Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798
<i>Bifidobacterium</i> is among the dominant flora in the healthy intestine of the human body. It has many probiotic effects such as lowering cholesterol, inhibiting tumors, and regulating immunity. However, fluctuations in culture conditions during cultivation will lead to a decrease in...
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MDPI AG
2024-10-01
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| Series: | Fermentation |
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| author | Kailong Liu Yiting Liu Zhan Yang Jie Yu Guoqiang Yao |
| author_facet | Kailong Liu Yiting Liu Zhan Yang Jie Yu Guoqiang Yao |
| author_sort | Kailong Liu |
| collection | DOAJ |
| description | <i>Bifidobacterium</i> is among the dominant flora in the healthy intestine of the human body. It has many probiotic effects such as lowering cholesterol, inhibiting tumors, and regulating immunity. However, fluctuations in culture conditions during cultivation will lead to a decrease in the number of active bacteria. Therefore, more precise control of culture conditions is required to reduce the activity damage caused by environmental fluctuations. Based on this, this study utilized a fully automatic intelligent fermentation tank to develop a cultivation technique suitable for improving the activity and biomass of <i>Bifidobacterium breve</i> B2798. The results show that, under a cultivation temperature of 38.0 °C, the highest viable cell count, which is (2.56 ± 0.04) × 10<sup>10</sup> CFU/mL, can be achieved in the culture medium, with the conclusion that the fermentation endpoint should be controlled at the end period of bacteria logarithmic growth when there is the highest viable cell count and bacterial activity in the culture medium. This study has elucidated the influences of different temperatures on the biomass, viable cell count, and activity of <i>Bifidobacterium breve</i> B2798, providing basic data for the later development of industrialized processing techniques for this bacteria strain. |
| format | Article |
| id | doaj-art-b21459df4d144700a9dc7d31d48a6431 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-b21459df4d144700a9dc7d31d48a64312025-08-20T02:28:14ZengMDPI AGFermentation2311-56372024-10-01101155310.3390/fermentation10110553Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798Kailong Liu0Yiting Liu1Zhan Yang2Jie Yu3Guoqiang Yao4Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China<i>Bifidobacterium</i> is among the dominant flora in the healthy intestine of the human body. It has many probiotic effects such as lowering cholesterol, inhibiting tumors, and regulating immunity. However, fluctuations in culture conditions during cultivation will lead to a decrease in the number of active bacteria. Therefore, more precise control of culture conditions is required to reduce the activity damage caused by environmental fluctuations. Based on this, this study utilized a fully automatic intelligent fermentation tank to develop a cultivation technique suitable for improving the activity and biomass of <i>Bifidobacterium breve</i> B2798. The results show that, under a cultivation temperature of 38.0 °C, the highest viable cell count, which is (2.56 ± 0.04) × 10<sup>10</sup> CFU/mL, can be achieved in the culture medium, with the conclusion that the fermentation endpoint should be controlled at the end period of bacteria logarithmic growth when there is the highest viable cell count and bacterial activity in the culture medium. This study has elucidated the influences of different temperatures on the biomass, viable cell count, and activity of <i>Bifidobacterium breve</i> B2798, providing basic data for the later development of industrialized processing techniques for this bacteria strain.https://www.mdpi.com/2311-5637/10/11/553<i>Bifidobacterium breve</i> B2798culture temperaturebiomassbacterial activity |
| spellingShingle | Kailong Liu Yiting Liu Zhan Yang Jie Yu Guoqiang Yao Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798 Fermentation <i>Bifidobacterium breve</i> B2798 culture temperature biomass bacterial activity |
| title | Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798 |
| title_full | Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798 |
| title_fullStr | Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798 |
| title_full_unstemmed | Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798 |
| title_short | Regulation of Cultivation Temperature on Biomass and Activity of <i>Bifidobacterium breve</i> B2798 |
| title_sort | regulation of cultivation temperature on biomass and activity of i bifidobacterium breve i b2798 |
| topic | <i>Bifidobacterium breve</i> B2798 culture temperature biomass bacterial activity |
| url | https://www.mdpi.com/2311-5637/10/11/553 |
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