The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell
This study investigated the differences of calcium transport-related proteins and metabolites in the uterus of hens with different breaking strength eggshell during the eggshell calcification. A total of 200 Hy-Line Brown laying hens, aged 75 weeks, were selected and categorized into two groups base...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125003505 |
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| author | Ying-ying Yang Dong Dai Hai-jun Zhang Shu-geng Wu Guang-hai Qi Jing Wang |
| author_facet | Ying-ying Yang Dong Dai Hai-jun Zhang Shu-geng Wu Guang-hai Qi Jing Wang |
| author_sort | Ying-ying Yang |
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| description | This study investigated the differences of calcium transport-related proteins and metabolites in the uterus of hens with different breaking strength eggshell during the eggshell calcification. A total of 200 Hy-Line Brown laying hens, aged 75 weeks, were selected and categorized into two groups based on the eggshell breaking strength: a high-strength group (HS, > 42 N) and a low-strength group (LS, < 32 N). Laying hens were sampled at 1 h, 7 h (the initiation stage of eggshell calcification), and 17 h (the growth stage of eggshell calcification) post-oviposition (PO). The LS group showed a decreased thickness, weight and weight ratio of eggshells, accompanied with ultrastructural deterioration and total Ca reduction. The expression levels of ATP2A3, ATP2B2, SLC8A1, and SLC8A3 were significantly increased in the HS at 17 h PO when compared to 1 h and 7 h PO, while no significant changes were observed in the LS. At 7 h PO, the LS group had lower uterine mucosa calcium levels, higher TRPV6 protein expression, and lower CALB1 protein expression. In the HS group, uterine metabolites showed a significant increase in glutathione, citrulline, and proline at 7 h PO, whereas, at 17 h PO, the tricarboxylic acid cycle pathway was significantly enriched. These findings suggest that uterine calcium transport activity is relatively subdued during the initiation stage of eggshell calcification, focusing on redox repair activities to maintain homeostasis for mammillary knobs formation. Subsequently, uterine calcium transport activity becomes highly active during the growth stage of eggshell calcification, primarily supporting rapid calcium transport through enhanced energy metabolism. In aged laying hens, the lower eggshell breaking strength may be attributed to decreased calcium levels during the initiation stage and imbalanced redox during the growth stage, which could affect calcium transport and lead to a weak ultrastructure during the calcification period. |
| format | Article |
| id | doaj-art-b261b248f2f44226bca93910d88e9d90 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-b261b248f2f44226bca93910d88e9d902025-08-20T03:24:47ZengElsevierPoultry Science0032-57912025-06-01104610511110.1016/j.psj.2025.105111The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshellYing-ying Yang0Dong Dai1Hai-jun Zhang2Shu-geng Wu3Guang-hai Qi4Jing Wang5Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR ChinaLaboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR ChinaLaboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR ChinaLaboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR ChinaLaboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR ChinaCorresponding author at: Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR China.; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, PR ChinaThis study investigated the differences of calcium transport-related proteins and metabolites in the uterus of hens with different breaking strength eggshell during the eggshell calcification. A total of 200 Hy-Line Brown laying hens, aged 75 weeks, were selected and categorized into two groups based on the eggshell breaking strength: a high-strength group (HS, > 42 N) and a low-strength group (LS, < 32 N). Laying hens were sampled at 1 h, 7 h (the initiation stage of eggshell calcification), and 17 h (the growth stage of eggshell calcification) post-oviposition (PO). The LS group showed a decreased thickness, weight and weight ratio of eggshells, accompanied with ultrastructural deterioration and total Ca reduction. The expression levels of ATP2A3, ATP2B2, SLC8A1, and SLC8A3 were significantly increased in the HS at 17 h PO when compared to 1 h and 7 h PO, while no significant changes were observed in the LS. At 7 h PO, the LS group had lower uterine mucosa calcium levels, higher TRPV6 protein expression, and lower CALB1 protein expression. In the HS group, uterine metabolites showed a significant increase in glutathione, citrulline, and proline at 7 h PO, whereas, at 17 h PO, the tricarboxylic acid cycle pathway was significantly enriched. These findings suggest that uterine calcium transport activity is relatively subdued during the initiation stage of eggshell calcification, focusing on redox repair activities to maintain homeostasis for mammillary knobs formation. Subsequently, uterine calcium transport activity becomes highly active during the growth stage of eggshell calcification, primarily supporting rapid calcium transport through enhanced energy metabolism. In aged laying hens, the lower eggshell breaking strength may be attributed to decreased calcium levels during the initiation stage and imbalanced redox during the growth stage, which could affect calcium transport and lead to a weak ultrastructure during the calcification period.http://www.sciencedirect.com/science/article/pii/S0032579125003505Calcium transportEggshell calcification stageEggshell qualityLaying henMetabolomics |
| spellingShingle | Ying-ying Yang Dong Dai Hai-jun Zhang Shu-geng Wu Guang-hai Qi Jing Wang The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell Poultry Science Calcium transport Eggshell calcification stage Eggshell quality Laying hen Metabolomics |
| title | The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell |
| title_full | The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell |
| title_fullStr | The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell |
| title_full_unstemmed | The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell |
| title_short | The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell |
| title_sort | characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell |
| topic | Calcium transport Eggshell calcification stage Eggshell quality Laying hen Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125003505 |
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