Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains
Summary: We present a protocol to produce electrocompetent Acetobacterium woodii DSM 1030 and Eubacterium callanderi DSM 3468 cells. These acetogens are capable of converting CO2 with H2 and other C1 substrates into organic acids. We describe the electroporation procedure and the verification steps...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004174 |
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| author | Kira Sofie Baur Barbara Rühle Tabea Reith Anica Krieg Frank Robert Bengelsdorf |
| author_facet | Kira Sofie Baur Barbara Rühle Tabea Reith Anica Krieg Frank Robert Bengelsdorf |
| author_sort | Kira Sofie Baur |
| collection | DOAJ |
| description | Summary: We present a protocol to produce electrocompetent Acetobacterium woodii DSM 1030 and Eubacterium callanderi DSM 3468 cells. These acetogens are capable of converting CO2 with H2 and other C1 substrates into organic acids. We describe the electroporation procedure and the verification steps to prove the presence of recombinant strains. This protocol serves as a basis for the application of the genetic toolbox for these bacterial species and can be adapted to several anaerobic strains.For complete details on the use and execution of this protocol, please refer to Leang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-b256f4a34b194ad394fd9c5a07ddb97d |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-b256f4a34b194ad394fd9c5a07ddb97d2025-08-20T03:44:36ZengElsevierSTAR Protocols2666-16672025-09-016310401110.1016/j.xpro.2025.104011Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strainsKira Sofie Baur0Barbara Rühle1Tabea Reith2Anica Krieg3Frank Robert Bengelsdorf4Molecular Biology and Biotechnology of Prokaryotes, Ulm University, Albert-Einstein-Allee 11, 89069 Ulm, Germany; Corresponding authorMolecular Biology and Biotechnology of Prokaryotes, Ulm University, Albert-Einstein-Allee 11, 89069 Ulm, GermanyMolecular Biology and Biotechnology of Prokaryotes, Ulm University, Albert-Einstein-Allee 11, 89069 Ulm, GermanyMolecular Biology and Biotechnology of Prokaryotes, Ulm University, Albert-Einstein-Allee 11, 89069 Ulm, GermanyMolecular Biology and Biotechnology of Prokaryotes, Ulm University, Albert-Einstein-Allee 11, 89069 Ulm, GermanySummary: We present a protocol to produce electrocompetent Acetobacterium woodii DSM 1030 and Eubacterium callanderi DSM 3468 cells. These acetogens are capable of converting CO2 with H2 and other C1 substrates into organic acids. We describe the electroporation procedure and the verification steps to prove the presence of recombinant strains. This protocol serves as a basis for the application of the genetic toolbox for these bacterial species and can be adapted to several anaerobic strains.For complete details on the use and execution of this protocol, please refer to Leang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725004174MetabolismMicrobiologyMolecular Biology |
| spellingShingle | Kira Sofie Baur Barbara Rühle Tabea Reith Anica Krieg Frank Robert Bengelsdorf Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains STAR Protocols Metabolism Microbiology Molecular Biology |
| title | Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains |
| title_full | Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains |
| title_fullStr | Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains |
| title_full_unstemmed | Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains |
| title_short | Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains |
| title_sort | protocol to obtain genetically engineered acetobacterium woodii and eubacterium callanderi strains |
| topic | Metabolism Microbiology Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725004174 |
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