Identification of Datura innoxia as a potential source of antimycobacterial components
Datura innoxia is a medicinal plant from the Solanaceae family, having medicinal properties and some toxic effects. It is widely distributed across Asia, Africa, Europe, the Americas, and other tropical and subtropical regions, where it is utilized by local pharmaceutical industries. In this study,...
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| Format: | Article |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1553282/full |
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| author | Sajjad Ahmed Khan Sajjad Ahmed Khan Sajjad Ahmed Khan Muzafar Ahmad Rather Muhammad Sheeraz Ahmad Ziyi Jia Anthony D. Baughn Sajid Iqbal Syed Mehmood Qadir Sabira Tahseen Muhammad Umer Khan Peter W. Villalta Peter W. Villalta W. Thomas Shier |
| author_facet | Sajjad Ahmed Khan Sajjad Ahmed Khan Sajjad Ahmed Khan Muzafar Ahmad Rather Muhammad Sheeraz Ahmad Ziyi Jia Anthony D. Baughn Sajid Iqbal Syed Mehmood Qadir Sabira Tahseen Muhammad Umer Khan Peter W. Villalta Peter W. Villalta W. Thomas Shier |
| author_sort | Sajjad Ahmed Khan |
| collection | DOAJ |
| description | Datura innoxia is a medicinal plant from the Solanaceae family, having medicinal properties and some toxic effects. It is widely distributed across Asia, Africa, Europe, the Americas, and other tropical and subtropical regions, where it is utilized by local pharmaceutical industries. In this study, bioassay-guided fractionation and LC-MS/MS analysis were used for the identification of secondary metabolites with anti-tuberculosis activity in methanolic leaf extracts of D. innoxia. Bioassay-guided fractionation was conducted using normal and reverse phase column chromatography, and the fractions were assayed for antituberculosis activity in vitro by serial dilution in Mycobacterium tuberculosis H37Ra cultures. The structures of known secondary metabolites in the purified extracts were identified using LC-ESI-MS/MS mass spectroscopy. A purified fraction of the methanolic extract of D. innoxia leaves inhibited M. tuberculosis growth at concentrations as low as 25 μg/mL. Metabolic profiling with LC-ESI-MS/MS enabled the identification of the purified extract of 16 known metabolites, including loliolide, scopolamine, kuromanin, isoquercitrin, moupinamide, methyl isoquinoline-3-carboxylate, trans-3-Indoleacrylic acid, tyramine, (3β,5ξ,9ξ)-3,6,19-trihydroxyurs-12-en-28-oic acid, milbemycin A3 oxime, methyl jasmonate, nicotinamide, methyl ferulate, trifolin, 2-[(1S,2S,4aR,8aS)-1-hydroxy-4a-methyl-8-methylidene-decahydronaphthalen-2-yl]prop-2-enoic acid, and methyl 4-hydroxycinnamate. These results indicate that D. innoxia is a rich natural source of potential antitubercular secondary metabolites. |
| format | Article |
| id | doaj-art-b24e2cb4bc86481f85fc41dc478aa135 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-b24e2cb4bc86481f85fc41dc478aa1352025-08-20T03:29:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15532821553282Identification of Datura innoxia as a potential source of antimycobacterial componentsSajjad Ahmed Khan0Sajjad Ahmed Khan1Sajjad Ahmed Khan2Muzafar Ahmad Rather3Muhammad Sheeraz Ahmad4Ziyi Jia5Anthony D. Baughn6Sajid Iqbal7Syed Mehmood Qadir8Sabira Tahseen9Muhammad Umer Khan10Peter W. Villalta11Peter W. Villalta12W. Thomas Shier13University Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi, PakistanNational Reference Laboratory for Tuberculosis, National TB Control Program, Islamabad, PakistanDepartment of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, United StatesDepartment of Microbiology & Immunology, University of Minnesota Medical School, Minneapolis, MN, United StatesUniversity Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi, PakistanDepartment of Microbiology & Immunology, University of Minnesota Medical School, Minneapolis, MN, United StatesDepartment of Microbiology & Immunology, University of Minnesota Medical School, Minneapolis, MN, United StatesDepartment of Microbiology, Quaid-i-Azam University, Islamabad, PakistanNational Reference Laboratory for Tuberculosis, National TB Control Program, Islamabad, PakistanNational Reference Laboratory for Tuberculosis, National TB Control Program, Islamabad, PakistanInstitute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, PakistanDepartment of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, United StatesMasonic Cancer Center, University of Minnesota, Minneapolis, MN, United StatesDepartment of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, United StatesDatura innoxia is a medicinal plant from the Solanaceae family, having medicinal properties and some toxic effects. It is widely distributed across Asia, Africa, Europe, the Americas, and other tropical and subtropical regions, where it is utilized by local pharmaceutical industries. In this study, bioassay-guided fractionation and LC-MS/MS analysis were used for the identification of secondary metabolites with anti-tuberculosis activity in methanolic leaf extracts of D. innoxia. Bioassay-guided fractionation was conducted using normal and reverse phase column chromatography, and the fractions were assayed for antituberculosis activity in vitro by serial dilution in Mycobacterium tuberculosis H37Ra cultures. The structures of known secondary metabolites in the purified extracts were identified using LC-ESI-MS/MS mass spectroscopy. A purified fraction of the methanolic extract of D. innoxia leaves inhibited M. tuberculosis growth at concentrations as low as 25 μg/mL. Metabolic profiling with LC-ESI-MS/MS enabled the identification of the purified extract of 16 known metabolites, including loliolide, scopolamine, kuromanin, isoquercitrin, moupinamide, methyl isoquinoline-3-carboxylate, trans-3-Indoleacrylic acid, tyramine, (3β,5ξ,9ξ)-3,6,19-trihydroxyurs-12-en-28-oic acid, milbemycin A3 oxime, methyl jasmonate, nicotinamide, methyl ferulate, trifolin, 2-[(1S,2S,4aR,8aS)-1-hydroxy-4a-methyl-8-methylidene-decahydronaphthalen-2-yl]prop-2-enoic acid, and methyl 4-hydroxycinnamate. These results indicate that D. innoxia is a rich natural source of potential antitubercular secondary metabolites.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1553282/fullMycobacterium tuberculosisDatura innoxiaantitubercular activityLC-MS/MSscopolaminemilbemycin A3 oxime |
| spellingShingle | Sajjad Ahmed Khan Sajjad Ahmed Khan Sajjad Ahmed Khan Muzafar Ahmad Rather Muhammad Sheeraz Ahmad Ziyi Jia Anthony D. Baughn Sajid Iqbal Syed Mehmood Qadir Sabira Tahseen Muhammad Umer Khan Peter W. Villalta Peter W. Villalta W. Thomas Shier Identification of Datura innoxia as a potential source of antimycobacterial components Frontiers in Microbiology Mycobacterium tuberculosis Datura innoxia antitubercular activity LC-MS/MS scopolamine milbemycin A3 oxime |
| title | Identification of Datura innoxia as a potential source of antimycobacterial components |
| title_full | Identification of Datura innoxia as a potential source of antimycobacterial components |
| title_fullStr | Identification of Datura innoxia as a potential source of antimycobacterial components |
| title_full_unstemmed | Identification of Datura innoxia as a potential source of antimycobacterial components |
| title_short | Identification of Datura innoxia as a potential source of antimycobacterial components |
| title_sort | identification of datura innoxia as a potential source of antimycobacterial components |
| topic | Mycobacterium tuberculosis Datura innoxia antitubercular activity LC-MS/MS scopolamine milbemycin A3 oxime |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1553282/full |
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