Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance

Accurate and non-destructive monitoring of leaf chlorophyll content (LCC) is essential for assessing crop photosynthetic activity and nitrogen status in precision agriculture. This study introduces a phenology-aware machine learning framework that combines hyperspectral reflectance data with various...

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Main Authors: Chunbo Jiang, Yi Cheng, Yongfu Li, Lei Peng, Gangshang Dong, Ning Lai, Qinglong Geng
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Remote Sensing
Subjects:
Online Access:https://www.mdpi.com/2072-4292/17/15/2713
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author Chunbo Jiang
Yi Cheng
Yongfu Li
Lei Peng
Gangshang Dong
Ning Lai
Qinglong Geng
author_facet Chunbo Jiang
Yi Cheng
Yongfu Li
Lei Peng
Gangshang Dong
Ning Lai
Qinglong Geng
author_sort Chunbo Jiang
collection DOAJ
description Accurate and non-destructive monitoring of leaf chlorophyll content (LCC) is essential for assessing crop photosynthetic activity and nitrogen status in precision agriculture. This study introduces a phenology-aware machine learning framework that combines hyperspectral reflectance data with various regression models to estimate leaf chlorophyll content (LCC) in cotton at six key reproductive stages. Field experiments utilized synchronized spectral and SPAD measurements, incorporating spectral transformations—such as vegetation indices (VIs), first-order derivatives, and trilateration edge parameters (TEPs, a new set of geometric metrics for red-edge characterization)—for evaluation. Five regression approaches were evaluated, including univariate and multivariate linear models, along with three machine learning algorithms: Random Forest, K-Nearest Neighbor, and Support Vector Regression. Random Forest consistently outperformed the other models, achieving the highest R<sup>2</sup> (0.85) and the lowest RMSE (4.1) during the bud stage. Notably, the optimal prediction accuracy was achieved with fewer than five spectral features. The proposed framework demonstrates the potential for scalable, stage-specific monitoring of chlorophyll dynamics and offers valuable insights for large-scale crop management applications.
format Article
id doaj-art-b21c0a1d901c4592af034a178d5f2ec1
institution Kabale University
issn 2072-4292
language English
publishDate 2025-08-01
publisher MDPI AG
record_format Article
series Remote Sensing
spelling doaj-art-b21c0a1d901c4592af034a178d5f2ec12025-08-20T04:00:55ZengMDPI AGRemote Sensing2072-42922025-08-011715271310.3390/rs17152713Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral ReflectanceChunbo Jiang0Yi Cheng1Yongfu Li2Lei Peng3Gangshang Dong4Ning Lai5Qinglong Geng6Agricultural Engineering and Information Technology, College of Resources and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaAgricultural Engineering and Information Technology, College of Resources and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaXinjiang Academy of Agricultural Sciences, Resource and Environmental Information Technology Innovation Team, Urumqi 830091, ChinaXinjiang Academy of Agricultural Sciences, Resource and Environmental Information Technology Innovation Team, Urumqi 830091, ChinaXinjiang Academy of Agricultural Sciences, Resource and Environmental Information Technology Innovation Team, Urumqi 830091, ChinaXinjiang Academy of Agricultural Sciences, Resource and Environmental Information Technology Innovation Team, Urumqi 830091, ChinaXinjiang Academy of Agricultural Sciences, Resource and Environmental Information Technology Innovation Team, Urumqi 830091, ChinaAccurate and non-destructive monitoring of leaf chlorophyll content (LCC) is essential for assessing crop photosynthetic activity and nitrogen status in precision agriculture. This study introduces a phenology-aware machine learning framework that combines hyperspectral reflectance data with various regression models to estimate leaf chlorophyll content (LCC) in cotton at six key reproductive stages. Field experiments utilized synchronized spectral and SPAD measurements, incorporating spectral transformations—such as vegetation indices (VIs), first-order derivatives, and trilateration edge parameters (TEPs, a new set of geometric metrics for red-edge characterization)—for evaluation. Five regression approaches were evaluated, including univariate and multivariate linear models, along with three machine learning algorithms: Random Forest, K-Nearest Neighbor, and Support Vector Regression. Random Forest consistently outperformed the other models, achieving the highest R<sup>2</sup> (0.85) and the lowest RMSE (4.1) during the bud stage. Notably, the optimal prediction accuracy was achieved with fewer than five spectral features. The proposed framework demonstrates the potential for scalable, stage-specific monitoring of chlorophyll dynamics and offers valuable insights for large-scale crop management applications.https://www.mdpi.com/2072-4292/17/15/2713chlorophyll estimationhyperspectral reflectancerandom forest regressionphenological stagesvegetation indicescotton
spellingShingle Chunbo Jiang
Yi Cheng
Yongfu Li
Lei Peng
Gangshang Dong
Ning Lai
Qinglong Geng
Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance
Remote Sensing
chlorophyll estimation
hyperspectral reflectance
random forest regression
phenological stages
vegetation indices
cotton
title Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance
title_full Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance
title_fullStr Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance
title_full_unstemmed Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance
title_short Phenology-Aware Machine Learning Framework for Chlorophyll Estimation in Cotton Using Hyperspectral Reflectance
title_sort phenology aware machine learning framework for chlorophyll estimation in cotton using hyperspectral reflectance
topic chlorophyll estimation
hyperspectral reflectance
random forest regression
phenological stages
vegetation indices
cotton
url https://www.mdpi.com/2072-4292/17/15/2713
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AT leipeng phenologyawaremachinelearningframeworkforchlorophyllestimationincottonusinghyperspectralreflectance
AT gangshangdong phenologyawaremachinelearningframeworkforchlorophyllestimationincottonusinghyperspectralreflectance
AT ninglai phenologyawaremachinelearningframeworkforchlorophyllestimationincottonusinghyperspectralreflectance
AT qinglonggeng phenologyawaremachinelearningframeworkforchlorophyllestimationincottonusinghyperspectralreflectance