Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway

Abstract As an enticing bone anabolic target, short‐term inhibition of Schnurri‐3 (SHN3) resulted in high‐bone mass due to augmented osteoblast activity. However, no studies are conducted to identify natural products targeting SHN3 inhibition. Herein, a screening strategy for the discovery of marine...

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Main Authors: Chun‐Lan Xie, Shang‐Hui Ye, Yu‐Ting Yue, Bao‐Hong Shi, Jing‐Ping Xu, Lian‐Jie Li, Zheng‐Biao Zou, Matthew B. Greenblatt, Na Li, Xian‐Wen Yang, Ren Xu
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202416140
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author Chun‐Lan Xie
Shang‐Hui Ye
Yu‐Ting Yue
Bao‐Hong Shi
Jing‐Ping Xu
Lian‐Jie Li
Zheng‐Biao Zou
Matthew B. Greenblatt
Na Li
Xian‐Wen Yang
Ren Xu
author_facet Chun‐Lan Xie
Shang‐Hui Ye
Yu‐Ting Yue
Bao‐Hong Shi
Jing‐Ping Xu
Lian‐Jie Li
Zheng‐Biao Zou
Matthew B. Greenblatt
Na Li
Xian‐Wen Yang
Ren Xu
author_sort Chun‐Lan Xie
collection DOAJ
description Abstract As an enticing bone anabolic target, short‐term inhibition of Schnurri‐3 (SHN3) resulted in high‐bone mass due to augmented osteoblast activity. However, no studies are conducted to identify natural products targeting SHN3 inhibition. Herein, a screening strategy for the discovery of marine compounds that facilitate osteoblast differentiation by targeting SHN3 silencing is presented. One leading quinolinone alkaloid, viridicatol (VDC), isolated from deep‐sea‐derived fungus, vigorously promotes osteogenic differentiation via the Wnt/SHN3 signaling pathway in osteoblasts, thereby preventing osteoporosis while enhancing bone‐fracture healing in a mouse model. Subsequently, the SDSSD (Ser, Asp, Ser, Ser, Asp) is further employed to engineer bone‐targeting nanovesicles (BT‐NVs) for the optimal delivery of VDC to osteoblasts, which mitigates the bone loss observed in a severe osteogenesis imperfecta model. Hence, these results initially uncover a promising marine natural product, VDC, targeting the Wnt/SHN3 pathway for the treatment of bone loss and highlighting its translational potential in clinical applications.
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spelling doaj-art-b245bbc9e96a4fe0b04031f36dfab4cb2025-08-20T02:32:26ZengWileyAdvanced Science2198-38442025-06-011221n/an/a10.1002/advs.202416140Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 PathwayChun‐Lan Xie0Shang‐Hui Ye1Yu‐Ting Yue2Bao‐Hong Shi3Jing‐Ping Xu4Lian‐Jie Li5Zheng‐Biao Zou6Matthew B. Greenblatt7Na Li8Xian‐Wen Yang9Ren Xu10Key Laboratory of Tropical Translational Medicine of Ministry of Education School of Basic Medicine and Life Sciences Hainan Academy of Medical Sciences Hainan Medical University No. 3 Xueyuan Road Haikou Hainan 571199 ChinaFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education School of Basic Medicine and Life Sciences Hainan Academy of Medical Sciences Hainan Medical University No. 3 Xueyuan Road Haikou Hainan 571199 ChinaDepartment of Pathology and Laboratory Medicine Weill Cornell Medicine New York NY 10065 USAFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education School of Basic Medicine and Life Sciences Hainan Academy of Medical Sciences Hainan Medical University No. 3 Xueyuan Road Haikou Hainan 571199 ChinaFujian Provincial Key Laboratory of Organ and Tissue Regeneration School of Medicine Xiamen University Xiamen 361102 ChinaAbstract As an enticing bone anabolic target, short‐term inhibition of Schnurri‐3 (SHN3) resulted in high‐bone mass due to augmented osteoblast activity. However, no studies are conducted to identify natural products targeting SHN3 inhibition. Herein, a screening strategy for the discovery of marine compounds that facilitate osteoblast differentiation by targeting SHN3 silencing is presented. One leading quinolinone alkaloid, viridicatol (VDC), isolated from deep‐sea‐derived fungus, vigorously promotes osteogenic differentiation via the Wnt/SHN3 signaling pathway in osteoblasts, thereby preventing osteoporosis while enhancing bone‐fracture healing in a mouse model. Subsequently, the SDSSD (Ser, Asp, Ser, Ser, Asp) is further employed to engineer bone‐targeting nanovesicles (BT‐NVs) for the optimal delivery of VDC to osteoblasts, which mitigates the bone loss observed in a severe osteogenesis imperfecta model. Hence, these results initially uncover a promising marine natural product, VDC, targeting the Wnt/SHN3 pathway for the treatment of bone loss and highlighting its translational potential in clinical applications.https://doi.org/10.1002/advs.202416140bone lossbone‐targeting nanovesiclesosteoblastsSchnurri‐3viridicatol
spellingShingle Chun‐Lan Xie
Shang‐Hui Ye
Yu‐Ting Yue
Bao‐Hong Shi
Jing‐Ping Xu
Lian‐Jie Li
Zheng‐Biao Zou
Matthew B. Greenblatt
Na Li
Xian‐Wen Yang
Ren Xu
Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway
Advanced Science
bone loss
bone‐targeting nanovesicles
osteoblasts
Schnurri‐3
viridicatol
title Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway
title_full Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway
title_fullStr Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway
title_full_unstemmed Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway
title_short Viridicatol from the Deep‐Sea‐Derived Fungus Alleviates Bone Loss by Targeting the Wnt/SHN3 Pathway
title_sort viridicatol from the deep sea derived fungus alleviates bone loss by targeting the wnt shn3 pathway
topic bone loss
bone‐targeting nanovesicles
osteoblasts
Schnurri‐3
viridicatol
url https://doi.org/10.1002/advs.202416140
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