Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains

Summary: We present a protocol to produce electrocompetent Acetobacterium woodii DSM 1030 and Eubacterium callanderi DSM 3468 cells. These acetogens are capable of converting CO2 with H2 and other C1 substrates into organic acids. We describe the electroporation procedure and the verification steps...

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Bibliographic Details
Main Authors: Kira Sofie Baur, Barbara Rühle, Tabea Reith, Anica Krieg, Frank Robert Bengelsdorf
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004174
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