Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production

The efficient growth of microalgae in sewage wastewater presents a sustainable approach for the recovery of valuable products, such as lipids and hydrocarbons. This study focuses on bench-scale cultivation and optimization to enhance the growth rates and biomass productivity of Scenedesmus sp., Chlo...

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Main Authors: Nikita Singh, Sudesh Rathilal
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2024-11-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/14864
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author Nikita Singh
Sudesh Rathilal
author_facet Nikita Singh
Sudesh Rathilal
author_sort Nikita Singh
collection DOAJ
description The efficient growth of microalgae in sewage wastewater presents a sustainable approach for the recovery of valuable products, such as lipids and hydrocarbons. This study focuses on bench-scale cultivation and optimization to enhance the growth rates and biomass productivity of Scenedesmus sp., Chlorella vulgaris, and Spirulina platensis. The performance of bench-scale reactors under controlled conditions was investigated to maximize algal growth and biomass productivity. The objective of this study employed a series of bench-scale experiments populated using Box-Behnken in Design Expert, Design of Experiments (DOE) to identify optimal configurations. Key parameters, including pH (6.5 – 10), illumination period of light: dark (0:24, 6:18, 12:12. 18:6, 24:0), and growth medium to wastewater ratio of (0:4, 1:3, 2:2, 3:1, 4:0) were systematically varied to assess their effects on microalgal growth. The results demonstrated that specific conditions significantly influenced algal growth and overall lipid productivity. Using Bold’s Basal Medium (BBM) for cultivation, Scenedesmus sp. achieved the highest growth rate of 0.86/d with biomass productivity of 39 × 104 cells/mL/d. Chlorella vulgaris performed best with a growth rate of 0.434/d and 24.38 × 104 cells/mL/d. Spirulina platensis maximum growth rate achieved 0.268/d and 23.36 × 104 cells/mL/d. Bench scale experiments further identified and incorporated optimal cultivation conditions, achieving higher growth rates and biomass productivity. These findings demonstrated the potential of optimized bench-scale reactors, providing a scalable solution for biofuel production using bubble columns, airlift reactors and industrial applications. This research contributes to the development of sustainable biofuel production technologies aligning with global efforts to reduce environmental impact and promote renewable energy sources.
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spelling doaj-art-b25269c6353d470782a46dd2b9f4ae752025-08-20T02:07:13ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162024-11-01113Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel ProductionNikita SinghSudesh RathilalThe efficient growth of microalgae in sewage wastewater presents a sustainable approach for the recovery of valuable products, such as lipids and hydrocarbons. This study focuses on bench-scale cultivation and optimization to enhance the growth rates and biomass productivity of Scenedesmus sp., Chlorella vulgaris, and Spirulina platensis. The performance of bench-scale reactors under controlled conditions was investigated to maximize algal growth and biomass productivity. The objective of this study employed a series of bench-scale experiments populated using Box-Behnken in Design Expert, Design of Experiments (DOE) to identify optimal configurations. Key parameters, including pH (6.5 – 10), illumination period of light: dark (0:24, 6:18, 12:12. 18:6, 24:0), and growth medium to wastewater ratio of (0:4, 1:3, 2:2, 3:1, 4:0) were systematically varied to assess their effects on microalgal growth. The results demonstrated that specific conditions significantly influenced algal growth and overall lipid productivity. Using Bold’s Basal Medium (BBM) for cultivation, Scenedesmus sp. achieved the highest growth rate of 0.86/d with biomass productivity of 39 × 104 cells/mL/d. Chlorella vulgaris performed best with a growth rate of 0.434/d and 24.38 × 104 cells/mL/d. Spirulina platensis maximum growth rate achieved 0.268/d and 23.36 × 104 cells/mL/d. Bench scale experiments further identified and incorporated optimal cultivation conditions, achieving higher growth rates and biomass productivity. These findings demonstrated the potential of optimized bench-scale reactors, providing a scalable solution for biofuel production using bubble columns, airlift reactors and industrial applications. This research contributes to the development of sustainable biofuel production technologies aligning with global efforts to reduce environmental impact and promote renewable energy sources.https://www.cetjournal.it/index.php/cet/article/view/14864
spellingShingle Nikita Singh
Sudesh Rathilal
Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production
Chemical Engineering Transactions
title Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production
title_full Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production
title_fullStr Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production
title_full_unstemmed Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production
title_short Cultivation of Scenedesmus sp., Chlorella vulgaris and Spirulina platensis in Sewage Industrial Wastewater: Bench-Scale Optimization for Biofuel Production
title_sort cultivation of scenedesmus sp chlorella vulgaris and spirulina platensis in sewage industrial wastewater bench scale optimization for biofuel production
url https://www.cetjournal.it/index.php/cet/article/view/14864
work_keys_str_mv AT nikitasingh cultivationofscenedesmusspchlorellavulgarisandspirulinaplatensisinsewageindustrialwastewaterbenchscaleoptimizationforbiofuelproduction
AT sudeshrathilal cultivationofscenedesmusspchlorellavulgarisandspirulinaplatensisinsewageindustrialwastewaterbenchscaleoptimizationforbiofuelproduction