Viral loads in throat and anal swabs in children infected with SARS-CoV-2
Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performanc...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1771219 |
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| author | Chunhui Yuan Hongmin Zhu Yuan Yang Xiaonan Cai Feiyan Xiang Huan Wu Cong Yao Yun Xiang Han Xiao |
| author_facet | Chunhui Yuan Hongmin Zhu Yuan Yang Xiaonan Cai Feiyan Xiang Huan Wu Cong Yao Yun Xiang Han Xiao |
| author_sort | Chunhui Yuan |
| collection | DOAJ |
| description | Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children’s Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients. |
| format | Article |
| id | doaj-art-b200c909c45c45ef8d0680c8d899007e |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-b200c909c45c45ef8d0680c8d899007e2025-08-20T03:08:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911233123710.1080/22221751.2020.1771219Viral loads in throat and anal swabs in children infected with SARS-CoV-2Chunhui Yuan0Hongmin Zhu1Yuan Yang2Xiaonan Cai3Feiyan Xiang4Huan Wu5Cong Yao6Yun Xiang7Han Xiao8Department of Laboratory Medicine, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaDepartment of Neurology, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaInstitute of Maternal and Child Health, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaInstitute of Maternal and Child Health, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaInstitute of Maternal and Child Health, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaDepartment of Laboratory Medicine, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaHealth Care Department, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaDepartment of Laboratory Medicine, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaInstitute of Maternal and Child Health, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaReal-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children’s Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients.https://www.tandfonline.com/doi/10.1080/22221751.2020.1771219SARS-CoV-2paediatric patientsRT-PCRdiagnostic potentialviral load |
| spellingShingle | Chunhui Yuan Hongmin Zhu Yuan Yang Xiaonan Cai Feiyan Xiang Huan Wu Cong Yao Yun Xiang Han Xiao Viral loads in throat and anal swabs in children infected with SARS-CoV-2 Emerging Microbes and Infections SARS-CoV-2 paediatric patients RT-PCR diagnostic potential viral load |
| title | Viral loads in throat and anal swabs in children infected with SARS-CoV-2 |
| title_full | Viral loads in throat and anal swabs in children infected with SARS-CoV-2 |
| title_fullStr | Viral loads in throat and anal swabs in children infected with SARS-CoV-2 |
| title_full_unstemmed | Viral loads in throat and anal swabs in children infected with SARS-CoV-2 |
| title_short | Viral loads in throat and anal swabs in children infected with SARS-CoV-2 |
| title_sort | viral loads in throat and anal swabs in children infected with sars cov 2 |
| topic | SARS-CoV-2 paediatric patients RT-PCR diagnostic potential viral load |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1771219 |
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