Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration
The ultimate goal for skin tissue engineering is to regenerate skin lesions to allow the full restoration of morphological and functional properties as what they were before injury. To this end, we have assembled a new prototype of a biomimetic human umbilical cord adult mesenchymal stem cell (hUCMS...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/1472642 |
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| author | Pia Montanucci Camilla di Pasquali Ivana Ferri Teresa Pescara Ilaria Pennoni Paola Siccu Angelo Sidoni Valerio Cervelli Giuseppe Basta Riccardo Calafiore |
| author_facet | Pia Montanucci Camilla di Pasquali Ivana Ferri Teresa Pescara Ilaria Pennoni Paola Siccu Angelo Sidoni Valerio Cervelli Giuseppe Basta Riccardo Calafiore |
| author_sort | Pia Montanucci |
| collection | DOAJ |
| description | The ultimate goal for skin tissue engineering is to regenerate skin lesions to allow the full restoration of morphological and functional properties as what they were before injury. To this end, we have assembled a new prototype of a biomimetic human umbilical cord adult mesenchymal stem cell (hUCMS)/fibrin-based scaffold. We have fully characterized the proposed dermal equivalent (DE) in vitro, to assess morphological, functional, and biological properties of the encased cells. We transplanted DE subcutaneously into immunocompetent rodents, to verify its full biocompatibility. Finally, we studied DE graft effects on full-thickness wounds, in immunocompetent mice to demonstrate its capability to drive the healing process in the absence of significant scarring tissue. The excellent outcome of these in vivo studies fuels hope that this new approach, based on a biohybrid DE, may be applied to the operative treatment of skin lesions (i.e., diabetic foot ulcers and burns) in man. |
| format | Article |
| id | doaj-art-b285297a4f5d4e259d3dd649fbc2775f |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b285297a4f5d4e259d3dd649fbc2775f2025-08-20T02:23:18ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/14726421472642Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin RegenerationPia Montanucci0Camilla di Pasquali1Ivana Ferri2Teresa Pescara3Ilaria Pennoni4Paola Siccu5Angelo Sidoni6Valerio Cervelli7Giuseppe Basta8Riccardo Calafiore9Department of Medicine, Section of Cardiovascular, Endocrine and Metabolic Clinical Physiology and Laboratory for Endocrine Cell Transplants and Biohybrid Organs, University of Perugia, Piazza Lucio Severi, Perugia, ItalyDepartment of Plastic and Reconstructive Surgery, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyDepartment of Experimental Medicine, Section of Pathologic Anatomy and Histology Medical School, University of Perugia, Perugia, ItalyDepartment of Medicine, Section of Cardiovascular, Endocrine and Metabolic Clinical Physiology and Laboratory for Endocrine Cell Transplants and Biohybrid Organs, University of Perugia, Piazza Lucio Severi, Perugia, ItalyDepartment of Medicine, Section of Cardiovascular, Endocrine and Metabolic Clinical Physiology and Laboratory for Endocrine Cell Transplants and Biohybrid Organs, University of Perugia, Piazza Lucio Severi, Perugia, ItalyDepartment of Experimental Medicine, Section of Pathologic Anatomy and Histology Medical School, University of Perugia, Perugia, ItalyDepartment of Experimental Medicine, Section of Pathologic Anatomy and Histology Medical School, University of Perugia, Perugia, ItalyDepartment of Plastic and Reconstructive Surgery, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyDepartment of Medicine, Section of Cardiovascular, Endocrine and Metabolic Clinical Physiology and Laboratory for Endocrine Cell Transplants and Biohybrid Organs, University of Perugia, Piazza Lucio Severi, Perugia, ItalyDepartment of Medicine, Section of Cardiovascular, Endocrine and Metabolic Clinical Physiology and Laboratory for Endocrine Cell Transplants and Biohybrid Organs, University of Perugia, Piazza Lucio Severi, Perugia, ItalyThe ultimate goal for skin tissue engineering is to regenerate skin lesions to allow the full restoration of morphological and functional properties as what they were before injury. To this end, we have assembled a new prototype of a biomimetic human umbilical cord adult mesenchymal stem cell (hUCMS)/fibrin-based scaffold. We have fully characterized the proposed dermal equivalent (DE) in vitro, to assess morphological, functional, and biological properties of the encased cells. We transplanted DE subcutaneously into immunocompetent rodents, to verify its full biocompatibility. Finally, we studied DE graft effects on full-thickness wounds, in immunocompetent mice to demonstrate its capability to drive the healing process in the absence of significant scarring tissue. The excellent outcome of these in vivo studies fuels hope that this new approach, based on a biohybrid DE, may be applied to the operative treatment of skin lesions (i.e., diabetic foot ulcers and burns) in man.http://dx.doi.org/10.1155/2017/1472642 |
| spellingShingle | Pia Montanucci Camilla di Pasquali Ivana Ferri Teresa Pescara Ilaria Pennoni Paola Siccu Angelo Sidoni Valerio Cervelli Giuseppe Basta Riccardo Calafiore Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration Stem Cells International |
| title | Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration |
| title_full | Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration |
| title_fullStr | Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration |
| title_full_unstemmed | Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration |
| title_short | Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration |
| title_sort | human umbilical cord wharton jelly derived adult mesenchymal stem cells in biohybrid scaffolds for experimental skin regeneration |
| url | http://dx.doi.org/10.1155/2017/1472642 |
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