Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide

<i>Porphyra yezoensis</i> has attracted much attention due to its gelling properties and bioactivity. In this study, the chemical structure of <i>Porphyra yezoensis</i> polysaccharides (PYPSs) was characterized, and the effects of concentration, temperature, pH, and calcium i...

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Main Authors: Chenyang Ji, Xiaoshan Long, Jingjie Wang, Bo Qi, Yang Cao, Xiao Hu
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/4/882
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author Chenyang Ji
Xiaoshan Long
Jingjie Wang
Bo Qi
Yang Cao
Xiao Hu
author_facet Chenyang Ji
Xiaoshan Long
Jingjie Wang
Bo Qi
Yang Cao
Xiao Hu
author_sort Chenyang Ji
collection DOAJ
description <i>Porphyra yezoensis</i> has attracted much attention due to its gelling properties and bioactivity. In this study, the chemical structure of <i>Porphyra yezoensis</i> polysaccharides (PYPSs) was characterized, and the effects of concentration, temperature, pH, and calcium ion (Ca<sup>2+</sup>) addition on the rheological properties of PYPS were systematically investigated. Chemical composition analysis indicated that PYPS primarily contained galactose (89.76%) and sulfate (15.57%). Rheological tests demonstrated that PYPS exhibited typical pseudoplastic properties, with apparent viscosity increasing with an increasing concentration. Temperature elevation from 30 °C to 90 °C weakened the intermolecular forces and reduced the apparent viscosity, whereas neutral pH (7.0) provided an optimal electrostatic equilibrium to maintain the highest viscosity. Ca<sup>2+</sup> could modulate the interactions between PYPS molecules and affect the formation of the gel network structure. When the Ca<sup>2+</sup> concentration reached the optimal value of 6 mM, the calcium bridges formed between Ca<sup>2+</sup> and PYPS molecules not only enhanced the rheological behavior and textural properties but also formed a smooth and well-ordered network structure, achieving the highest value of fractal dimension (D<sub>f</sub> = 2.9600), though excessive Ca<sup>2+</sup> disrupted this well-ordered structure. Furthermore, PYPS possessed significant scavenging ability against DPPH, ABTS, and HO• radicals, demonstrating its potential application as a natural antioxidant in functional foods.
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spelling doaj-art-b24760f7a70b4e9bb9e9ad99bf3963e42025-08-20T02:03:31ZengMDPI AGMolecules1420-30492025-02-0130488210.3390/molecules30040882Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> PolysaccharideChenyang Ji0Xiaoshan Long1Jingjie Wang2Bo Qi3Yang Cao4Xiao Hu5Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Urban Agriculture in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Urban Agriculture in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China<i>Porphyra yezoensis</i> has attracted much attention due to its gelling properties and bioactivity. In this study, the chemical structure of <i>Porphyra yezoensis</i> polysaccharides (PYPSs) was characterized, and the effects of concentration, temperature, pH, and calcium ion (Ca<sup>2+</sup>) addition on the rheological properties of PYPS were systematically investigated. Chemical composition analysis indicated that PYPS primarily contained galactose (89.76%) and sulfate (15.57%). Rheological tests demonstrated that PYPS exhibited typical pseudoplastic properties, with apparent viscosity increasing with an increasing concentration. Temperature elevation from 30 °C to 90 °C weakened the intermolecular forces and reduced the apparent viscosity, whereas neutral pH (7.0) provided an optimal electrostatic equilibrium to maintain the highest viscosity. Ca<sup>2+</sup> could modulate the interactions between PYPS molecules and affect the formation of the gel network structure. When the Ca<sup>2+</sup> concentration reached the optimal value of 6 mM, the calcium bridges formed between Ca<sup>2+</sup> and PYPS molecules not only enhanced the rheological behavior and textural properties but also formed a smooth and well-ordered network structure, achieving the highest value of fractal dimension (D<sub>f</sub> = 2.9600), though excessive Ca<sup>2+</sup> disrupted this well-ordered structure. Furthermore, PYPS possessed significant scavenging ability against DPPH, ABTS, and HO• radicals, demonstrating its potential application as a natural antioxidant in functional foods.https://www.mdpi.com/1420-3049/30/4/882<i>Porphyra yezoensis</i> polysacchariderheological propertiesgelation mechanismcalcium ionsantioxidant activities
spellingShingle Chenyang Ji
Xiaoshan Long
Jingjie Wang
Bo Qi
Yang Cao
Xiao Hu
Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide
Molecules
<i>Porphyra yezoensis</i> polysaccharide
rheological properties
gelation mechanism
calcium ions
antioxidant activities
title Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide
title_full Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide
title_fullStr Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide
title_full_unstemmed Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide
title_short Rheological Behavior, Textural Properties, and Antioxidant Activity of <i>Porphyra yezoensis</i> Polysaccharide
title_sort rheological behavior textural properties and antioxidant activity of i porphyra yezoensis i polysaccharide
topic <i>Porphyra yezoensis</i> polysaccharide
rheological properties
gelation mechanism
calcium ions
antioxidant activities
url https://www.mdpi.com/1420-3049/30/4/882
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