Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese
Studies have demonstrated significant alterations in ovarian oxidative stress levels, ovarian degeneration, and follicular atresia during the broody period in geese. The results of this study showed that during the broody period, geese exhibited degraded ovarian tissues, disrupted follicular develop...
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2025-01-01
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| author | Ning Zhou Yaoyao Zhang Youluan Jiang Wang Gu Shuai Zhao Wanwipa Vongsangnak Yang Zhang Qi Xu Yu Zhang |
| author_facet | Ning Zhou Yaoyao Zhang Youluan Jiang Wang Gu Shuai Zhao Wanwipa Vongsangnak Yang Zhang Qi Xu Yu Zhang |
| author_sort | Ning Zhou |
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| description | Studies have demonstrated significant alterations in ovarian oxidative stress levels, ovarian degeneration, and follicular atresia during the broody period in geese. The results of this study showed that during the broody period, geese exhibited degraded ovarian tissues, disrupted follicular development, a thinner granulosa cell layer, and lower levels of ovarian hormones E2, P4, and AMH. Antioxidant activity (GSH, CAT, SOD, T-AOC, and the content of H<sub>2</sub>O<sub>2</sub>) and the mRNA expression levels of antioxidant genes (GPX, SOD-1, SOD-2, CAT, COX-2, and Hsp70) were significantly higher in pre-broody geese compared to laying geese, while the expression of apoptosis-related genes (p53, Caspase-3, and Caspase-9) increased and the anti-apoptotic gene Bcl-2 decreased. Additionally, proteomic analysis identified 703 differentially expressed proteins (DEPs), primarily concentrated in the GO categories of the biological process (biological regulation, response to stimulus, etc.) and enriched in the KEGG pathways (PI3K-Akt signaling pathway, etc.). Among them, XDH was central to the regulatory network. Furthermore, Western blotting revealed higher expression of XDH in the ovaries of pre-broody geese than those of laying geese. Pearson correlation analysis indicated a significant correlation between XDH expression and oxidative stress markers in the ovaries of geese (r > 0.75). Overall, these results demonstrated that geese experience ovarian atrophy and remarkably increased oxidative stress during the broody period, suggesting that XDH may be a key driver of broodiness in geese. |
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| spelling | doaj-art-b1dc721ffb104727a025b778eda58cc52025-01-24T13:17:57ZengMDPI AGAnimals2076-26152025-01-0115218210.3390/ani15020182Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of GeeseNing Zhou0Yaoyao Zhang1Youluan Jiang2Wang Gu3Shuai Zhao4Wanwipa Vongsangnak5Yang Zhang6Qi Xu7Yu Zhang8Jiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaDepartment of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, ChinaStudies have demonstrated significant alterations in ovarian oxidative stress levels, ovarian degeneration, and follicular atresia during the broody period in geese. The results of this study showed that during the broody period, geese exhibited degraded ovarian tissues, disrupted follicular development, a thinner granulosa cell layer, and lower levels of ovarian hormones E2, P4, and AMH. Antioxidant activity (GSH, CAT, SOD, T-AOC, and the content of H<sub>2</sub>O<sub>2</sub>) and the mRNA expression levels of antioxidant genes (GPX, SOD-1, SOD-2, CAT, COX-2, and Hsp70) were significantly higher in pre-broody geese compared to laying geese, while the expression of apoptosis-related genes (p53, Caspase-3, and Caspase-9) increased and the anti-apoptotic gene Bcl-2 decreased. Additionally, proteomic analysis identified 703 differentially expressed proteins (DEPs), primarily concentrated in the GO categories of the biological process (biological regulation, response to stimulus, etc.) and enriched in the KEGG pathways (PI3K-Akt signaling pathway, etc.). Among them, XDH was central to the regulatory network. Furthermore, Western blotting revealed higher expression of XDH in the ovaries of pre-broody geese than those of laying geese. Pearson correlation analysis indicated a significant correlation between XDH expression and oxidative stress markers in the ovaries of geese (r > 0.75). Overall, these results demonstrated that geese experience ovarian atrophy and remarkably increased oxidative stress during the broody period, suggesting that XDH may be a key driver of broodiness in geese.https://www.mdpi.com/2076-2615/15/2/182gooseXDHoxidative stressbroody behavior |
| spellingShingle | Ning Zhou Yaoyao Zhang Youluan Jiang Wang Gu Shuai Zhao Wanwipa Vongsangnak Yang Zhang Qi Xu Yu Zhang Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese Animals goose XDH oxidative stress broody behavior |
| title | Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese |
| title_full | Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese |
| title_fullStr | Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese |
| title_full_unstemmed | Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese |
| title_short | Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese |
| title_sort | quantitative proteomics analysis reveals xdh related with ovarian oxidative stress involved in broodiness of geese |
| topic | goose XDH oxidative stress broody behavior |
| url | https://www.mdpi.com/2076-2615/15/2/182 |
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