PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis
Objectives: The diagnostic role and correlation between phosphatase and tensin homologue and alpha-smooth muscle actin in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis was analysed by this case control study. The mechanism by which phosphatase and...
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Lithuanian University of Health Sciences, Faculty of Odontology
2021-03-01
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| Series: | eJournal of Oral Maxillofacial Research |
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| Online Access: | https://www.ejomr.org/JOMR/archives/2021/1/e3/v12n1e3ht.htm |
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| author | Roshni Monteiro Kaveri Hallikeri Archana Sudhakaran |
| author_facet | Roshni Monteiro Kaveri Hallikeri Archana Sudhakaran |
| author_sort | Roshni Monteiro |
| collection | DOAJ |
| description | Objectives: The diagnostic role and correlation between phosphatase and tensin homologue and alpha-smooth muscle actin in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis was analysed by this case control study. The mechanism by which phosphatase and tensin homologue controls myofibroblast expression was also evaluated.
Material and Methods: Overall, 10 normal mucosa, 30 oral submucous fibrosis (OSF) and 30 oral squamous cell carcinoma (OSCC) with OSF were stained immunohistochemically with phosphatase and tensin homologue (PTEN) and alpha-smooth muscle actin (α-SMA). Percentage positivity, pattern of expression was statistically compared using Pearson’s Chi-square and Fischer exact tests. The correlation between markers was analysed using Spearman correlation.
Results: OSF and OSCC affected males predominantly with majority below 40 years and above 40 years of age respectively. Percentage of PTEN positive cells was statistically significant with gender (P = 0.024) and α-SMA distribution of pattern showed a significant correlation with habits (P = 0.018). A significant decrease in nuclear PTEN positivity (P < 0.001) and a gradual increase in α-SMA cytoplasmic expression was noted from NM to OSF and OSCC. A statistically significant weak inverse correlation existed between PTEN and α-SMA.
Conclusions: A reduced phosphatase and tensin homologue expression in oral submucous fibrosis makes it more prone for malignant transformation. An increase in stromal desmoplasia modifies differentiation, invasive and proliferative capacity of tumour cells. As phosphatase and tensin homologue functions through P-Akt pathway, P-Akt with phosphatase and tensin homologue could be a therapeutic target. |
| format | Article |
| id | doaj-art-b28c84641ba74b1682afbcda583e937b |
| institution | DOAJ |
| issn | 2029-283X |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Lithuanian University of Health Sciences, Faculty of Odontology |
| record_format | Article |
| series | eJournal of Oral Maxillofacial Research |
| spelling | doaj-art-b28c84641ba74b1682afbcda583e937b2025-08-20T03:20:13ZengLithuanian University of Health Sciences, Faculty of OdontologyeJournal of Oral Maxillofacial Research2029-283X2021-03-01121e310.5037/jomr.2021.12103PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous FibrosisRoshni MonteiroKaveri HallikeriArchana SudhakaranObjectives: The diagnostic role and correlation between phosphatase and tensin homologue and alpha-smooth muscle actin in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis was analysed by this case control study. The mechanism by which phosphatase and tensin homologue controls myofibroblast expression was also evaluated. Material and Methods: Overall, 10 normal mucosa, 30 oral submucous fibrosis (OSF) and 30 oral squamous cell carcinoma (OSCC) with OSF were stained immunohistochemically with phosphatase and tensin homologue (PTEN) and alpha-smooth muscle actin (α-SMA). Percentage positivity, pattern of expression was statistically compared using Pearson’s Chi-square and Fischer exact tests. The correlation between markers was analysed using Spearman correlation. Results: OSF and OSCC affected males predominantly with majority below 40 years and above 40 years of age respectively. Percentage of PTEN positive cells was statistically significant with gender (P = 0.024) and α-SMA distribution of pattern showed a significant correlation with habits (P = 0.018). A significant decrease in nuclear PTEN positivity (P < 0.001) and a gradual increase in α-SMA cytoplasmic expression was noted from NM to OSF and OSCC. A statistically significant weak inverse correlation existed between PTEN and α-SMA. Conclusions: A reduced phosphatase and tensin homologue expression in oral submucous fibrosis makes it more prone for malignant transformation. An increase in stromal desmoplasia modifies differentiation, invasive and proliferative capacity of tumour cells. As phosphatase and tensin homologue functions through P-Akt pathway, P-Akt with phosphatase and tensin homologue could be a therapeutic target.https://www.ejomr.org/JOMR/archives/2021/1/e3/v12n1e3ht.htmimmunohistochemistrymyofibroblastoral canceroral submucous fibrosisprognosistumor suppressor gene |
| spellingShingle | Roshni Monteiro Kaveri Hallikeri Archana Sudhakaran PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis eJournal of Oral Maxillofacial Research immunohistochemistry myofibroblast oral cancer oral submucous fibrosis prognosis tumor suppressor gene |
| title | PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis |
| title_full | PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis |
| title_fullStr | PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis |
| title_full_unstemmed | PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis |
| title_short | PTEN and α-SMA Expression and Diagnostic Role in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma with Concomitant Oral Submucous Fibrosis |
| title_sort | pten and α sma expression and diagnostic role in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis |
| topic | immunohistochemistry myofibroblast oral cancer oral submucous fibrosis prognosis tumor suppressor gene |
| url | https://www.ejomr.org/JOMR/archives/2021/1/e3/v12n1e3ht.htm |
| work_keys_str_mv | AT roshnimonteiro ptenandasmaexpressionanddiagnosticroleinoralsubmucousfibrosisandoralsquamouscellcarcinomawithconcomitantoralsubmucousfibrosis AT kaverihallikeri ptenandasmaexpressionanddiagnosticroleinoralsubmucousfibrosisandoralsquamouscellcarcinomawithconcomitantoralsubmucousfibrosis AT archanasudhakaran ptenandasmaexpressionanddiagnosticroleinoralsubmucousfibrosisandoralsquamouscellcarcinomawithconcomitantoralsubmucousfibrosis |