HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin
Persistent high-risk human papillomaviruses (HR-HPVs) infection is the leading cause of cervical cancer. With over 200 circulating genotypes, HPV detection, management, and prevention remain challenging. In Benin, HPV prevalence and genotype distribution are largely unknown, and no national HPV vacc...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/5/714 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849719454742609920 |
|---|---|
| author | Clémence D. Gouton Ifeoluwa O. Bejide Oludayo O. Ope-ewe Marius Adjagba Simon Azonbakin Gaonyadiwe Muzanywa Florence T. Akinyi Arnaud Agbanlinsou Yanique Goussanou Onikepe Folarin Anatole Laleye Christian T. Happi Chinedu A. Ugwu |
| author_facet | Clémence D. Gouton Ifeoluwa O. Bejide Oludayo O. Ope-ewe Marius Adjagba Simon Azonbakin Gaonyadiwe Muzanywa Florence T. Akinyi Arnaud Agbanlinsou Yanique Goussanou Onikepe Folarin Anatole Laleye Christian T. Happi Chinedu A. Ugwu |
| author_sort | Clémence D. Gouton |
| collection | DOAJ |
| description | Persistent high-risk human papillomaviruses (HR-HPVs) infection is the leading cause of cervical cancer. With over 200 circulating genotypes, HPV detection, management, and prevention remain challenging. In Benin, HPV prevalence and genotype distribution are largely unknown, and no national HPV vaccination program exists. This study investigates the prevalence, genotypic diversity, and risk factors of HIV–HPV co-infection among women in Cotonou, Benin. Cervical swabs were collected from 100 women living with HIV (WLWHIV) and 51 women without HIV (WWHIV) at two hospitals. DNA extraction and nested polymerase chain reaction (PCR) were used to detect HPV, followed by Sanger sequencing for genotyping. Chi-squared analysis was used to assess risk factors. HPV was detected in 85% (85/100) of WLWHIV and 60.8% (31/51) of WWHIV (<i>p</i> = 0.002), confirming HIV as an independent risk factor. Fifteen HR-HPV genotypes were identified, with HPV 45 most prevalent in WLWHIV and HPV 16 in WWHIV. Notably, HR-HPV 67, 70, and 82 were detected for the first time in Benin. Unmarried status and detectable HIV load were significant risk factors for co-infection. The high HPV prevalence, particularly among WLWHIV, underscores the urgent need for HPV surveillance and vaccination in Benin. Identifying novel HR-HPV genotypes highlights the necessity for ongoing monitoring and targeted prevention strategies. |
| format | Article |
| id | doaj-art-b24e2bfb2c1845c58deab01abdc5b6fc |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-b24e2bfb2c1845c58deab01abdc5b6fc2025-08-20T03:12:09ZengMDPI AGViruses1999-49152025-05-0117571410.3390/v17050714HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of BeninClémence D. Gouton0Ifeoluwa O. Bejide1Oludayo O. Ope-ewe2Marius Adjagba3Simon Azonbakin4Gaonyadiwe Muzanywa5Florence T. Akinyi6Arnaud Agbanlinsou7Yanique Goussanou8Onikepe Folarin9Anatole Laleye10Christian T. Happi11Chinedu A. Ugwu12The Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaLaboratory of Histology-Reproductive Biology, Cytogenetics and Medical Genetics, Human Biology Unit, Faculty of Health Sciences, Abomey-Calavi University, Cotonou 01 BP 188, BeninLaboratory of Histology-Reproductive Biology, Cytogenetics and Medical Genetics, Human Biology Unit, Faculty of Health Sciences, Abomey-Calavi University, Cotonou 01 BP 188, BeninThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaLaboratory of Histology-Reproductive Biology, Cytogenetics and Medical Genetics, Human Biology Unit, Faculty of Health Sciences, Abomey-Calavi University, Cotonou 01 BP 188, BeninLaboratory of Histology-Reproductive Biology, Cytogenetics and Medical Genetics, Human Biology Unit, Faculty of Health Sciences, Abomey-Calavi University, Cotonou 01 BP 188, BeninThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaLaboratory of Histology-Reproductive Biology, Cytogenetics and Medical Genetics, Human Biology Unit, Faculty of Health Sciences, Abomey-Calavi University, Cotonou 01 BP 188, BeninThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaThe Africa Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University Nigeria, Ede PMB 230, NigeriaPersistent high-risk human papillomaviruses (HR-HPVs) infection is the leading cause of cervical cancer. With over 200 circulating genotypes, HPV detection, management, and prevention remain challenging. In Benin, HPV prevalence and genotype distribution are largely unknown, and no national HPV vaccination program exists. This study investigates the prevalence, genotypic diversity, and risk factors of HIV–HPV co-infection among women in Cotonou, Benin. Cervical swabs were collected from 100 women living with HIV (WLWHIV) and 51 women without HIV (WWHIV) at two hospitals. DNA extraction and nested polymerase chain reaction (PCR) were used to detect HPV, followed by Sanger sequencing for genotyping. Chi-squared analysis was used to assess risk factors. HPV was detected in 85% (85/100) of WLWHIV and 60.8% (31/51) of WWHIV (<i>p</i> = 0.002), confirming HIV as an independent risk factor. Fifteen HR-HPV genotypes were identified, with HPV 45 most prevalent in WLWHIV and HPV 16 in WWHIV. Notably, HR-HPV 67, 70, and 82 were detected for the first time in Benin. Unmarried status and detectable HIV load were significant risk factors for co-infection. The high HPV prevalence, particularly among WLWHIV, underscores the urgent need for HPV surveillance and vaccination in Benin. Identifying novel HR-HPV genotypes highlights the necessity for ongoing monitoring and targeted prevention strategies.https://www.mdpi.com/1999-4915/17/5/714human papillomavirus (HPV)HIVcervical cancerHPV–HIV co-infectionrisk factorsprevalence |
| spellingShingle | Clémence D. Gouton Ifeoluwa O. Bejide Oludayo O. Ope-ewe Marius Adjagba Simon Azonbakin Gaonyadiwe Muzanywa Florence T. Akinyi Arnaud Agbanlinsou Yanique Goussanou Onikepe Folarin Anatole Laleye Christian T. Happi Chinedu A. Ugwu HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin Viruses human papillomavirus (HPV) HIV cervical cancer HPV–HIV co-infection risk factors prevalence |
| title | HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin |
| title_full | HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin |
| title_fullStr | HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin |
| title_full_unstemmed | HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin |
| title_short | HIV–HPV Co-Infection and Identification of Novel High-Risk HPV Among Women at Two Hospital Centers in Cotonou, Republic of Benin |
| title_sort | hiv hpv co infection and identification of novel high risk hpv among women at two hospital centers in cotonou republic of benin |
| topic | human papillomavirus (HPV) HIV cervical cancer HPV–HIV co-infection risk factors prevalence |
| url | https://www.mdpi.com/1999-4915/17/5/714 |
| work_keys_str_mv | AT clemencedgouton hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT ifeoluwaobejide hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT oludayooopeewe hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT mariusadjagba hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT simonazonbakin hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT gaonyadiwemuzanywa hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT florencetakinyi hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT arnaudagbanlinsou hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT yaniquegoussanou hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT onikepefolarin hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT anatolelaleye hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT christianthappi hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin AT chineduaugwu hivhpvcoinfectionandidentificationofnovelhighriskhpvamongwomenattwohospitalcentersincotonourepublicofbenin |