Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>

Temperature is a key environmental factor that influences mosquito phenotypic traits and the effectiveness of vector control strategies. <i>Bacillus velezensis</i> (<i>Bv</i>) has shown promise as a microbial biocontrol agent due to its insecticidal properties; however, its e...

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Main Author: Abdullah A. Alomar
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Biology
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Online Access:https://www.mdpi.com/2079-7737/14/4/357
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author Abdullah A. Alomar
author_facet Abdullah A. Alomar
author_sort Abdullah A. Alomar
collection DOAJ
description Temperature is a key environmental factor that influences mosquito phenotypic traits and the effectiveness of vector control strategies. <i>Bacillus velezensis</i> (<i>Bv</i>) has shown promise as a microbial biocontrol agent due to its insecticidal properties; however, its effects on mosquitoes under different environmental conditions are still unexplored. This study investigated the effects of <i>Bv</i> (strain WHk23) exposure on the life history traits of <i>Culex quinquefasciatus</i> at two temperature conditions (20 °C and 30 °C), focusing on development, longevity, and reproductive fitness. Results showed that temperature significantly affected mosquito development and longevity, with faster development and shorter adult longevity observed at 30 °C compared to 20 °C. Exposure to <i>Bv</i> further accelerated larval development and reduced adult emergence, with the effects being more pronounced at 30 °C than at 20 °C. Exposure to <i>Bv</i> reduced adult longevity regardless of temperature. In addition, <i>Bv</i>-exposed females had larger body sizes but lower fecundity and fertility, suggesting that <i>Bv</i> exposure may cause physiological stress that disrupts reproductive processes. These findings highlight the importance of considering environmental factors in mosquito control programs while reinforcing the efficacy of <i>Bv</i> as a sustainable biocontrol agent under a variety of environmental conditions.
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spelling doaj-art-b27f672c8c734436bb9d0cacd5e216be2025-08-20T02:17:24ZengMDPI AGBiology2079-77372025-03-0114435710.3390/biology14040357Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>Abdullah A. Alomar0Infectious Disease Vector Research Laboratory, Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaTemperature is a key environmental factor that influences mosquito phenotypic traits and the effectiveness of vector control strategies. <i>Bacillus velezensis</i> (<i>Bv</i>) has shown promise as a microbial biocontrol agent due to its insecticidal properties; however, its effects on mosquitoes under different environmental conditions are still unexplored. This study investigated the effects of <i>Bv</i> (strain WHk23) exposure on the life history traits of <i>Culex quinquefasciatus</i> at two temperature conditions (20 °C and 30 °C), focusing on development, longevity, and reproductive fitness. Results showed that temperature significantly affected mosquito development and longevity, with faster development and shorter adult longevity observed at 30 °C compared to 20 °C. Exposure to <i>Bv</i> further accelerated larval development and reduced adult emergence, with the effects being more pronounced at 30 °C than at 20 °C. Exposure to <i>Bv</i> reduced adult longevity regardless of temperature. In addition, <i>Bv</i>-exposed females had larger body sizes but lower fecundity and fertility, suggesting that <i>Bv</i> exposure may cause physiological stress that disrupts reproductive processes. These findings highlight the importance of considering environmental factors in mosquito control programs while reinforcing the efficacy of <i>Bv</i> as a sustainable biocontrol agent under a variety of environmental conditions.https://www.mdpi.com/2079-7737/14/4/357<i>Bacillus velezensis</i><i>Culex quinquefasciatus</i>biological controltemperature effectslarval developmentreproductive fitness
spellingShingle Abdullah A. Alomar
Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>
Biology
<i>Bacillus velezensis</i>
<i>Culex quinquefasciatus</i>
biological control
temperature effects
larval development
reproductive fitness
title Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>
title_full Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>
title_fullStr Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>
title_full_unstemmed Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>
title_short Effects of Temperature and <i>Bacillus velezensis</i> on the Development, Longevity, and Reproduction of <i>Culex quinquefasciatus</i>
title_sort effects of temperature and i bacillus velezensis i on the development longevity and reproduction of i culex quinquefasciatus i
topic <i>Bacillus velezensis</i>
<i>Culex quinquefasciatus</i>
biological control
temperature effects
larval development
reproductive fitness
url https://www.mdpi.com/2079-7737/14/4/357
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