Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis

This study employed a multi-omics approach to examine the impact of feed fermentation on the lipid profile and volatile flavor of duck eggs. Sensory evaluation and GC × GC-TOFMS analyses demonstrated that microbial fermentation in feed significantly reduced the off-odor of duck eggs. Among the thirt...

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Main Authors: Qishan Dong, Lizhi Lu, Tiantian Gu, Yong Tian, Yibo Zong, Jing Sun, Yu Huang, Qiuling Fu, Jun He, Tao Zeng
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525004006
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author Qishan Dong
Lizhi Lu
Tiantian Gu
Yong Tian
Yibo Zong
Jing Sun
Yu Huang
Qiuling Fu
Jun He
Tao Zeng
author_facet Qishan Dong
Lizhi Lu
Tiantian Gu
Yong Tian
Yibo Zong
Jing Sun
Yu Huang
Qiuling Fu
Jun He
Tao Zeng
author_sort Qishan Dong
collection DOAJ
description This study employed a multi-omics approach to examine the impact of feed fermentation on the lipid profile and volatile flavor of duck eggs. Sensory evaluation and GC × GC-TOFMS analyses demonstrated that microbial fermentation in feed significantly reduced the off-odor of duck eggs. Among the thirty-nine differential volatile compounds identified, six—namely 3-methylbutanal, 1-octen-3-ol, hexanal, acetophenone, 2-heptanone, and 2-pentylfuran—were likely responsible for the alteration in yolk aroma. Lipidomics and metabolomics identified twenty-five key differential lipids (p < 0.05, VIP > 1.85) and a modified metabolic pathway associated with linoleic acid (LA), involving four metabolites. Correlation analysis revealed significant associations between LA-containing lipids (e.g., DG (18: 2/18:2)), LA metabolites, and differential volatiles (e.g., 3-methylbutanal) (p < 0.05). These results provide insights into the mechanisms underlying off-odor reduction and offer a potential strategy for enhancing the flavor profile of duck eggs.
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series Food Chemistry: X
spelling doaj-art-b28168fe416d4c729fde1f0ab0c4b53c2025-08-20T03:10:31ZengElsevierFood Chemistry: X2590-15752025-05-012810255310.1016/j.fochx.2025.102553Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysisQishan Dong0Lizhi Lu1Tiantian Gu2Yong Tian3Yibo Zong4Jing Sun5Yu Huang6Qiuling Fu7Jun He8Tao Zeng9State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; College of Food Science and Engineering, Ningbo University, Ningbo 315832, PR ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Zhejiang Provincial Key Laboratory of Livestock and Poultry Biotech Breeding, Zhejiang Provincial Engineering Research Center for Poultry Breeding Industry and Green Farming Technology, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Zhejiang Provincial Key Laboratory of Livestock and Poultry Biotech Breeding, Zhejiang Provincial Engineering Research Center for Poultry Breeding Industry and Green Farming Technology, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Zhejiang Provincial Key Laboratory of Livestock and Poultry Biotech Breeding, Zhejiang Provincial Engineering Research Center for Poultry Breeding Industry and Green Farming Technology, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Zhejiang Provincial Key Laboratory of Livestock and Poultry Biotech Breeding, Zhejiang Provincial Engineering Research Center for Poultry Breeding Industry and Green Farming Technology, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR ChinaInstitute of Animal Husbandry and Vetervinary, Hubei Academy of Agricultural Science, Wuhan 430064, PR ChinaInstitute of Animal Husbandry and Vetervinary, Fujian Academy of Agricultural Science, Fuzhou 350013, PR ChinaInstitute of Animal Husbandry and Vetervinary, Fujian Academy of Agricultural Science, Fuzhou 350013, PR ChinaCollege of Food Science and Engineering, Ningbo University, Ningbo 315832, PR China; Corresponding author.State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Zhejiang Provincial Key Laboratory of Livestock and Poultry Biotech Breeding, Zhejiang Provincial Engineering Research Center for Poultry Breeding Industry and Green Farming Technology, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Corresponding author at: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.This study employed a multi-omics approach to examine the impact of feed fermentation on the lipid profile and volatile flavor of duck eggs. Sensory evaluation and GC × GC-TOFMS analyses demonstrated that microbial fermentation in feed significantly reduced the off-odor of duck eggs. Among the thirty-nine differential volatile compounds identified, six—namely 3-methylbutanal, 1-octen-3-ol, hexanal, acetophenone, 2-heptanone, and 2-pentylfuran—were likely responsible for the alteration in yolk aroma. Lipidomics and metabolomics identified twenty-five key differential lipids (p < 0.05, VIP > 1.85) and a modified metabolic pathway associated with linoleic acid (LA), involving four metabolites. Correlation analysis revealed significant associations between LA-containing lipids (e.g., DG (18: 2/18:2)), LA metabolites, and differential volatiles (e.g., 3-methylbutanal) (p < 0.05). These results provide insights into the mechanisms underlying off-odor reduction and offer a potential strategy for enhancing the flavor profile of duck eggs.http://www.sciencedirect.com/science/article/pii/S2590157525004006Volatile flavorDuck eggsLipidomicsFermented feedOff-odor
spellingShingle Qishan Dong
Lizhi Lu
Tiantian Gu
Yong Tian
Yibo Zong
Jing Sun
Yu Huang
Qiuling Fu
Jun He
Tao Zeng
Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
Food Chemistry: X
Volatile flavor
Duck eggs
Lipidomics
Fermented feed
Off-odor
title Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
title_full Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
title_fullStr Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
title_full_unstemmed Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
title_short Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
title_sort influence of fermented feed on lipid composition and volatile flavor profile of duck eggs insights from multi omics analysis
topic Volatile flavor
Duck eggs
Lipidomics
Fermented feed
Off-odor
url http://www.sciencedirect.com/science/article/pii/S2590157525004006
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