Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower

Abstract The degradable film can solve the problem that the traditional plastic film is difficult to recycle and heavy pollution for a long time. The effects of degraded film mulching on microbial diversity are significant. However, the responses of relevant microbial communities to degraded film mu...

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Main Authors: Tiantian Meng, Hengtong Bu, Xiangqian Zhang, Xuanyi Chen, Weini Wang, Min Zhao, Junmei Liu, Jianwei Zhang, Dejian Zhang, Zhanyuan Lu, Xiaoyu Zhao
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-03213-2
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author Tiantian Meng
Hengtong Bu
Xiangqian Zhang
Xuanyi Chen
Weini Wang
Min Zhao
Junmei Liu
Jianwei Zhang
Dejian Zhang
Zhanyuan Lu
Xiaoyu Zhao
author_facet Tiantian Meng
Hengtong Bu
Xiangqian Zhang
Xuanyi Chen
Weini Wang
Min Zhao
Junmei Liu
Jianwei Zhang
Dejian Zhang
Zhanyuan Lu
Xiaoyu Zhao
author_sort Tiantian Meng
collection DOAJ
description Abstract The degradable film can solve the problem that the traditional plastic film is difficult to recycle and heavy pollution for a long time. The effects of degraded film mulching on microbial diversity are significant. However, the responses of relevant microbial communities to degraded film mulching in different ecological niches (e.g., bulk soil, rhizosphere and endosphere) of sunflower roots are poorly understood. This study analyzed the effects of plastics film mulching on bacterial and fungal α-diversities (Shannon index), community assembly process, key dominant species of sunflower different ecological niches in roots. The results showed that degradable film mulching significantly increased the α-diversity (Shannon index) of bulk soil and rhizosphere soil bacteria and decreased the α-diversity of fungi (Shannon index), and the mulching treatment promoted the gradual shift of the rhizosphere microbial community assembly process to a deterministic process. Degradation film mulching increased the connectivity and complexity of bacterial networks and decreased the complexity of fungal networks. Plastic film mulching improves soil nutrients, temperature and moisture, enhances the positive correlation among microorganisms. At the same time, core species such as Amycolatopsis, Rhizobiaceae, and Sphingomonas that recruit beneficial microorganisms and accelerate the degradation of plastic film are significantly enriched. Degradable film covering promoted soil nutrient cycling, increased urease, alkaline phosphatase, sucrase, and thus increased sunflower yield. A comprehensive analysis of random forest and structural equations showed that the main driving microbial factors of yield were bulk soil bacterial diversity and endosphere fungal diversity. This study provides new ideas for the analysis of soil microbial mutual feedback mechanisms between degraded film mulch and rhizosphere ecosystems.
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issn 2045-2322
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publishDate 2025-05-01
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spelling doaj-art-b1fce76a269d4ebdab61b762bbc21e0e2025-08-20T02:03:31ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-03213-2Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflowerTiantian Meng0Hengtong Bu1Xiangqian Zhang2Xuanyi Chen3Weini Wang4Min Zhao5Junmei Liu6Jianwei Zhang7Dejian Zhang8Zhanyuan Lu9Xiaoyu Zhao10Hebei Agricultural UniversityInner Mongolia UniversityInner Mongolia Academy of Agricultural and Animal Husbandry SciencesInner Mongolia UniversityErdos Agricultural and Animal Husbandry Technology Extension CenterInner Mongolia UniversityErdos Agricultural and Animal Husbandry Technology Extension CenterInner Mongolia Academy of Agricultural and Animal Husbandry SciencesInner Mongolia UniversityHebei Agricultural UniversityInner Mongolia Academy of Agricultural and Animal Husbandry SciencesAbstract The degradable film can solve the problem that the traditional plastic film is difficult to recycle and heavy pollution for a long time. The effects of degraded film mulching on microbial diversity are significant. However, the responses of relevant microbial communities to degraded film mulching in different ecological niches (e.g., bulk soil, rhizosphere and endosphere) of sunflower roots are poorly understood. This study analyzed the effects of plastics film mulching on bacterial and fungal α-diversities (Shannon index), community assembly process, key dominant species of sunflower different ecological niches in roots. The results showed that degradable film mulching significantly increased the α-diversity (Shannon index) of bulk soil and rhizosphere soil bacteria and decreased the α-diversity of fungi (Shannon index), and the mulching treatment promoted the gradual shift of the rhizosphere microbial community assembly process to a deterministic process. Degradation film mulching increased the connectivity and complexity of bacterial networks and decreased the complexity of fungal networks. Plastic film mulching improves soil nutrients, temperature and moisture, enhances the positive correlation among microorganisms. At the same time, core species such as Amycolatopsis, Rhizobiaceae, and Sphingomonas that recruit beneficial microorganisms and accelerate the degradation of plastic film are significantly enriched. Degradable film covering promoted soil nutrient cycling, increased urease, alkaline phosphatase, sucrase, and thus increased sunflower yield. A comprehensive analysis of random forest and structural equations showed that the main driving microbial factors of yield were bulk soil bacterial diversity and endosphere fungal diversity. This study provides new ideas for the analysis of soil microbial mutual feedback mechanisms between degraded film mulch and rhizosphere ecosystems.https://doi.org/10.1038/s41598-025-03213-2Degradation filmSunflowerRoot nicheMicrobial community assemblyYield
spellingShingle Tiantian Meng
Hengtong Bu
Xiangqian Zhang
Xuanyi Chen
Weini Wang
Min Zhao
Junmei Liu
Jianwei Zhang
Dejian Zhang
Zhanyuan Lu
Xiaoyu Zhao
Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
Scientific Reports
Degradation film
Sunflower
Root niche
Microbial community assembly
Yield
title Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
title_full Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
title_fullStr Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
title_full_unstemmed Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
title_short Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
title_sort degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower
topic Degradation film
Sunflower
Root niche
Microbial community assembly
Yield
url https://doi.org/10.1038/s41598-025-03213-2
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