Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method
Background: The therapeutic properties of the ethanol extract derived from the stem bark of Rhizophora apiculata have been investigated. Nevertheless, there is a lack of studies regarding its acute toxicity. Objective: This research aims to evaluate the toxicity of ethanolic extract of R. apicul...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Society for Biochemistry and Molecular Biology
2024-07-01
|
| Series: | Acta Biochimica Indonesiana |
| Subjects: | |
| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/144 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825199598392573952 |
|---|---|
| author | Syazili Mustofa Ilma Puteri Hutami Dwi Sarwindah |
| author_facet | Syazili Mustofa Ilma Puteri Hutami Dwi Sarwindah |
| author_sort | Syazili Mustofa |
| collection | DOAJ |
| description |
Background: The therapeutic properties of the ethanol extract derived from the stem bark of Rhizophora apiculata have been investigated. Nevertheless, there is a lack of studies regarding its acute toxicity.
Objective: This research aims to evaluate the toxicity of ethanolic extract of R. apiculata bark by examining histological changes in the liver and kidney of rats.
Method: The ethanol extract of R. apiculata bark was administered using a fixed-dose approach in preliminary and primary tests, focusing on the kidneys and liver of male Sprague Dawley rats. Four treatment groups received doses of 5, 50, 300, and 2000 mg/kg BW, while one group served as the control. An uninterrupted observation period of 14 days was conducted to determine any indications of acute toxicity in these animals. On the fifteenth day, the rats were terminated.
Results: This study indicated the absence of any toxic manifestations, such as tremors, excessive salivation, convulsions, coma, and mortality. Nevertheless, it caused histological damage to the rats’ liver and kidney when administered at doses 300 and 2000 mg/kg BW.
Conclusion: While the administration of R. apiculata extracts at doses of 300 and 2000 mg/kgBW resulted in histological damage to the kidneys and livers of the rats, it did not induce any immediate symptoms.
|
| format | Article |
| id | doaj-art-b1d7ac1670fc42f2a04cdc814829ba24 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-b1d7ac1670fc42f2a04cdc814829ba242025-02-08T03:04:51ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222024-07-016210.32889/actabioina.144Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose methodSyazili Mustofa0Ilma Puteri Hutami1Dwi Sarwindah2Department of Biochemistry, Biomolecular and Physiology, Faculty of Medicine, Universitas Lampung, Bandar Lampung 35145, IndonesiaFaculty of Medicine, Universitas Lampung, Bandar Lampung 35145, IndonesiaFaculty of Medicine, Universitas Lampung, Bandar Lampung 35145, Indonesia Background: The therapeutic properties of the ethanol extract derived from the stem bark of Rhizophora apiculata have been investigated. Nevertheless, there is a lack of studies regarding its acute toxicity. Objective: This research aims to evaluate the toxicity of ethanolic extract of R. apiculata bark by examining histological changes in the liver and kidney of rats. Method: The ethanol extract of R. apiculata bark was administered using a fixed-dose approach in preliminary and primary tests, focusing on the kidneys and liver of male Sprague Dawley rats. Four treatment groups received doses of 5, 50, 300, and 2000 mg/kg BW, while one group served as the control. An uninterrupted observation period of 14 days was conducted to determine any indications of acute toxicity in these animals. On the fifteenth day, the rats were terminated. Results: This study indicated the absence of any toxic manifestations, such as tremors, excessive salivation, convulsions, coma, and mortality. Nevertheless, it caused histological damage to the rats’ liver and kidney when administered at doses 300 and 2000 mg/kg BW. Conclusion: While the administration of R. apiculata extracts at doses of 300 and 2000 mg/kgBW resulted in histological damage to the kidneys and livers of the rats, it did not induce any immediate symptoms. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/144acute toxicityfixed dose methodkidney histologyliver histologyRhizophora apiculata |
| spellingShingle | Syazili Mustofa Ilma Puteri Hutami Dwi Sarwindah Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method Acta Biochimica Indonesiana acute toxicity fixed dose method kidney histology liver histology Rhizophora apiculata |
| title | Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method |
| title_full | Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method |
| title_fullStr | Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method |
| title_full_unstemmed | Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method |
| title_short | Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method |
| title_sort | acute toxicity test of rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method |
| topic | acute toxicity fixed dose method kidney histology liver histology Rhizophora apiculata |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/144 |
| work_keys_str_mv | AT syazilimustofa acutetoxicitytestofrhizophoraapiculatabarkextractonratliverandkidneyhistologyusingfixeddosemethod AT ilmaputerihutami acutetoxicitytestofrhizophoraapiculatabarkextractonratliverandkidneyhistologyusingfixeddosemethod AT dwisarwindah acutetoxicitytestofrhizophoraapiculatabarkextractonratliverandkidneyhistologyusingfixeddosemethod |