Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway
ABSTRACT Background As one of the most common malignancies, cervical cancer (CC) seriously affects women's health. This study aimed to investigate the biological function of Serinc2 in CC. Methods Serinc2 expression was surveyed utilizing immunohistochemistry, western blot, and qRT‐PCR. CC cell...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-10-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.70296 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850132514649145344 |
|---|---|
| author | Xiaoping Wang Chen Jiang Qing Li |
| author_facet | Xiaoping Wang Chen Jiang Qing Li |
| author_sort | Xiaoping Wang |
| collection | DOAJ |
| description | ABSTRACT Background As one of the most common malignancies, cervical cancer (CC) seriously affects women's health. This study aimed to investigate the biological function of Serinc2 in CC. Methods Serinc2 expression was surveyed utilizing immunohistochemistry, western blot, and qRT‐PCR. CC cell viability, invasion, proliferation, migration, and apoptosis, were detected via CCK‐8, Transwell assay, colony formation, wound healing assay, and flow cytometry. Glucose consumption, lactate production, and ATP levels were determined by the corresponding kit. The protein expression of c‐Myc, PDK1, HK2, PFKP, LDHA, Snail, Vimentin, N‐cadherin, and E‐cadherin was detected via western blot. The interaction between the promoter of PFKP and Myc was confirmed through luciferase reporter assay and Chip assay. In vivo, to evaluate the function of Serinc2 on tumor growth, a xenograft mouse model was used. Results In CC tissues and cells, Serinc2 was upregulated. In CC cells, knockdown of Serinc2 suppressed cell invasion, proliferation, migration, decreased the expression of Snail, Vimentin, N‐cadherin, HK2, PFKP, LDHA, and PDK1, increased E‐cadherin expression, reduced glucose consumption and the production of lactate and ATP, and induced cell apoptosis; Serinc2 overexpression led to the opposite results. Mechanically, Serinc2 promoted Myc expression, and Myc induced PFKP expression. Furthermore, overexpressed Myc abolished the inhibitive influences of Serinc2 knockdown on the malignant behaviors of CC cells. Additionally, knockdown of Serinc2 inhibited tumor growth and reduced the protein expression of c‐Myc, PFKP, LDHA, and PDK1 in vivo. Conclusions Knockdown of Serinc2 inhibited the malignant progression of CC, which was achieved via Myc pathway. Our study provides novel insight into CC pathogenesis. |
| format | Article |
| id | doaj-art-b227a7269ba54e0580cef74ff5eec79e |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-b227a7269ba54e0580cef74ff5eec79e2025-08-20T02:32:11ZengWileyCancer Medicine2045-76342024-10-011319n/an/a10.1002/cam4.70296Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc PathwayXiaoping Wang0Chen Jiang1Qing Li2Department of Obstetrics and Gynecology Jinan Maternity and Child Care Hospital Jinan Shandong People's Republic of ChinaDepartment of Obstetrics and Gynecology Jinan Maternity and Child Care Hospital Jinan Shandong People's Republic of ChinaDepartment of Obstetrics and Gynecology Jinan Maternity and Child Care Hospital Jinan Shandong People's Republic of ChinaABSTRACT Background As one of the most common malignancies, cervical cancer (CC) seriously affects women's health. This study aimed to investigate the biological function of Serinc2 in CC. Methods Serinc2 expression was surveyed utilizing immunohistochemistry, western blot, and qRT‐PCR. CC cell viability, invasion, proliferation, migration, and apoptosis, were detected via CCK‐8, Transwell assay, colony formation, wound healing assay, and flow cytometry. Glucose consumption, lactate production, and ATP levels were determined by the corresponding kit. The protein expression of c‐Myc, PDK1, HK2, PFKP, LDHA, Snail, Vimentin, N‐cadherin, and E‐cadherin was detected via western blot. The interaction between the promoter of PFKP and Myc was confirmed through luciferase reporter assay and Chip assay. In vivo, to evaluate the function of Serinc2 on tumor growth, a xenograft mouse model was used. Results In CC tissues and cells, Serinc2 was upregulated. In CC cells, knockdown of Serinc2 suppressed cell invasion, proliferation, migration, decreased the expression of Snail, Vimentin, N‐cadherin, HK2, PFKP, LDHA, and PDK1, increased E‐cadherin expression, reduced glucose consumption and the production of lactate and ATP, and induced cell apoptosis; Serinc2 overexpression led to the opposite results. Mechanically, Serinc2 promoted Myc expression, and Myc induced PFKP expression. Furthermore, overexpressed Myc abolished the inhibitive influences of Serinc2 knockdown on the malignant behaviors of CC cells. Additionally, knockdown of Serinc2 inhibited tumor growth and reduced the protein expression of c‐Myc, PFKP, LDHA, and PDK1 in vivo. Conclusions Knockdown of Serinc2 inhibited the malignant progression of CC, which was achieved via Myc pathway. Our study provides novel insight into CC pathogenesis.https://doi.org/10.1002/cam4.70296cell proliferationcervical cancerglycolysisMycSerinc2 |
| spellingShingle | Xiaoping Wang Chen Jiang Qing Li Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway Cancer Medicine cell proliferation cervical cancer glycolysis Myc Serinc2 |
| title | Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway |
| title_full | Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway |
| title_fullStr | Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway |
| title_full_unstemmed | Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway |
| title_short | Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway |
| title_sort | serinc2 drives the progression of cervical cancer through regulating myc pathway |
| topic | cell proliferation cervical cancer glycolysis Myc Serinc2 |
| url | https://doi.org/10.1002/cam4.70296 |
| work_keys_str_mv | AT xiaopingwang serinc2drivestheprogressionofcervicalcancerthroughregulatingmycpathway AT chenjiang serinc2drivestheprogressionofcervicalcancerthroughregulatingmycpathway AT qingli serinc2drivestheprogressionofcervicalcancerthroughregulatingmycpathway |