Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations
A simple extractive spectrophotometric technique has been developed and validated for the determination of miconazole nitrate in pure and pharmaceutical formulations. The method is based on the formation of a chloroform-soluble ion-pair complex between the drug and bromocresol green (BCG) dye in an...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/2191072 |
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| author | Tadele Eticha Getu Kahsay Teklebrhan Hailu Tesfamichael Gebretsadikan Fitsum Asefa Hailekiros Gebretsadik Boovizhikannan Thangabalan |
| author_facet | Tadele Eticha Getu Kahsay Teklebrhan Hailu Tesfamichael Gebretsadikan Fitsum Asefa Hailekiros Gebretsadik Boovizhikannan Thangabalan |
| author_sort | Tadele Eticha |
| collection | DOAJ |
| description | A simple extractive spectrophotometric technique has been developed and validated for the determination of miconazole nitrate in pure and pharmaceutical formulations. The method is based on the formation of a chloroform-soluble ion-pair complex between the drug and bromocresol green (BCG) dye in an acidic medium. The complex showed absorption maxima at 422 nm, and the system obeys Beer’s law in the concentration range of 1–30 µg/mL with molar absorptivity of 2.285 × 104 L/mol/cm. The composition of the complex was studied by Job’s method of continuous variation, and the results revealed that the mole ratio of drug : BCG is 1 : 1. Full factorial design was used to optimize the effect of variable factors, and the method was validated based on the ICH guidelines. The method was applied for the determination of miconazole nitrate in real samples. |
| format | Article |
| id | doaj-art-b208b7e4fcae4c0ba54cc4b8f30e7def |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-b208b7e4fcae4c0ba54cc4b8f30e7def2025-02-03T01:11:43ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/21910722191072Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical FormulationsTadele Eticha0Getu Kahsay1Teklebrhan Hailu2Tesfamichael Gebretsadikan3Fitsum Asefa4Hailekiros Gebretsadik5Boovizhikannan Thangabalan6School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaA simple extractive spectrophotometric technique has been developed and validated for the determination of miconazole nitrate in pure and pharmaceutical formulations. The method is based on the formation of a chloroform-soluble ion-pair complex between the drug and bromocresol green (BCG) dye in an acidic medium. The complex showed absorption maxima at 422 nm, and the system obeys Beer’s law in the concentration range of 1–30 µg/mL with molar absorptivity of 2.285 × 104 L/mol/cm. The composition of the complex was studied by Job’s method of continuous variation, and the results revealed that the mole ratio of drug : BCG is 1 : 1. Full factorial design was used to optimize the effect of variable factors, and the method was validated based on the ICH guidelines. The method was applied for the determination of miconazole nitrate in real samples.http://dx.doi.org/10.1155/2018/2191072 |
| spellingShingle | Tadele Eticha Getu Kahsay Teklebrhan Hailu Tesfamichael Gebretsadikan Fitsum Asefa Hailekiros Gebretsadik Boovizhikannan Thangabalan Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations Journal of Analytical Methods in Chemistry |
| title | Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations |
| title_full | Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations |
| title_fullStr | Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations |
| title_full_unstemmed | Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations |
| title_short | Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations |
| title_sort | development and validation of an extractive spectrophotometric method for miconazole nitrate assay in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2018/2191072 |
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