Genomic insights into monensin resistance development in Eimeria tenella
IntroductionMonensin resistance in Eimeria tenella poses a significant challenge in poultry farming, compromising the effectiveness of this widely used anticoccidial drug. The present study aimed to identify candidate mutated genes in Eimeria tenella associated with monensin resistance through exper...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1459791/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850263437745061888 |
|---|---|
| author | Xiaolong Gu Xiaolong Gu Sufang Fang Hongbin Liu Yubo Shi Yubo Shi Yuanyuan Zhang Peng Wang Ping Cui Xinming Tang |
| author_facet | Xiaolong Gu Xiaolong Gu Sufang Fang Hongbin Liu Yubo Shi Yubo Shi Yuanyuan Zhang Peng Wang Ping Cui Xinming Tang |
| author_sort | Xiaolong Gu |
| collection | DOAJ |
| description | IntroductionMonensin resistance in Eimeria tenella poses a significant challenge in poultry farming, compromising the effectiveness of this widely used anticoccidial drug. The present study aimed to identify candidate mutated genes in Eimeria tenella associated with monensin resistance through experimental evolution and pooled genome sequencing.MethodsThe monensin-resistant (MR) strains were rapidly generated by 6 generations of serial passage under gradient monensin treatments using Houghton strain as the parental strain. Genomic sequencing was applied to uncover genetic changes during passages under drug selective pressure. Comparative analysis between resistant and control populations was performed by using the ΔSNP-index and FST values to identify loci with significant selective sweeps. Stringent thresholds were applied to pinpoint candidate genes, followed by annotation and analysis of their potential functions.Results and discussionThe genetic diversity of MR parasites remained stable across generations, despite varying drug concentrations. Seven candidate genes with 11 missense mutations were identified in MR strains. Key genes include ETH2_0729200 (dynein motor protein), ETH2_0729400 (esterase/lipase), and ETH2_0730000 (pyridine nucleotide-disulfide oxidoreductase) annotated in both the selective sweeps by using ΔSNP-index and FST methods. Further experimental validation of these candidate genes is essential to elucidate their roles in monensin resistance. This research contributes valuable insights into the molecular basis of resistance pressure in Eimeria parasites, potentially informing future strategies for the control of coccidiosis. |
| format | Article |
| id | doaj-art-b26f7b426b5240d1b7ee73da646d4f2b |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-b26f7b426b5240d1b7ee73da646d4f2b2025-08-20T01:54:57ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-04-011210.3389/fvets.2025.14597911459791Genomic insights into monensin resistance development in Eimeria tenellaXiaolong Gu0Xiaolong Gu1Sufang Fang2Hongbin Liu3Yubo Shi4Yubo Shi5Yuanyuan Zhang6Peng Wang7Ping Cui8Xinming Tang9College of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaSchool of Pharmacy, Hebei North University, Zhangjiakou, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaKey Laboratory of Animal Biosafety Risk Prevention and Control (North) and Key Laboratory of Veterinary Biological Products and Chemical Drugs of MARA, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Biological Sciences, China Agricultural University, Beijing, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaKey Laboratory of Animal Biosafety Risk Prevention and Control (North) and Key Laboratory of Veterinary Biological Products and Chemical Drugs of MARA, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaIntroductionMonensin resistance in Eimeria tenella poses a significant challenge in poultry farming, compromising the effectiveness of this widely used anticoccidial drug. The present study aimed to identify candidate mutated genes in Eimeria tenella associated with monensin resistance through experimental evolution and pooled genome sequencing.MethodsThe monensin-resistant (MR) strains were rapidly generated by 6 generations of serial passage under gradient monensin treatments using Houghton strain as the parental strain. Genomic sequencing was applied to uncover genetic changes during passages under drug selective pressure. Comparative analysis between resistant and control populations was performed by using the ΔSNP-index and FST values to identify loci with significant selective sweeps. Stringent thresholds were applied to pinpoint candidate genes, followed by annotation and analysis of their potential functions.Results and discussionThe genetic diversity of MR parasites remained stable across generations, despite varying drug concentrations. Seven candidate genes with 11 missense mutations were identified in MR strains. Key genes include ETH2_0729200 (dynein motor protein), ETH2_0729400 (esterase/lipase), and ETH2_0730000 (pyridine nucleotide-disulfide oxidoreductase) annotated in both the selective sweeps by using ΔSNP-index and FST methods. Further experimental validation of these candidate genes is essential to elucidate their roles in monensin resistance. This research contributes valuable insights into the molecular basis of resistance pressure in Eimeria parasites, potentially informing future strategies for the control of coccidiosis.https://www.frontiersin.org/articles/10.3389/fvets.2025.1459791/fullEimeria tenellamonensinresistance pressureexperimental evolutiongenome resequencing |
| spellingShingle | Xiaolong Gu Xiaolong Gu Sufang Fang Hongbin Liu Yubo Shi Yubo Shi Yuanyuan Zhang Peng Wang Ping Cui Xinming Tang Genomic insights into monensin resistance development in Eimeria tenella Frontiers in Veterinary Science Eimeria tenella monensin resistance pressure experimental evolution genome resequencing |
| title | Genomic insights into monensin resistance development in Eimeria tenella |
| title_full | Genomic insights into monensin resistance development in Eimeria tenella |
| title_fullStr | Genomic insights into monensin resistance development in Eimeria tenella |
| title_full_unstemmed | Genomic insights into monensin resistance development in Eimeria tenella |
| title_short | Genomic insights into monensin resistance development in Eimeria tenella |
| title_sort | genomic insights into monensin resistance development in eimeria tenella |
| topic | Eimeria tenella monensin resistance pressure experimental evolution genome resequencing |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1459791/full |
| work_keys_str_mv | AT xiaolonggu genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT xiaolonggu genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT sufangfang genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT hongbinliu genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT yuboshi genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT yuboshi genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT yuanyuanzhang genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT pengwang genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT pingcui genomicinsightsintomonensinresistancedevelopmentineimeriatenella AT xinmingtang genomicinsightsintomonensinresistancedevelopmentineimeriatenella |