Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity
Objective: By inhibiting the synthesis of thymidine and purine, and thereby DNA synthesis, Methotrexate (MTX), suppresses the proliferation of cancer cells. It is thought that the side-effect mechanism is related to oxidant molecules derived from MTX metabolism. In this study, we examined whether th...
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Istanbul University Press
2021-12-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/1A6B6E4E39224CADBEFDAB48A3DBD25D |
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| author | Büşra Ertaş Feyza Berin Turan Dilek Özbeyli Refiye Yanardağ Özlem Saçan Göksel Şener |
| author_facet | Büşra Ertaş Feyza Berin Turan Dilek Özbeyli Refiye Yanardağ Özlem Saçan Göksel Şener |
| author_sort | Büşra Ertaş |
| collection | DOAJ |
| description | Objective: By inhibiting the synthesis of thymidine and purine, and thereby DNA synthesis, Methotrexate (MTX), suppresses the proliferation of cancer cells. It is thought that the side-effect mechanism is related to oxidant molecules derived from MTX metabolism. In this study, we examined whether the Petroselinum crispum extracts (PCr; parsley) of which the antioxidant properties have been previously shown, was protective against MTX induced liver damage. Materials and Methods: Sprague Dawley rats (female/male; 200-250 g) were used. MTX was injected intraperitoneally and PCr extract was given orally. A single dose of 20mg/kg MTX was administered to the groups that were to experience hepatotoxicity. Then, a physiological saline (MTX group) or PCr (2 g/kg, MTX + PCr group) treatment was applied for 5 days. The same treatments were applied to the other groups (control group, PCr group) for 5 days after a single dose saline injection. At the end of the study, the biochemical parameters were examined in the blood and liver tissues taken from animals sacrificed by decapitation. Results: MTX caused a significant increase in malondialdehyde and collagen levels and myeloperoxidase and caspase-3 activities, while glutathione levels were found to have decreased. PCr treatment showed protective efficacy by preventing these increases. Conclusion: It appears that the administration of PCr to MTX treated rats prevented the accumulation of lipid peroxides, inflamatory reactions and depletion of antioxidant glutathione, and thus protected liver tissues against oxidative stress. |
| format | Article |
| id | doaj-art-b20ded80c72c478f9057e5d67bd3733e |
| institution | OA Journals |
| issn | 2618-6144 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-b20ded80c72c478f9057e5d67bd3733e2025-08-20T02:10:56ZengIstanbul University PressEuropean Journal of Biology2618-61442021-12-0180217317810.26650/EurJBiol.2021.1023136123456Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced HepatotoxicityBüşra Ertaş0https://orcid.org/0000-0001-8374-1098Feyza Berin Turan1https://orcid.org/0000-0002-0436-7876Dilek Özbeyli2https://orcid.org/0000-0002-4141-6913Refiye Yanardağ3https://orcid.org/0000-0003-4185-4363Özlem Saçan4https://orcid.org/0000-0001-6503-4613Göksel Şener5https://orcid.org/0000-0001-7444-6193Marmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeFenerbahçe Üniversitesi, Istanbul, TurkiyeObjective: By inhibiting the synthesis of thymidine and purine, and thereby DNA synthesis, Methotrexate (MTX), suppresses the proliferation of cancer cells. It is thought that the side-effect mechanism is related to oxidant molecules derived from MTX metabolism. In this study, we examined whether the Petroselinum crispum extracts (PCr; parsley) of which the antioxidant properties have been previously shown, was protective against MTX induced liver damage. Materials and Methods: Sprague Dawley rats (female/male; 200-250 g) were used. MTX was injected intraperitoneally and PCr extract was given orally. A single dose of 20mg/kg MTX was administered to the groups that were to experience hepatotoxicity. Then, a physiological saline (MTX group) or PCr (2 g/kg, MTX + PCr group) treatment was applied for 5 days. The same treatments were applied to the other groups (control group, PCr group) for 5 days after a single dose saline injection. At the end of the study, the biochemical parameters were examined in the blood and liver tissues taken from animals sacrificed by decapitation. Results: MTX caused a significant increase in malondialdehyde and collagen levels and myeloperoxidase and caspase-3 activities, while glutathione levels were found to have decreased. PCr treatment showed protective efficacy by preventing these increases. Conclusion: It appears that the administration of PCr to MTX treated rats prevented the accumulation of lipid peroxides, inflamatory reactions and depletion of antioxidant glutathione, and thus protected liver tissues against oxidative stress.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/1A6B6E4E39224CADBEFDAB48A3DBD25Dmethotrexatepetroselinum crispumhepatotoxicityoxidative injuryanti-inflammatory |
| spellingShingle | Büşra Ertaş Feyza Berin Turan Dilek Özbeyli Refiye Yanardağ Özlem Saçan Göksel Şener Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity European Journal of Biology methotrexate petroselinum crispum hepatotoxicity oxidative injury anti-inflammatory |
| title | Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity |
| title_full | Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity |
| title_fullStr | Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity |
| title_full_unstemmed | Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity |
| title_short | Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity |
| title_sort | protective effects of petroselinum crispum parsley extract against methotrexate induced hepatotoxicity |
| topic | methotrexate petroselinum crispum hepatotoxicity oxidative injury anti-inflammatory |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/1A6B6E4E39224CADBEFDAB48A3DBD25D |
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