Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum
<b>Background/Objectives</b>: Degenerative processes of the hip joint increasingly affect not only the articular cartilage but also periarticular structures such as the joint capsule and acetabular labrum. This study aimed to investigate the structural and molecular changes occurring in...
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2025-07-01
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| author | Riana Maria Huzum Bogdan Huzum Marius Valeriu Hînganu Ludmila Lozneanu Fabian Cezar Lupu Delia Hînganu |
| author_facet | Riana Maria Huzum Bogdan Huzum Marius Valeriu Hînganu Ludmila Lozneanu Fabian Cezar Lupu Delia Hînganu |
| author_sort | Riana Maria Huzum |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Degenerative processes of the hip joint increasingly affect not only the articular cartilage but also periarticular structures such as the joint capsule and acetabular labrum. This study aimed to investigate the structural and molecular changes occurring in these tissues during advanced hip osteoarthritis. <b>Methods</b>: A combined analysis using immunohistochemistry (IHC), scanning electron microscopy (SEM), and micro-computed tomography (microCT) was conducted on tissue samples from patients undergoing total hip arthroplasty and from controls with morphologically normal joints. Markers associated with proliferation (Ki67), inflammation (CD68), angiogenesis (CD31, ERG), chondrogenesis (SOX9), and lubrication (Lubricin) were evaluated. <b>Results</b>: The pathological group showed increased expression of Ki67, CD68, CD31, ERG, and SOX9, with a notable decrease in Lubricin. SEM analysis revealed ultrastructural disorganization, collagen fragmentation, and neovascular remodeling in degenerative samples. A significant correlation between structural damage and molecular expression was identified. <b>Conclusions</b>: These results suggest that joint capsule and acetabular labrum degeneration are interconnected and reflect a broader pathophysiological continuum, supporting the use of integrated IHC and SEM profiling for early detection and targeted intervention in hip joint disease. |
| format | Article |
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| institution | Kabale University |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Diagnostics |
| spelling | doaj-art-b207e2ebe4b049e687e24950c9b1c7e22025-08-20T03:35:58ZengMDPI AGDiagnostics2075-44182025-07-011515193210.3390/diagnostics15151932Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular LabrumRiana Maria Huzum0Bogdan Huzum1Marius Valeriu Hînganu2Ludmila Lozneanu3Fabian Cezar Lupu4Delia Hînganu5Department of Radiology, Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 400347 Iasi, RomaniaDepartment of Orthopedics and Traumatology, Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 400347 Iasi, RomaniaDepartment of Morpho-Functional Sciences I, Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Morpho-Functional Sciences I, Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Mechanical, Mechatronics and Robotics Engineering, Mechanical Engineering Faculty, “Gheorghe Asachi” Technical University of Iasi, 700050 Iasi, RomaniaDepartment of Morpho-Functional Sciences I, Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania<b>Background/Objectives</b>: Degenerative processes of the hip joint increasingly affect not only the articular cartilage but also periarticular structures such as the joint capsule and acetabular labrum. This study aimed to investigate the structural and molecular changes occurring in these tissues during advanced hip osteoarthritis. <b>Methods</b>: A combined analysis using immunohistochemistry (IHC), scanning electron microscopy (SEM), and micro-computed tomography (microCT) was conducted on tissue samples from patients undergoing total hip arthroplasty and from controls with morphologically normal joints. Markers associated with proliferation (Ki67), inflammation (CD68), angiogenesis (CD31, ERG), chondrogenesis (SOX9), and lubrication (Lubricin) were evaluated. <b>Results</b>: The pathological group showed increased expression of Ki67, CD68, CD31, ERG, and SOX9, with a notable decrease in Lubricin. SEM analysis revealed ultrastructural disorganization, collagen fragmentation, and neovascular remodeling in degenerative samples. A significant correlation between structural damage and molecular expression was identified. <b>Conclusions</b>: These results suggest that joint capsule and acetabular labrum degeneration are interconnected and reflect a broader pathophysiological continuum, supporting the use of integrated IHC and SEM profiling for early detection and targeted intervention in hip joint disease.https://www.mdpi.com/2075-4418/15/15/1932hip capsuleacetabular labrumosteoarthritisimmunohistochemistrySEMSOX9 |
| spellingShingle | Riana Maria Huzum Bogdan Huzum Marius Valeriu Hînganu Ludmila Lozneanu Fabian Cezar Lupu Delia Hînganu Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum Diagnostics hip capsule acetabular labrum osteoarthritis immunohistochemistry SEM SOX9 |
| title | Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum |
| title_full | Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum |
| title_fullStr | Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum |
| title_full_unstemmed | Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum |
| title_short | Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum |
| title_sort | immunohistochemical and ultrastructural study of the degenerative processes of the hip joint capsule and acetabular labrum |
| topic | hip capsule acetabular labrum osteoarthritis immunohistochemistry SEM SOX9 |
| url | https://www.mdpi.com/2075-4418/15/15/1932 |
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