRyosuke OtsukiYamato ToriiJun-Seok OhTatsuru ShirafujiHiroaki NakamuraNon-thermal atmospheric-pressure plasma (NTAPP) has been widely studied for clinical applications, e.g., disinfection, wound healing, cancer therapy, hemostasis, and bone regeneration. It is being revealed that the physical and chemical actions of plasma have enabled these clinical applications. Based on our previous report regarding plasma-stimulated bone regeneration, this study focused on Achilles tendon repair by NTAPP. This is the first study to reveal that exposure to NTAPP can accelerate Achilles tendon repair using a well-established Achilles tendon injury rat model. Histological evaluation using the Stoll's and histological scores showed a significant improvement at 2 and 4 weeks, with type I collagen content being substantial at the early time point of 2 weeks post-surgery. Notably, the replacement of type III collagen with type I collagen occurred more frequently in the plasma-treated groups at the early stage of repair. Tensile strength test results showed that the maximum breaking strength in the plasma-treated group at two weeks was significantly higher than that in the untreated group. Overall, our results indicate that a single event of NTAPP treatment during the surgery can contribute to an early recovery of an injured tendon.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0301216&type=printable |
| spellingShingle | Katusmasa Nakazawa Hiromitsu Toyoda Tomoya Manaka Kumi Orita Yoshihiro Hirakawa Kosuke Saito Ryosuke Iio Akiyoshi Shimatani Yoshitaka Ban Hana Yao Ryosuke Otsuki Yamato Torii Jun-Seok Oh Tatsuru Shirafuji Hiroaki Nakamura In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet. PLoS ONE |
| title | In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet. |
| title_full | In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet. |
| title_fullStr | In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet. |
| title_full_unstemmed | In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet. |
| title_short | In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet. |
| title_sort | in vivo study on the repair of rat achilles tendon injury treated with non thermal atmospheric pressure helium microplasma jet |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0301216&type=printable |
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