The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line
Background/Objectives: This study evaluated changes in circadian clock genes and mitochondrial function in a lead (Pb)-induced toxicity model of an olfactory epithelial cell line. Methods: The DBC1.2 olfactory dark basal cell line was used. Dexamethasone shock was used to reset the circadian clock 2...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/2073-4409/14/2/81 |
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| author | Teru Kamogashira Shu Kikuta Tatsuya Yamasoba |
| author_facet | Teru Kamogashira Shu Kikuta Tatsuya Yamasoba |
| author_sort | Teru Kamogashira |
| collection | DOAJ |
| description | Background/Objectives: This study evaluated changes in circadian clock genes and mitochondrial function in a lead (Pb)-induced toxicity model of an olfactory epithelial cell line. Methods: The DBC1.2 olfactory dark basal cell line was used. Dexamethasone shock was used to reset the circadian clock 24 h (Group 1) and 36 h (Group 2) after seeding. Then, 60 h after seeding, the cells were treated with or without Pb (II) nitrate in HEPES buffer for 1 h. Mitochondrial function and cell viability were evaluated 84 h after seeding. Results: Mitochondrial function under Pb exposure was significantly impaired in Group 1 compared with Group 2. Cell numbers and viability did not significantly differ between groups. The mitochondrial membrane potential was significantly higher in Group 1 than Group 2, both without and with Pb exposure. Conclusions: The circadian rhythm can alter the sensitivity to Pb-induced toxicity and mitochondrial damage in olfactory cells. |
| format | Article |
| id | doaj-art-b2779eb0a48f4840abc5684bd6f70669 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-b2779eb0a48f4840abc5684bd6f706692025-01-24T13:26:36ZengMDPI AGCells2073-44092025-01-011428110.3390/cells14020081The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell LineTeru Kamogashira0Shu Kikuta1Tatsuya Yamasoba2Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo 113-8655, JapanDepartment of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, Nihon University, Tokyo 173-8610, JapanDepartment of Otolaryngology, Tokyo Teishin Hospital, Tokyo 102-0071, JapanBackground/Objectives: This study evaluated changes in circadian clock genes and mitochondrial function in a lead (Pb)-induced toxicity model of an olfactory epithelial cell line. Methods: The DBC1.2 olfactory dark basal cell line was used. Dexamethasone shock was used to reset the circadian clock 24 h (Group 1) and 36 h (Group 2) after seeding. Then, 60 h after seeding, the cells were treated with or without Pb (II) nitrate in HEPES buffer for 1 h. Mitochondrial function and cell viability were evaluated 84 h after seeding. Results: Mitochondrial function under Pb exposure was significantly impaired in Group 1 compared with Group 2. Cell numbers and viability did not significantly differ between groups. The mitochondrial membrane potential was significantly higher in Group 1 than Group 2, both without and with Pb exposure. Conclusions: The circadian rhythm can alter the sensitivity to Pb-induced toxicity and mitochondrial damage in olfactory cells.https://www.mdpi.com/2073-4409/14/2/81leadPbolfactory epitheliumcircadian rhythmmitochondrial function |
| spellingShingle | Teru Kamogashira Shu Kikuta Tatsuya Yamasoba The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line Cells lead Pb olfactory epithelium circadian rhythm mitochondrial function |
| title | The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line |
| title_full | The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line |
| title_fullStr | The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line |
| title_full_unstemmed | The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line |
| title_short | The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line |
| title_sort | effects of circadian rhythm on lead induced toxicity in the dbc1 2 olfactory dark basal cell line |
| topic | lead Pb olfactory epithelium circadian rhythm mitochondrial function |
| url | https://www.mdpi.com/2073-4409/14/2/81 |
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