Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry

At present, there is no screening test for the early detecting and monitoring of ovarian cancer, one of the most lethal form of gynaecological malig- nancy in the worldwide. In this study, the new methodology for the search of tumor markers of ovarian cancer, involving profiling the low-molec- ular...

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Main Authors: V. E. Shevchenko, N. E. Arnotskaya, E. V. Ogorodnikova, N. R. Pogosyan, A. N. Gritsay, K. I. Zhordania
Format: Article
Language:Russian
Published: ABV-press 2014-07-01
Series:Опухоли женской репродуктивной системы
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Online Access:https://ojrs.abvpress.ru/ojrs/article/view/64
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author V. E. Shevchenko
N. E. Arnotskaya
E. V. Ogorodnikova
N. R. Pogosyan
A. N. Gritsay
K. I. Zhordania
author_facet V. E. Shevchenko
N. E. Arnotskaya
E. V. Ogorodnikova
N. R. Pogosyan
A. N. Gritsay
K. I. Zhordania
author_sort V. E. Shevchenko
collection DOAJ
description At present, there is no screening test for the early detecting and monitoring of ovarian cancer, one of the most lethal form of gynaecological malig- nancy in the worldwide. In this study, the new methodology for the search of tumor markers of ovarian cancer, involving profiling the low-molec- ular blood plasma proteomes, is developed, unified and approved. The given approach included three basic components: pre-preparation of samples, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics software for mass spectral data process- ing. Opportunities and prospects of the developed approach for the detection of potential ovarian cancer markers were shown. For search of po- tential tumor markers, screening of 40 blood plasma samples from ovarian cancer patients and 48 control samples were carried out. As a result of the present research, peptides/polypeptides which can be used in future for detecting this pathology were found out.
format Article
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institution Kabale University
issn 1994-4098
1999-8627
language Russian
publishDate 2014-07-01
publisher ABV-press
record_format Article
series Опухоли женской репродуктивной системы
spelling doaj-art-b1ebfc600ffa4636a563d824284978d32025-08-20T04:00:02ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272014-07-0103-4909610.17650/1994-4098-2013-0-3-4-90-9679Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometryV. E. Shevchenko0N. E. Arnotskaya1E. V. Ogorodnikova2N. R. Pogosyan3A. N. Gritsay4K. I. Zhordania5Oncoproteomics Laboratory, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowOncoproteomics Laboratory, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowBlood Transfusion Department, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowGynecology Department, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowGynecology Department, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowGynecology Department, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowAt present, there is no screening test for the early detecting and monitoring of ovarian cancer, one of the most lethal form of gynaecological malig- nancy in the worldwide. In this study, the new methodology for the search of tumor markers of ovarian cancer, involving profiling the low-molec- ular blood plasma proteomes, is developed, unified and approved. The given approach included three basic components: pre-preparation of samples, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics software for mass spectral data process- ing. Opportunities and prospects of the developed approach for the detection of potential ovarian cancer markers were shown. For search of po- tential tumor markers, screening of 40 blood plasma samples from ovarian cancer patients and 48 control samples were carried out. As a result of the present research, peptides/polypeptides which can be used in future for detecting this pathology were found out.https://ojrs.abvpress.ru/ojrs/article/view/64biomarkersovarian cancerproteomeblood plasmamass spectrometry
spellingShingle V. E. Shevchenko
N. E. Arnotskaya
E. V. Ogorodnikova
N. R. Pogosyan
A. N. Gritsay
K. I. Zhordania
Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
Опухоли женской репродуктивной системы
biomarkers
ovarian cancer
proteome
blood plasma
mass spectrometry
title Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
title_full Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
title_fullStr Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
title_full_unstemmed Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
title_short Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
title_sort discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry
topic biomarkers
ovarian cancer
proteome
blood plasma
mass spectrometry
url https://ojrs.abvpress.ru/ojrs/article/view/64
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AT evogorodnikova discoveryofpotentialovariancancerbiomarkersusinglowmolecularweightbloodplasmaproteomeprofilingbymassspectrometry
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