The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities

This study was carried out to determine the effects of lactic acid bacteria+ enzyme (LAB+E) inoculants on the fermentation characteristics and feed values of silages prepared from alfalfa harvested at three maturity stages. Alfalfa was harvested at the early, middle and late flowering stages. Sil-Al...

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Main Authors: Berrin Okuyucu, Selma Büyükkılıç Beyzi, Mehmet Levent Özdüven
Format: Article
Language:English
Published: Hasan Eleroğlu 2021-07-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/4193
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author Berrin Okuyucu
Selma Büyükkılıç Beyzi
Mehmet Levent Özdüven
author_facet Berrin Okuyucu
Selma Büyükkılıç Beyzi
Mehmet Levent Özdüven
author_sort Berrin Okuyucu
collection DOAJ
description This study was carried out to determine the effects of lactic acid bacteria+ enzyme (LAB+E) inoculants on the fermentation characteristics and feed values of silages prepared from alfalfa harvested at three maturity stages. Alfalfa was harvested at the early, middle and late flowering stages. Sil-All (Alltech, UK) were used as LAB+E inoculants. Inoculants were applied to the silages at the rates of 1×105, 5×105 and 1×106 cfu/g levels in 1 liter capacity plastic bags. The bags were stored at 20±2°C under the laboratory conditions. Three bags from each group were sampled for chemical and microbiological analyses on the 45th day after ensiling. The results showed that LAB+E inoculants reduced pH values and ammonia-nitrogen content, whereas increased lactic acid contents and lactobacillus count of alfalfa silages. High doses LAB+E inoculant decreased neutral detergent fiber and acid detergent fiber content, increased in vitro organic matter digestibility and metabolic energy of alfalfa silages. It has been demonstrated that the most effective application dose of LAB+E inoculant to improve fermentation and feed value of alfalfa silage was 1×106 cfu/g, but 1x105 and 5×105 cfu/g level can also be considered as effective dose.
format Article
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institution Kabale University
issn 2148-127X
language English
publishDate 2021-07-01
publisher Hasan Eleroğlu
record_format Article
series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-b24c8d47644f4d5c92d583d22672747c2025-08-20T03:39:26ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2021-07-01961062106910.24925/turjaf.v9i6.1062-1069.41932103The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different MaturitiesBerrin Okuyucu0Selma Büyükkılıç Beyzi1Mehmet Levent Özdüven2Department of Animal Science, Faculty of Agriculture, Tekirdağ Namık Kemal University, 59030 TekirdağDepartment of Animal Science, Seyrani Faculty of Agriculture, Erciyes University, 38039 KayseriDepartment of Animal Science, Faculty of Agriculture, Tekirdağ Namık Kemal University, 59030 TekirdağThis study was carried out to determine the effects of lactic acid bacteria+ enzyme (LAB+E) inoculants on the fermentation characteristics and feed values of silages prepared from alfalfa harvested at three maturity stages. Alfalfa was harvested at the early, middle and late flowering stages. Sil-All (Alltech, UK) were used as LAB+E inoculants. Inoculants were applied to the silages at the rates of 1×105, 5×105 and 1×106 cfu/g levels in 1 liter capacity plastic bags. The bags were stored at 20±2°C under the laboratory conditions. Three bags from each group were sampled for chemical and microbiological analyses on the 45th day after ensiling. The results showed that LAB+E inoculants reduced pH values and ammonia-nitrogen content, whereas increased lactic acid contents and lactobacillus count of alfalfa silages. High doses LAB+E inoculant decreased neutral detergent fiber and acid detergent fiber content, increased in vitro organic matter digestibility and metabolic energy of alfalfa silages. It has been demonstrated that the most effective application dose of LAB+E inoculant to improve fermentation and feed value of alfalfa silage was 1×106 cfu/g, but 1x105 and 5×105 cfu/g level can also be considered as effective dose.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4193alfalfafermentationlactic acid bacterial inoculantsaerobic stabilityfeed value
spellingShingle Berrin Okuyucu
Selma Büyükkılıç Beyzi
Mehmet Levent Özdüven
The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities
Turkish Journal of Agriculture: Food Science and Technology
alfalfa
fermentation
lactic acid bacterial inoculants
aerobic stability
feed value
title The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities
title_full The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities
title_fullStr The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities
title_full_unstemmed The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities
title_short The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities
title_sort effects of lactic acid bacteria and enzyme mixture inoculants on silage fermentation characteristics and feed values of silage prepared from alfalfa harvested at different maturities
topic alfalfa
fermentation
lactic acid bacterial inoculants
aerobic stability
feed value
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/4193
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